ramR mutations affecting fluoroquinolone susceptibility in epidemic multidrug-resistant Salmonella enterica serovar Kentucky ST198

Baucheron, Sylvie; Hello, Simon Le; Doublet, Benoît; Giraud, Étienne; Weill, François‐Xavier; Cloeckaert, Axel

doi:10.3389/fmicb.2013.00213

Cited by 27 publications

(26 citation statements)

References 21 publications

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“…Statistical analysis revealed that the mutations of gyrA (Ser83Phe, Asp87Gly) and parC (Ser80Ile) have a major influence on the high level of drug resistance of quinolones (ciprofloxacin, nalidixic acid, and ofloxacin). As can be seen in this study, quinolone resistance caused by the quinolone resistance-determination region mutation was the same as the internationally common ST198 resistance mechanism [26,27]. It is well known that quinolone-resistant ST198 is widespread in China, both in human and livestock sources.…”

Section: Discussionsupporting

confidence: 53%

“…It has been shown that S. Kentucky has been increasing its multidrug resistance rate since 2014. Fluoroquinolones are the preferred method of clinical treatment for nontyphoid salmonellosis [26]; at the same time, they are the main reasons for the spread of ST198 in the world [27]. The most important targets of fluoroquinolones for microorganisms are DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC and parE); the mutations in the gyrA and parC genes are the most common mechanisms of bacterial resistance to quinolone antibiotics generally [28].…”

Section: Discussionmentioning

confidence: 99%

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Ciprofloxacin-Resistant Salmonella enterica Serovar Kentucky ST198 in Broiler Chicken Supply Chain and Patients, China, 2010–2016

et al. 2020

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Salmonella enterica serovar Kentucky (S. Kentucky) sequence type 198 has emerged as a global zoonotic pathogen. We explored Salmonella enterica serovar Kentucky ST198 samples from the broiler chicken supply chain and patients between 2010 and 2016. Here, we collected 180 S. Kentucky isolates from clinical cases and the poultry supply chain. We performed XbaI pulsed-field gel electrophoresis and multilocus sequence typing. We assessed mutations in the quinolone resistance-determining regions and screened for the presence of the Salmonella genomic island 1 (SGI1). We determined that 63 (35.0%) of the 180 isolates were S. Kentucky ST198. Chinese strains of S. Kentucky ST198 have a high transmission of ciprofloxacin resistance (38/63, 60.3%) and a high risk of multidrug resistance. The quinolone resistance of the S. Kentucky ST198 strain found in China may be due to mutations in its quinolone resistance-determining region. Our study firstly revealed that ciprofloxacin-resistant S. Kentucky ST198 strains can undergo cross-host transmission, thereby causing a serious foodborne public health problem in China.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 99%

Ciprofloxacin-Resistant Salmonella enterica Serovar Kentucky ST198 in Broiler Chicken Supply Chain and Patients, China, 2010–2016

et al. 2020

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“…Antibiotic resistance conferring plasmids are frequently identified in poultry-associated S . Kentucky ST152 isolates [ 12 ] while antibiotic resistance in human clinical S. Kentucky ST198 is associated with acquisition of the Salmonella Genomic Island 1 (SGI1), plasmids, and core genome polymorphisms [ 7 ][ 9 ][ 44 ][ 45 ].…”

Section: Resultsmentioning

confidence: 99%

Genomic and Evolutionary Analysis of Two Salmonella enterica Serovar Kentucky Sequence Types Isolated from Bovine and Poultry Sources in North America

