RAPD-based evaluation revealed genetically diverse populations of Pseudomonas aeruginosa and Staphylococcus aureus lytic bacteriophages isolated in urban sewage and Ebrie Lagoon, Cte dIvoire

Addablah, Audrey; Kakou-Ngazoa, Solange; Akpa, Eric Essoh; Adioumani, Emmanuella; Ndombi, Fred M’Bourou; Aoussi, Serge; Dosso, Mireille

doi:10.5897/ajmr2021.9547

Cited by 4 publications

(6 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The broad range of agriphages to other bacteria strains is particular for phages in tropical countries. Previous studies have shown the circulation of polyvalent phages in West Africa (Addablah et al, 2021;Kakou-Ngazoa et al, 2020). Using RADP-PCR methods, the genomic profile of the agriphages allowed the confirmation of 60% of isolated phages such as DNA phages.…”

Section: Discussionmentioning

confidence: 99%

“…Using RADP-PCR methods, the genomic profile of the agriphages allowed the confirmation of 60% of isolated phages such as DNA phages. This is an excellent approach for typing phages infecting bacteria of the same or different species (Gutierrez et al, 2011;Addablah et al, 2021). The genomic profile showed the genetic diversity of the isolated phages.…”

Section: Discussionmentioning

confidence: 99%

“…The RAPD-PCR was performed by using oligonucleotides for random amplification of phages (Gutierrez et al, 2011; Addablah et al, 2021). The PCR was performed with a modification of Kumari's method (Kumari et al, 2009).…”

Section: Random Amplification Of Polymorphic Dnamentioning

confidence: 99%

See 2 more Smart Citations

First Isolation and Identifiaction of Agriphages in Vegetable Crops in West Africa (Cȏte d’Ivorie): Potential Uses of Biocontrol in Plants

Adioumani,

Kakou-Ngazoa,

Trebissou Jonhson

et al. 2023

ESJ

View full text Add to dashboard Cite

Agriphages or bacterial viruses are ubiquitous in the environment. The discovery of virulent phages against phytobacteria improves crop growth and proposes biopesticide uses for plant diseases. In Africa, many phytobacteria such as Ralstonia, Clavibacter, and Xanthomonas were reported in several regions. This paper focuses on evaluating the presence of agriphages for the biocontrol of phytobacteria in Côte d'Ivoire. Leaves and soil samples were collected from healthy and diseased plants in three sites located in Anyama, Abidjan, and Bingerville. The pretreatments occurred with sterile and physiological water for leaves and soil samples, respectively. The isolation of agriphages was done on specific media with Xanthomonas campestris as bacterial host. Lytic activity was tested on agar media for five bacteria strains. After DNA extraction using the Qiagen method kit, molecular confirmation of agriphages was done by Random Amplified Polymorphic DNA-PCR. From this study, five (5) agriphages were isolated in soil and leaves in site 2. These agriphages have all been isolated from Xanthomonas campestris and have a broad spectrum of lytic activity. Molecular characterization by RAPD-PCR showed that three of these agriphages are DNA phages. The dendrogram showed that phages ΦXanS1 and ΦXanS2 have 93% similarities, while ΦXanS1 and ΦXanS2 are 62% similar to ΦXanF1. This study is the first reported agriphages in West Africa, alongside their potential uses against phytobacteria for biocontrol infection in crops.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

First Isolation and Identifiaction of Agriphages in Vegetable Crops in West Africa (Cȏte d’Ivorie): Potential Uses of Biocontrol in Plants

Adioumani,

Kakou-Ngazoa,

Trebissou Jonhson

et al. 2023

ESJ

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Random Amplification Of Polymorphic Dnamentioning

confidence: 99%

Untitled

2023

ESJ

View full text Add to dashboard Cite

show abstract

A New Approach for Controlling Agrobacterium tumefaciens Post Transformation Using Lytic Bacteriophage

Ramadhan

Alfiko²,

Purwantomo³

et al. 2022

Plants

View full text Add to dashboard Cite

Overgrowth of Agrobacterium tumefaciens has frequently been found in Agrobacterium-mediated plant transformation. This overgrowth can reduce transformation efficiency and even lead to explant death. Therefore, this research investigates an alternative way to mitigate or eliminate Agrobacterium after transformation using a bacteriophage. To develop this alternative method, we conducted effectiveness studies of two lytic bacteriophages (ΦK2 and ΦK4) and performed an application test to control Agrobacterium growth after transformation. According to plaque morphological characterization and molecular analysis, the two bacteriophages used in this experiment were distinct. Moreover, some stability physicochemical and growth kinetics, such as adsorption time and susceptibility test, also showed that both bacteriophages differed. On the other hand, the optimum temperature and pH of both phages were the same at 28–30 °C and pH 7. Further investigation showed that both ΦK2 and ΦK4 were able to reduce the overgrowth of A. tumefaciens post transformation. Moreover, applying the cocktail (mixture of ΦK2 and ΦK4) with antibiotic application eradicated A. tumefaciens (0% overgrowth percentage). This result indicates that the application of bacteriophage could be used as an alternative way to eradicate the overgrowth of A. tumefaciens subsequent to transformation.

show abstract

RAPD-based evaluation revealed genetically diverse populations of Pseudomonas aeruginosa and Staphylococcus aureus lytic bacteriophages isolated in urban sewage and Ebrie Lagoon, Cte dIvoire

Cited by 4 publications

References 34 publications

First Isolation and Identifiaction of Agriphages in Vegetable Crops in West Africa (Cȏte d’Ivorie): Potential Uses of Biocontrol in Plants

First Isolation and Identifiaction of Agriphages in Vegetable Crops in West Africa (Cȏte d’Ivorie): Potential Uses of Biocontrol in Plants

Untitled

A New Approach for Controlling Agrobacterium tumefaciens Post Transformation Using Lytic Bacteriophage

Contact Info

Product

Resources

About