Rapamycin protects dopaminergic neurons against rotenone-induced cell death in primary mesencephalic cell culture

Radad, Khaled; Moldzio, Rudolf; Rausch, Wolf‐Dieter

doi:10.5114/fn.2015.54426

Cited by 19 publications

(14 citation statements)

References 37 publications

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“…These events attributed to increased production of H 2 O 2 , which occurred in the present ROT (47). On the other hand, ROT caused mitochondrial damage, which resulted in apoptotic cell death by disrupting cellular energy metabolism and enhancing ROS production, inhibition of the microtubular network, and condensation/fragmentation of the cell nucleus (48).…”

Section: Discussionmentioning

confidence: 96%

Olive Leaves Extract Improved Sperm Quality and Antioxidant Status in the Testis of Rat Exposed to Rotenone

2017

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Section: Discussionmentioning

confidence: 96%

Olive Leaves Extract Improved Sperm Quality and Antioxidant Status in the Testis of Rat Exposed to Rotenone

2017

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“…In our experiments, primary mesencephalic cell cultures were prepared from the OF1/SPF embryos (250 embryos) at gestation day 14. After collecting the embryos under aseptic condition in Dulbecco's phosphate buffered saline (DPBS, Invitrogen, Germany), brains were released, mesencephala were excised and cultures were prepared according to Radad et al [17]. Briefly, mesencephala were carefully cleaned from meninges, and enzymatically and mechanically dissociated using 0.2% trypsin solution (Invitrogen, Germany) and fire-polished Pasteur pipettes, respectively.…”

Section: Materials and Methods Preparation Of Primary Mesencephalic Cementioning

confidence: 99%

Neurotoxic effects of domoic acid on dopaminergic neurons in primary mesencephalic cell culture

Radad¹,

Al‐Shraim²,

Al-Emam³

et al. 2018

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Introduction: Domoic acid is a potent marine neurotoxin produced by certain species of the diatom genus Pseudonitzschia. To our knowledge, there are no studies that have investigated neurotoxic effects of domoic acid on dopaminergic neurons. Accordingly, the present study was carried out to investigate the potential neurotoxic effects of domoic acid on dopaminergic neurons in primary mesencephalic cell culture. Material and methods: Cultures prepared from embryonic mouse mesencephala (total of 250 embryos) were treated with different concentrations of domoic acid (0.1, 1, 10, 100 µM) on the 10 th DIV for 48 h. On the 12 th DIV, culture media were used for measurement of lactate dehydrogenase and cultured cells were subjected to immunostaining for tyrosine hydroxylase, neuronal nuclear antigen and glial fibrillary acidic protein, and fluorescence staining using H 2 DCFDA, JC-1 and DAPI stains. Moreover, roles of AMPA/KA and NMDA receptors in domoic acid neurotoxicity were also investigated. Results: Domoic acid significantly decreased the number of dopaminergic neurons, decreased the expression of neuronal nuclear antigen and slightly affected astrocyte populations, and increased the release of lactate dehydrogenase into the culture media. AMPA/KA receptor antagonist NBQX but not NMDA receptor antagonist MK-801 significantly inhibited the neurotoxic effect of domoic acid on dopaminergic neurons. H 2 DCFDA, JC-1 and DAPI fluorescence staining, respectively, revealed that DomA slightly raised ROS production, and significantly decreased mitochondrial membrane potential and increased apoptotic cell death of cultured cells. Conclusion: The current study presents for the first time the neurotoxic effects of domoic acid on dopaminergic neurons and this effect appears to be attributed to activation of AMPA/KA receptors on dopaminergic neurons.

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“…Apart of motor clinical signs, these rotenone in vitro models of PD presented most of the cellular and molecular pathology that occurs in PD patients, most notably dopaminergic cell loss (Fig. 1) [55] and formation of protein aggregates containing α-synuclein [80]. Unlike MPP + which specifically damages dopaminergic neurons, rotenone was shown to injure other neuronal populations in primary mesencephalic cell culture.…”

Section: Rotenone Model Of Parkinson's Diseasementioning

confidence: 98%

Rotenone: from modelling to implication in Parkinson’s disease

Radad

Al‐Shraim

Al-Emam

et al. 2019

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Rotenone ([2R-(2α,6aα,12aα)]-1,2,12,12a-tetrahydro-8,9-dimethoxy-2-(1-methylethenyl)-[1]benzopyran[3,4-b]furo [2,3-h][1]benzopyran-6(6aH)-one) is a naturally occurring compound derived from the roots and stems of Derris, Tephrosia, Lonchocarpus and Mundulea plant species. Since its discovery at the end of the 19 th century, rotenone has been widely used as a pesticide for controlling insects, ticks and lice, and as a piscicide for management of nuisance fish in lakes and reservoirs. In 2000, Betarbet et al. reproduced most of the behavioural, biochemical and pathological features of Parkinson's disease (PD) in rotenone-treated rats. Since that time, rotenone has received much attention as it would be one of the environmental neurotoxins implicated in etiopathogenesis of PD. Moreover, it represents a common experimental model to investigate the underlying mechanisms leading to PD and evaluate the new potential therapies for the disease. In the current general review, we aimed to address recent advances in the hazards of the environmental applications of rotenone and discuss the updates on the rotenone model of PD and whether it is implicated in the etiopathogenesis of the disease.

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Rapamycin protects dopaminergic neurons against rotenone-induced cell death in primary mesencephalic cell culture

Abstract: A b s t r a c t

Cited by 19 publications

References 37 publications

Olive Leaves Extract Improved Sperm Quality and Antioxidant Status in the Testis of Rat Exposed to Rotenone

Olive Leaves Extract Improved Sperm Quality and Antioxidant Status in the Testis of Rat Exposed to Rotenone

Neurotoxic effects of domoic acid on dopaminergic neurons in primary mesencephalic cell culture

Rotenone: from modelling to implication in Parkinson’s disease

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