RAPD analysis of Aeromonas salmonicida and Aeromonas hydrophila

Abstract

The randomly amplified polymorphic DNA (RAPD) technique was used to analyse the genetic differentiation of 13 strains of Aeromonas salmonicida subsp. salmonicida, and seven strains of Aer. hydrophila. Reproducible profiles of genomic DNA fingerprints were generated by polymerase chain reaction (PCR) using a single randomly designed primer. The RAPD profiles of all the non-motile aeromonads, Aer. salmonicida subsp. salmonicida were identical. However, profiles of the motile aeromonads, Aer. hydrophila differed between isolates. These findings reveal genomic homogeneity in Aer. salmonicida subsp. salmonicida and genetic variety in Aer. hydrophila strains.

Aeromonas aquariorum Is Widely Distributed in Clinical and Environmental Specimens and Can Be Misidentified as Aeromonas hydrophila

“…Results obtained in the present study may help to explain the biochemical and genotypic heterogeneity previously observed in A. hydrophila (6,11 a Miscellaneous specimens include A. aquariorum from bone chip (1), urine (2), sputum (1), and unknown (1) specimens, A. caviae from dialysis fluid (1), peritoneal fluid (1), bile (2), T-tube tip (1), and unknown (2) specimens, A. hydrophila from tissue (1), sputum (2), biliary shunt (1), bile (1), pancreatic cyst (1), drain fluid (1), and mortuary (1) specimens, and A. veronii bv. sobria from sputum (2) and appendix (1) specimens.…”

Genotyping Techniques for Determining the Diversity of Microorganisms

“…It has been successfully applied in the genetic differentiation of Salmonella (Tikoo, 2001), E. coli (Renqua-Mangia et al, 2004), A. baumannii (Reboli et al, 1994), S. marcescens (Debast et al, 1995), Proteus mirabilis (Bingen et al, 1993), Enterobacter cloacae (Hou et al, 1997), Haemophilus somnus (Myers et al, 1993), Leptospira species (Ralph et al, 1993), L. pneumophilia (Tram et al, 1990, Van Belkum et al, 1993, B. pseudomallei (Haase et al, 1995, Leelayuwet et al, 2000, Aeromonas salmoniciola (Miyata et al, 1995), Aeromonas hydrophila (Miyata et al, 1995), Vibrio cholerae (Leal et al, 2004), S. aureus (Saulnier et al, 1993), Lactobacillus plantarum (Elegado et al, 2004;Lawrence et al, 1993), Bacillus cereus (Svensson et al, 2004), Listeria monocytogenes (Hiroshi et al, 2007), Candida albicans (Lehmann et al, 1992, Robert et al, 1995, Histoplasma capsulatum (Kersulyte et al, 1992) and Cryptococcus neoformans (Yamamoto et al, 1995). RAPD is highly discriminatory and therefore especially useful in the investigation of short term or local outbreaks of disease.…”

RAPD Analysis of Atypical Aeromonas salmonicida Isolated in Japan.

“…Diagnostic methods need to be highly specific and sensitive. Miyata et al (1995) demonstrated genomic homogeneity in A. salmonicida subsp. salmonicida strains using the randomly amplified polymorphic DNA (RAPD) method (Mafia et al, 1994).…”

“…DNA preparation Genomic DNAs were isolated by the previously described method (Miyata et al, 1995). The concentra tion of the purified DNA was determined with a Gene Quant system (Pharmacia, Sweden).…”