2004
DOI: 10.1016/j.micres.2004.04.002
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RAPD-PCR analysis of Staphylococcus aureus strains isolated from bovine and human hosts

Abstract: Staphylococcus aureus is one of the most important pathogens in humans and animals. In this study eighty strains were analyzed by RAPD-PCR to assess the genetic relationship between S. aureus isolates from bovine and human hosts. Results were compared with those obtained by biotyping. Fifty-two percent of the S. aureus isolates belonged to a host specific biotype (human, bovine and poultry). Bovine and human ecovars were the most prevalent. Dendrogram obtained by RAPD results showed that all the isolates clust… Show more

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Cited by 53 publications
(56 citation statements)
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“…Amplified fragments of gap and coa genes were digested with AluI restriction enzyme (Fermentas) according to the manufacturer's procedure. RAPD was conducted using primers and PCR conditions reported previously by Reinoso et al (2004). Phylogenetic analysis was determined using UPGMA and Jaccard's coefficient (GeneTools software, Syngene).…”
Section: Methodsmentioning
confidence: 99%
“…Amplified fragments of gap and coa genes were digested with AluI restriction enzyme (Fermentas) according to the manufacturer's procedure. RAPD was conducted using primers and PCR conditions reported previously by Reinoso et al (2004). Phylogenetic analysis was determined using UPGMA and Jaccard's coefficient (GeneTools software, Syngene).…”
Section: Methodsmentioning
confidence: 99%
“…Suşlar arasındaki klonal ilişkinin belirlenmesi amacıyla OLP6 (5'-GAGGGAAGAG-3'), OLP11 (5'-ACGATGAGCC-3') ve OLP13 (5'-ACCGCCTGCT-3') primerleri (Invitrogen, ABD) kullanılarak RAPD PCR uygulandı 11 . PCR ürünleri 10x TBE tamponu içinde 2.5 µl etidyum bromür içeren %1'lik agaroz jelde 150 voltta 25 dk yürütüldükten sonra Gel Logic 2200 Imaging System (Kodak, ABD) ile görüntülendi.…”
Section: Rapd (Random Amplification Of Polymorphic Dna) Analiziunclassified
“…The RW3A primer is a repetitive sequence that permits the genetic distinction of phenotypically equivalent strains of S. aureus (35).…”
Section: S Aureus Is Highly Versatile In Acquiring Antimicrobialmentioning
confidence: 99%