Neurotoxic effects of domoic acid on dopaminergic neurons in primary mesencephalic cell culture

Radad, Khaled; Al‐Shraim, Mubarak; Al-Emam, Ahmed; Moldzio, Rudolf; Rausch, Wolf‐Dieter

doi:10.5114/fn.2018.74658

Cited by 3 publications

(3 citation statements)

References 22 publications

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“…Unlike MPP + which specifically damages dopaminergic neurons, rotenone was shown to injure other neuronal populations in primary mesencephalic cell culture. Similar results were obtained by our laboratory for some other neurotoxins like domoic acid [56] and acrylamide [57].…”

Section: Rotenone Model Of Parkinson's Diseasesupporting

confidence: 89%

Rotenone: from modelling to implication in Parkinson’s disease

Radad

Al‐Shraim

Al-Emam

et al. 2019

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Rotenone ([2R-(2α,6aα,12aα)]-1,2,12,12a-tetrahydro-8,9-dimethoxy-2-(1-methylethenyl)-[1]benzopyran[3,4-b]furo [2,3-h][1]benzopyran-6(6aH)-one) is a naturally occurring compound derived from the roots and stems of Derris, Tephrosia, Lonchocarpus and Mundulea plant species. Since its discovery at the end of the 19 th century, rotenone has been widely used as a pesticide for controlling insects, ticks and lice, and as a piscicide for management of nuisance fish in lakes and reservoirs. In 2000, Betarbet et al. reproduced most of the behavioural, biochemical and pathological features of Parkinson's disease (PD) in rotenone-treated rats. Since that time, rotenone has received much attention as it would be one of the environmental neurotoxins implicated in etiopathogenesis of PD. Moreover, it represents a common experimental model to investigate the underlying mechanisms leading to PD and evaluate the new potential therapies for the disease. In the current general review, we aimed to address recent advances in the hazards of the environmental applications of rotenone and discuss the updates on the rotenone model of PD and whether it is implicated in the etiopathogenesis of the disease.

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Section: Rotenone Model Of Parkinson's Diseasesupporting

confidence: 89%

Rotenone: from modelling to implication in Parkinson’s disease

Radad

Al‐Shraim

Al-Emam

et al. 2019

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“…On gestation day 14, pregnant mice were killed, the uteri were dissected and the embryos were carefully removed to a Petri dish containing Dulbecco's phosphate buffered saline (DPBS, Invitrogen, Germany, pH 7.2). Brains were then obtained, mesencephala were excised and cultures were prepared according to Radad et al [20]. In brief, mesencephala were cleaned from meninges and cut into small pieces in a drop of DPBS.…”

Section: Materials and Methods Preparation Of Primary Mesencephalic Cementioning

confidence: 99%

Neurotoxic effects of acrylamide on dopaminergic neurons in primary mesencephalic cell culture

Radad

Al‐Shraim

Al-Emam

et al. 2019

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Introduction: Exposure to acrylamide is increasing worldwide as a result of its heavy use in industry and formation in carbohydrate-rich food cooked at high temperature. Despite its neurotoxicity, no studies have shown its toxic effects on dopaminergic neurons yet. Therefore, the current study was carried out to show whether acrylamide adversely affects primary cultured dopaminergic neurons. Material and methods: Acrylamide (0.001, 0.01, 0.1, 1, 2 mM) was added to two different groups of primary mesencephalic cell cultures on the 9 th day in vitro for 24 and 48 h, respectively. Moreover, a group of cultures was treated with lower concentrations of acrylamide (0.01, 0.05, 0.1, 0.5 mM) on the 6 th day in vitro for 5 consecutive days to investigate its long-term effects on dopaminergic neurons. Following each treatment, culture media were obtained for measuring lactate dehydrogenase, and cultured cells were stained immunocytochemically against tyrosine hydroxylase and neuronal nuclear antigens. Results: Treatment of cultures with acrylamide for 48 h significantly reduced the number of dopaminergic neurons, adversely altered the morphology of the surviving neurons and increased levels of lactate dehydrogenase in the culture media. Similar treatment of cultures with acrylamide also resulted in lower numbers of total neuronal cells as shown by a reduced expression of the neuronal nuclear antigen. Prolonged treatment of cultures with lower concentrations of acrylamide slightly reduced the survival of dopaminergic neurons but increased the release of lactate dehydrogenase into the culture media as well. Conclusions: The current study shows, for the first time, neurotoxicity of acrylamide on dopaminergic neurons in the primary mesencephalic cell culture.

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“…DA can not only induce persistent epilepsy in rats but also promote aggressive behavior in rats [54]. Significant destruction of dopaminergic neurons is shown after long-term treatment of primary mesencephalic cell culture with DA [55]. Besides its toxicity on the nervous system, DA may also exert toxicological effects on other organs since most of the human organs have some level of glutamate receptor expression [56].…”

Section: Toxicological Impacts Of Damentioning

confidence: 99%

The Impact of Climate Change on The Bioavailability of Environmental Toxins and Their Toxicological Effects

Geng¹,

Li²

2022

HSET

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Climate change has become one of the biggest challenges for the 21st century. Global warming, sea level rise, changes in weather conditions, and atmospheric contamination caused by climate change not only have impacts on nature but also present threats to human health. Climate change can influence the generations and distribution of some representative toxic compounds with specific examples, including heavy metals (arsenic and manganese), persistent organic pollutants (POPs), air pollutants, and biotoxins (domoic acid and β-N-methylamino-L-alanine). This review discusses how the effects of climate change drive the production and spread of toxic substances that enter the human body through different means as well as presents the biochemical mechanisms and experimental evidence of their toxicological effect on human health, which provides an insight on climate change allowing toxic substances to enter human body and calls on readers to understand and pay attention to climate change from a more comprehensive aspect

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Neurotoxic effects of domoic acid on dopaminergic neurons in primary mesencephalic cell culture

Cited by 3 publications

References 22 publications

Rotenone: from modelling to implication in Parkinson’s disease

Rotenone: from modelling to implication in Parkinson’s disease

Neurotoxic effects of acrylamide on dopaminergic neurons in primary mesencephalic cell culture

The Impact of Climate Change on The Bioavailability of Environmental Toxins and Their Toxicological Effects

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