Random/aligned electrospun PCL/PCL-collagen nanofibrous membranes: comparison of neural differentiation of rat AdMSCs and BMSCs

Abstract

In this study, the aligned (A) and randomly oriented (R) polycaprolactone (PCL-A and PCL-R) and PCL/collagen (PCL/Col-A and PCL/Col-R) nanofibers were electrospun onto smooth PCL membranes (PCLMs) prepared by solvent casting. In order to investigate the effects of chemical composition and nanotopography of fibrous surfaces on proliferation and on neural differentiation of mesenchymal stem cells (MSCs), adipose and bone marrow-derived rat MSCs (AdMSCs and BMSCs) were cultivated in suitable media i.e. inducing medium containing basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF), and cell maintenance medium (CMM). BMSCs adhered and proliferated on all nanofibrous membranes more efficiently than AdMSCs. PCL/Col-A was found as the most convenient surface supporting proliferation in both cell types. Immunofluorescence staining indicated that BMSCs and AdMSCs are prone for differentiation to oligodendrocytes more than they differentiate to other neuronal cell types. PCL-A nanofibrous membranes supported differentiation of MSCs to O4(+) (an oligodendrocytes surface antigen) cells in both culture media. The intensity of immunoreactivity of O4(+) cells differentiated from BMSCs on PCL-A was highest when compared with the other groups (p < 0.001). Some BIII-T signed neural cells were investigated on PCL-A nanofibrous membranes, but the intensity of immunoreactivity was lower than that of O4(+) cells. In conclusion, this study can be evaluated to establish the cell therapy strategies in neurodegenerative disorders, which are relevant to oligodendrocyte abstinence using BMSCs or AdMSCs on aligned nanofibrous membranes.

Comparison of the osteogenic differentiation capacity of adipose tissuederived mesenchymal stem cells from humans and rats

“…Recently, several experiments have been performed to demonstrate the existence of adipose tissue-derived progenitors that give rise to bone (Liu et al, 2007;Arrigoni et al, 2009;Song et al, 2011;Çapkın, 2012). It was reported in these studies that various bioactive factors, including dexamethasone, ascorbic acid, and β-glycerophosphate, induced osteogenic differentiation in both human and rat ADMSCs.…”

Neuroprotective Effect of Transplanted Neural Precursors Embedded on PLA/CS Scaffold in an Animal Model of Multiple Sclerosis

“…Based on previous reports using adherent cells on nanofibrous scaffolds [13,14], we hypothesize that the PC12 cells will attach to the polylactic acid/chitosan (PLA/ CS) scaffold which may then be transplanted into areas requiring tissue regeneration. In living systems, extracellular matrix (ECM) plays a pivotal role in controlling cell behavior, while scaffold design plays a pivotal role in tissue engineering [15,16].…”

Thymosin β4 coated nanofiber scaffolds for the repair of damaged cardiac tissue

“…After fabrication any nanoscaffold construct can be subjected to coating, allowing the fiber to display novel researcher-selected properties such as hydrophobicity, bioactivity, or fluorescence [18]. The bioactive coatings can significantly enhance cell growth, cell signaling, and in vivo biocompatibility [19].…”

“…Cells were extracted as described in [18], in which collagenase digestion of murine left ventricles was followed by centrifugal separation. Cultures were prepared in Cardiomyocyte Differentiation Medium (StemCell Technologies) and seeded on two scaffolds: One on a nanofiber scaffold treated with a thymosin β4 coating; the other was prepared on an uncoated scaffold for comparison.…”