Raman tracking the activity of urease in saliva for healthcare

Hu, Sen; Gao, Yun; Wu, Yiping; Guo, Xiaoyu; Ye, Ying; Wen, Ying; Yang, Haifeng

doi:10.1016/j.bios.2018.12.059

Cited by 26 publications

(13 citation statements)

References 31 publications

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“…SERS peak due to urea present at 1003 cm −1 can be used to detect the presence of urease in oral cavity fluid. An intensity decline in this band was observed in saliva spiked with urease (Hu et al 2019 ). Zamora-Mendoza et al have carried out SERS-based non-invasive diagnosis of asthma by identifying biomarkers in saliva.…”

Section: Optical Spectroscopy Techniques For Salivamentioning

confidence: 90%

“…Sjögren's syndrome (SjS) 2020 [Moisoiu 2020] 96.5% 2 ATR-IR Periodontal Diseases 2020 [Beyer-Hans et al 2020 ] _ 3 ATR-FTIR Bio fluids including saliva; Review, clinical applications 2020 [Naseer et al 2020 ] _ 4 Raman Amyotrophic Lateral Sclerosis (ALS) diagnosis 2020 [Carlomagno et al 2020 ] _ 5 SERS Periodontitis, Sialic Acid (SA) 2019 [Hernández-Cedillo et al 2019 ] 69.6% 6 SERS To evaluate the activity of urease in saliva. Dental caries; helicobacter pylori infection 2019 [Hu et al 2019 ] L.O.D: 2.35 μg/ml 7 SERS biomarkers of bronchial inflammation in the saliva of children with asthma 2019 [Zamora-Mendoza et al 2019 ] 85% 8 ATR-FTIR Chronic kidney disease 2019 [Rodrigues et al 2019a , b ] 92.8% 9 FTIR Burning Mouth Syndrome 2019 [Rodrigues et al 2019a , b ] _ 10 FTIR Neonatal sepsis using saliva 2019 [Yunanto et al 2019 ] _ 11 ATR-FTIR Multiple dental caries 2018 [Seredin et al 2018 ] _ 12 Plasmonics Hepatitis B 2017 [Riedel et al 2017 ] _ 13 IR Saliva in healthy, chronic, and aggressive periodontitis 2017 [Saranya …”

Section: Summary and Future Prospectsmentioning

confidence: 99%

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Photonics of human saliva: potential optical methods for the screening of abnormal health conditions and infections

Lukose

Mithun

et al. 2021

Biophys Rev

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Human saliva can be treated as a pool of biological markers able to reflect on the state of personal health. Recent years have witnessed an increase in the use of optical devices for the analysis of body fluids. Several groups have carried out studies investigating the potential of saliva as a non-invasive and reliable clinical specimen for use in medical diagnostics. This brief review aims to highlight the optical technologies, mainly surface plasmon resonance (SPR), Raman, and Fourier transform infrared (FTIR) spectroscopy, which are being used for the probing of saliva for diverse biomedical applications. Advances in bio photonics offer the promise of unambiguous, objective and fast detection of abnormal health conditions and viral infections (such as COVID-19) from the analysis of saliva.

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Section: Optical Spectroscopy Techniques For Salivamentioning

confidence: 90%

Section: Summary and Future Prospectsmentioning

confidence: 99%

Photonics of human saliva: potential optical methods for the screening of abnormal health conditions and infections

Lukose

Mithun

et al. 2021

Biophys Rev

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“…Many urinary and gastrointestinal diseases including cancer and pyelonephritis are closely related to urease activity, and the sensing of urease activity has been used to diagnose some diseases . Noteworthily, the urease activity in the oral cavity is recognized as an important diagnostic biomarker for early disease diagnosis . Up to date, various methods such as fluorescence, ELISA, electrochemistry, and colorimetric , were applied for the analysis of urease.…”

