RAPD-PCR analysis of Staphylococcus aureus strains isolated from bovine and human hosts

Reinoso, Elina; Bettera, Susana; Frigerio, Cecilia; DiRenzo, Miguel; Calzolari, A.; Bogni, Cristina

doi:10.1016/j.micres.2004.04.002

Cited by 53 publications

(56 citation statements)

References 19 publications

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“…Amplified fragments of gap and coa genes were digested with AluI restriction enzyme (Fermentas) according to the manufacturer's procedure. RAPD was conducted using primers and PCR conditions reported previously by Reinoso et al (2004). Phylogenetic analysis was determined using UPGMA and Jaccard's coefficient (GeneTools software, Syngene).…”

Section: Methodsmentioning

confidence: 99%

Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis

Karakulska¹,

Pobucewicz²,

Nawrotek³

et al. 2011

Polish Journal of Veterinary Sciences

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The aim of this study was molecular identification of S. aureus strains isolated from mastitic milk samples and establishing the genetic relationship between strains isolated from cows belonging to the same herd. In all 43 isolated strains the gap gene (930 bp) was amplified, which enabled their affiliation to the Staphylococcus genus to be established. PCR-RFLP with AluI endonuclease of the gap gene as well as nuc (450 bp) and coa (1130 bp) gene amplification allowed precise S. aureus species identification. One hundred percent of the genetic relationship between strains was established via RAPD-PCR and coa-typing.

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Section: Methodsmentioning

confidence: 99%

Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis

Karakulska¹,

Pobucewicz²,

Nawrotek³

et al. 2011

Polish Journal of Veterinary Sciences

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“…Suşlar arasındaki klonal ilişkinin belirlenmesi amacıyla OLP6 (5'-GAGGGAAGAG-3'), OLP11 (5'-ACGATGAGCC-3') ve OLP13 (5'-ACCGCCTGCT-3') primerleri (Invitrogen, ABD) kullanılarak RAPD PCR uygulandı 11 . PCR ürünleri 10x TBE tamponu içinde 2.5 µl etidyum bromür içeren %1'lik agaroz jelde 150 voltta 25 dk yürütüldükten sonra Gel Logic 2200 Imaging System (Kodak, ABD) ile görüntülendi.…”

Section: Rapd (Random Amplification Of Polymorphic Dna) Analiziunclassified

Investigation of Macrolide, Lincosamide and Streptogramin B Resistance in Staphylococcus aureus Strains Isolated from Clinical Samples by Phenotypical and Genotypical Methods

Ozansoy¹,

Cevahir²,

Kaleli³

2015

Mikrobiyol Bul

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ÖZStaphylococcus aureus, toplum ve hastane kaynaklı enfeksiyon etkeni olarak en sık izole edilen mikroorganizmalardandır. Stafilokoklar geçmişten günümüze dek gelen süreçte önce penisilinlere ardından metisilin ve glikopeptidler olmak üzere çok sayıda antibiyotiğe direnç geliştirmelerinden ve epidemilere yol açabilmelerinden dolayı önemli bir sorun haline gelmiştir. Metisilin direnci, makrolidler gibi alternatif antibiyotiklerin kullanımına neden olmuş; ancak tüm dünyada gelişen makrolid direnci bu antibiyotiklerin kullanımını sınırlamıştır. Makrolid direnci, hedef bölge değişikliği (MLS B fenotipi, erm genleri tarafından kodlanır), aktif atım pompası (MS fenotipi, msrA/B genleri tarafından kodlanır) veya hücre duvar geçirgenliğinin azalması şeklinde oluşabilir. Bu çalışmada, klinik S.aureus suşlarında MLS B direncinin fenotipik ve genotipik yöntemlerle araştırılması amaçlanmıştır. Çalışmaya, ayaktan (%6.7) ve yatan (%93.3) hastaların yara (%50), trakeal aspirat (%15) ve diğer (%35) örneklerinden izole edilen toplam 404 S.aureus suşu alınmıştır. Suşlarda MLS B direncinin fenotipik olarak araştırılmasında çift disk sinerji testi (D-test), genotipik olarak araştırılmasında ise PCR yöntemi kullanılmıştır. S.aureus suşlarının 158 (%39.1)'i metisiline dirençli (MRSA), 246 (%60.9)'sı metisiline duyarlı (MSSA) olarak bulunmuştur.

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“…The RW3A primer is a repetitive sequence that permits the genetic distinction of phenotypically equivalent strains of S. aureus (35).…”

Section: S Aureus Is Highly Versatile In Acquiring Antimicrobialmentioning

confidence: 99%

Carriage frequency, phenotypic and genotypic characteristics of Staphylococcus aureus isolated from dialysis and kidney tranplant patients at a hosptial in northern Paraná

Giarola¹,

Santos²,

Tognim³

et al. 2012

Braz. J. Microbiol.

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The objective of the present study was to determine the frequency of Staphylococcus aureus nasal carriage among dialysis and kidney transplant patients, to identify the antimicrobial resistance profile of these strains and to verify their genetic profiles with the RW3A primer. The study included 159 individuals, comprising 111 dialysis and 48 kidney transplant patients. Of the 48 transplant patients, 75% were positive for S.aureus, whereas 49% of the 111 dialysis patients were carriers. Two samples yielded conflicting results for oxacillin sensitivity between the disk diffusion and minimum inhibitory concentration (MIC) assays: both were sensitive by the disk diffusion assay and resistant by MIC (4 μg/ml). In the antibiogram by disk diffusion, ten samples were resistant to cefoxitin, among which eight were also resistant to oxacillin. The resistance of the ten samples to cefoxitin by the disk diffusion assay was confirmed by MIC. Of the ten oxacillin-resistant samples, eight harbored the mecA gene. All samples were sensitive to vancomycin, and most were resistant to penicillin and demonstrated high rates of resistance to the other antimicrobials tested.The samples from dialysis patients exhibited a more homogenous genetic profile. Among the samples with a high percent similarity, no correlation with sensitivity or resistance to oxacillin was observed. According to the results of this study, the implementation of prevention and control measures, such as increased restrictions on prescriptions for antimicrobial drugs and nasal decontamination prior to high-risk procedures, is recommended.

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RAPD-PCR analysis of Staphylococcus aureus strains isolated from bovine and human hosts

Cited by 53 publications

References 19 publications

Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis

Molecular typing of Staphylococcus aureus based on PCR-RFLP of coa gene and RAPD analysis

Investigation of Macrolide, Lincosamide and Streptogramin B Resistance in Staphylococcus aureus Strains Isolated from Clinical Samples by Phenotypical and Genotypical Methods

Carriage frequency, phenotypic and genotypic characteristics of Staphylococcus aureus isolated from dialysis and kidney tranplant patients at a hosptial in northern Paraná

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