et al. 2016

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Salmonella enterica subsp. enterica serovar Kentucky is frequently isolated from healthy poultry and dairy cows and is occasionally isolated from people with clinical disease. A genomic analysis of 119 isolates collected in the United States from dairy cows, ground beef, poultry and poultry products, and human clinical cases was conducted. Results of the analysis demonstrated that the majority of poultry and bovine-associated S. Kentucky were sequence type (ST) 152. Several bovine-associated (n = 3) and food product isolates (n = 3) collected from the United States and the majority of human clinical isolates were ST198, a sequence type that is frequently isolated from poultry and occasionally from human clinical cases in Northern Africa, Europe and Southeast Asia. A phylogenetic analysis indicated that both STs are more closely related to other Salmonella serovars than they are to each other. Additionally, there was strong evidence of an evolutionary divergence between the poultry-associated and bovine-associated ST152 isolates that was due to polymorphisms in four core genome genes. The ST198 isolates recovered from dairy farms in the United States were phylogenetically distinct from those collected from human clinical cases with 66 core genome SNPs differentiating the two groups, but more isolates are needed to determine the significance of this distinction. Identification of S. Kentucky ST198 from dairy animals in the United States suggests that the presence of this pathogen should be monitored in food-producing animals.

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“…A total of 97 S. Kentucky ST198 isolates were directly analysed in this study (Table S1), including 68 isolates collected between 1937 and 2013 that were previously studied by conventional molecular methods (3)(4)(5)23), and 29 new isolates collected between 2008 and 2016. These isolates originated from the French National Reference Center for E. coli, Shigella, Salmonella (Institut Pasteur) and several other international laboratories and were selected on the basis of their diversity (human or non-human source, geographic area and year of isolation, PFGE types, and antimicrobial resistance phenotypes and genotypes).…”

Section: Bacterial Isolates Used In This Studymentioning

confidence: 99%

Global phylogenomics of multidrug resistant Salmonella enterica serotype Kentucky ST198

Hawkey

Hello

Doublet

et al. 2019

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Salmonella enterica serotype Kentucky (S. Kentucky) can be a common causative agent of salmonellosis, usually associated with consumption of contaminated poultry. Antimicrobial resistance (AMR) to multiple drugs, including ciprofloxacin, is an emerging problem within this serotype. We used whole-genome sequencing (WGS) to investigate the phylogenetic structure and AMR content of 121 S. Kentucky ST198 isolates from five continents. Population structure was inferred using phylogenomic analysis and whole genomes were compared to investigate changes in gene content, with a focus on acquired AMR genes. Our analysis showed that multidrug resistant (MDR) S. Kentucky isolates belonged to a single lineage, which we estimate emerged circa 1989 following the acquisition of the AMR-associated Salmonella genomic island 1 (variant SGI1-K) conferring resistance ampicillin, streptomycin, gentamicin, sulfamethoxazole, and tetracycline. Phylogeographic analysis indicates this clone emerged in Egypt before disseminating into Northern, Southern and Western Africa, then to the Middle East, Asia and the European Union. The MDR clone has since accumulated various substitution mutations in the quinolone resistance determining regions (QRDR) of DNA gyrase (gyrA) and DNA topoisomerase (parC), such that most strains carry three QRDR mutations which together confer resistance to ciprofloxacin. The majority of AMR genes in the S. Kentucky genomes were carried either on plasmids or SGI structures. Remarkably, each genome carried a different SGI1-K derivative structure; this variation could be attributed to IS26-mediated insertions and deletions, which appear to have hampered previous attempts to trace the clone's evolution using sub-WGS resolution approaches. Several different AMR plasmids were also identified, encoding resistance to chloramphenicol, third-generation cephalosporins, carbapenems, and/or azithromycin. These results indicate that most MDR S. Kentucky circulating globally result from the clonal expansion of a single lineage that acquired chromosomal AMR genes 30 years ago, and has continued to diversify and accumulate additional resistances to last-line oral antimicrobials.

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ramR mutations affecting fluoroquinolone susceptibility in epidemic multidrug-resistant Salmonella enterica serovar Kentucky ST198

Cited by 27 publications

References 21 publications

Ciprofloxacin-Resistant Salmonella enterica Serovar Kentucky ST198 in Broiler Chicken Supply Chain and Patients, China, 2010–2016

Ciprofloxacin-Resistant Salmonella enterica Serovar Kentucky ST198 in Broiler Chicken Supply Chain and Patients, China, 2010–2016

Genomic and Evolutionary Analysis of Two Salmonella enterica Serovar Kentucky Sequence Types Isolated from Bovine and Poultry Sources in North America

Global phylogenomics of multidrug resistant Salmonella enterica serotype Kentucky ST198

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