Section: Introductionmentioning

confidence: 99%

“…5 Noteworthily, the urease activity in the oral cavity is recognized as an important diagnostic biomarker for early disease diagnosis. 6 Up to date, various methods such as fluorescence, 7 ELISA, 8 electrochemistry, 9 and colorimetric 10,11 were applied for the analysis of urease. Nevertheless, the development of an advanced functional dual-modal photoelectrochemical (PEC) and electrochemical (EC) sensing platform remains meaningful for urease detection.…”

Section: ■ Introductionmentioning

confidence: 99%

Proton-Regulated Catalytic Activity of Nanozymes for Dual-Modal Bioassay of Urease Activity

et al. 2021

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Benefiting from the merits of high stability and superior activity, nanozymes are recognized as promising alternatives to natural enzymes. Despite the great leaps in the field of therapy and colorimetric sensing, the development of highly sensitive nanozyme-involved photoelectrochemical (PEC) biosensors is still in its infancy. Specifically, the investigation of multifunctional nanozymes facilitating different catalytic reactions remains largely unexplored due to the difficulty in synergistically amplifying the PEC signals. In this work, mesoporous trimetallic AuPtPd nanospheres were synthesized with both efficient oxidase and peroxidase-like activities, which can synergistically catalyze the oxidation of 4-chloro-1-naphthol to produce benzo-4-chlorohexadienone precipitation on the surface of photoactive materials, and thus lead to the decreased photocurrent as well as increased charge-transfer resistance. Inspired by the proton-dependent catalytic activity of nanozymes, a self-regulated dual-modal PEC and electrochemical bioassay of urease activity was innovatively established by in situ regulating the activity of AuPtPd nanozymes through urease-mediated proton-consuming enzymatic reactions, which can remarkably improve the accuracy of the assay. Meanwhile, the determination of urease activity in spiked human saliva samples was successfully realized, indicating the reliability of the biosensor and its application prospects in clinical diagnosis.

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“…SERS methods have been developed as a promising analytical approach with the advantages of a high sensitivity and rich vibration fingerprint information. − Furthermore, some reaction-based SERS strategies have been developed to sensitively detect the inorganic molecule with a small scattering cross section. For example, 3,4-diaminobenzene-thiol as the SERS biosensor can realize the imaging of NO in living cells, and the SERS nanosensor based on the Au/Ag nanobox was designed for the detection of H 2 S in living cells and zebrafish embryos .…”

mentioning

confidence: 99%

Simultaneous Detection of Intracellular Nitric Oxide and Peroxynitrite by a Surface-Enhanced Raman Scattering Nanosensor with Dual Reactivity

et al. 2019

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A functional surface-enhanced Raman scattering (SERS) nanosensor which can simultaneously detect nitric oxide (NO) and peroxynitrite (ONOO–) in living cells is explored. The SERS nanosensor is fabricated through modifying gold nanoparticles (AuNPs) with newly synthesized 3,4-diaminophenylboronic acid pinacol ester (DAPBAP), which has two reactive groups. The simultaneous detection achieved in this work is not only because of the SERS spectral changes of the nanosensor resulting from the dual reactivity of DAPBAP on AuNPs with NO and ONOO– but also by the narrow SERS bands suitable for multiplex detection. Owing to the combination of SERS fingerprinting information and chemical reaction specificity, the nanosensor has great selectivity for NO and ONOO–, respectively. In addition, the nanosensor has a wide linearity range from 0 to 1.0 × 10–4 M with a submicromolar sensitivity. More importantly, simultaneous monitoring of NO and ONOO– in the Raw264.7 cells has been fulfilled by this functional nanosensor, which shows that the SERS strategy will be promising in comprehension of the physiological issues related with NO and ONOO–.

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Raman tracking the activity of urease in saliva for healthcare

Cited by 26 publications

References 31 publications

Photonics of human saliva: potential optical methods for the screening of abnormal health conditions and infections

Photonics of human saliva: potential optical methods for the screening of abnormal health conditions and infections

Proton-Regulated Catalytic Activity of Nanozymes for Dual-Modal Bioassay of Urease Activity

Simultaneous Detection of Intracellular Nitric Oxide and Peroxynitrite by a Surface-Enhanced Raman Scattering Nanosensor with Dual Reactivity

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