RAPD markers assisted varietal identification and genetic purity test in pepper, Capsicum annuum

İlbi, Hülya

doi:10.1016/s0304-4238(02)00158-9

Cited by 49 publications

(28 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, the identification of interspecific hybrids relies on both morphological traits and molecular markers. If the hybrid is morphologically similar to one of its parents, molecular markers such as inter-simple sequence repeats (ISSR) and random amplified polymorphic DNA (RAPD) are necessary to help in progeny identification (Ilbi, 2003;Yonemoto et al, 2006;Isshiki et al, 2008;Biswas et al, 2009;Demir et al, 2010). RAPD and ISSR markers have revealed polymorphic DNA bands that differ between genotypes of strawberry.…”

Section: Introductionmentioning

confidence: 99%

Production of interspecific hybrids between commercial cultivars of the eggplant (Solanum melongena L.) and its wild relative S. torvum

Jutamas

Wei²,

Lee³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT.Interspecific hybrids between cultivars of eggplant (Solanum melongena L.) and its wild relative S. torvum, which has disease resistance and desirable traits for crop improvement, were obtained by cross-hybridization and embryo rescue. Twenty-one hybrid progenies were obtained and examined based on morphological traits, RAPD and ISSR markers. Five of them were confirmed to be true interspecific hybrids. Eighteen and 14 bands from 7 RAPD and 14 ISSR primers, respectively, were polymorphic and present in all five hybrid seedlings and their parents. The morphological characteristics of leaf margin, inflorescence type and spine positions of the five seedlings were intermediate to the parents. These interspecific hybrids had low pollen viability, probably due to abnormal meiosis.

show abstract

Section: Introductionmentioning

confidence: 99%

Production of interspecific hybrids between commercial cultivars of the eggplant (Solanum melongena L.) and its wild relative S. torvum

Jutamas

Wei²,

Lee³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…Esta técnica tem auxiliado a identificação de variabilidade genética em diferentes populações de plantas e coleções de germoplasma. Diversos trabalhos em Capsicum que utilizam RAPD têm demonstrado sua utilidade como fonte de informações filogenéticas na identificação de cultivares, em estudos de genealogia, mapeamento genético, distinção entre acessos, identificação de duplicatas, estimativa de taxa de intercruzabilidade, entre outros (INAI et al, 1993;PRINCE et al, 1995;VAZQUEZ et al, 1996;LIVINGSTONE et al, 1999;RODRIGUEZ et al, 1999;LIN et al, 2000;BAOXI et al, 2000;ENGLE et al, 2001;KALOO et al, 2002;ILBI, 2003;PARAN et al, 2004;SUGITA et al, 2005;RUANET et al, 2005;COSTA et al, 2006). Embora seja um marcador de baixa repetibilidade de resultados, ele tem como principais vantagens a simplicidade de utilização, o baixo custo e a rapidez na geração de resultados, o que o torna eficiente e adequado para ser utilizado em estudos de diversidade genética, em coleções de germoplasma, na distinção de acessos e no reconhecimento de materiais duplicados.…”

unclassified

Marcadores RAPD e caracteres morfoagronômicos na determinação da diversidade genética entre acessos de pimentas e pimentões

et al. 2008

View full text Add to dashboard Cite

INTRODUÇÃONo intuito de se estimar a diversidade genética presente em uma coleção de germoplasma, os acessos devem ser caracterizados e avaliados. Essa caracterização pode ser morfoagronômica, citológica, bioquímica, fisiológica ou molecular. Independente do método utilizado, o importante é que os resultados possibilitem uma boa distinção dos acessos e permitam identificar duplicatas e também acessos com características relevantes que possam ser de interesse aos diversos programas de melhoramento. A escolha

show abstract

“…Some workers reported similar results in cultivated tomato [10,14]. The lack of polymorphism has also been reported in other self-pollinated crops such as soybean, potato, and ground nut [7,11,20]. Such insufficient variability in DNA polymorphism makes it difficult to construct a genetic map, if using populations derived from across between cultivars [2].…”

Section: Resultsmentioning

confidence: 75%

“…The progression has greatly increased the responsibility and visibility of those charged with long-term conservation and the use of plant genetic resources [2]. It is very important to define identity, purity, and stability of varieties for breeder's rights protection as well as for an effective seed quality control program [11,13]. Part of the solution to this challenge may lie in the use of powerful, yet relatively simple and inexpensive, molecular techniques to generate information to better organize the useful genetic variation present within a collection or cultivars [5].…”

Section: Introductionmentioning

confidence: 99%

Identification and Genetic Diversity of Korean Tomato Cultivars by RAPD Markers

Huh¹,

Youn²,

Kang³

2011

Journal of Life Science

View full text Add to dashboard Cite

Cultivated tomato, Lycopersicum esculentum, is a very important crop. We selected 36 cultivars and studied them for identification and polymorphism by employing random amplified DNA (RAPD) analysis with 80 oligonucleotide primers. Of the 80 primers, 36 primers (45.0%) were polymorphic. Detection of polymorphism in cultivated tomato opens up the possibility of development of its molecular map by judicious selection of genotypes. Molecular markers can also be used for cultivar identification and protection of the plant breeder's intellectual property rights (plant breeders' rights, PBRs). As an example, DNA polymorphism using OPC-13 primer that did not produce the OPC-13-01 band was only found in Junk Pink and Ailsa Craighp cultivars. OPA-12-03 and OPB-15-07 were fragments specific to the TK-70 cultivar and were absent in other cultivars. DNA polymorphism in cultivated tomato in this study was correlated with a type of inflorescence, although some cultivars had exceptions. These approaches will be useful for developing marker-assisted selection tools for genetic enhancement of the tomato plant for desirable traits.

show abstract

RAPD markers assisted varietal identification and genetic purity test in pepper, Capsicum annuum

Cited by 49 publications

References 15 publications

Production of interspecific hybrids between commercial cultivars of the eggplant (Solanum melongena L.) and its wild relative S. torvum

Production of interspecific hybrids between commercial cultivars of the eggplant (Solanum melongena L.) and its wild relative S. torvum

Marcadores RAPD e caracteres morfoagronômicos na determinação da diversidade genética entre acessos de pimentas e pimentões

Identification and Genetic Diversity of Korean Tomato Cultivars by RAPD Markers

Contact Info

Product

Resources

About