Raman spectrum reveals the cell cycle arrest of Triptolide-induced leukemic T-lymphocytes apoptosis

Zhang, Dao-Sen; Feng, Yanyan; Zhang, Qinnan; Su, Xin; Lu, Xiaoxu; Liu, Shengde; Zhong, Liu

doi:10.1016/j.saa.2015.01.037

Cited by 17 publications

(14 citation statements)

References 34 publications

(43 reference statements)

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“…Clearly, whether from the morphology, phase map or bright field image, it was impossible to achieve the change of intracellular biochemical components. Previous research has demonstrated that along with the increasing of drugs treatment time, the change of nucleus condensation and fragmentation during DNR-induced Jurkat cell apoptosis can be observed by fluorescence imaging method 35 , 36 . Importantly, using our PSI/AFM system, the quantitative change of refractive index distribution during Jurkat cell apoptosis can be directly visualized, as shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Quantitative refractive index distribution of single cell by combining phase-shifting interferometry and AFM imaging

Zhang

Zhong

Tang

et al. 2017

Sci Rep

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Cell refractive index, an intrinsic optical parameter, is closely correlated with the intracellular mass and concentration. By combining optical phase-shifting interferometry (PSI) and atomic force microscope (AFM) imaging, we constructed a label free, non-invasive and quantitative refractive index of single cell measurement system, in which the accurate phase map of single cell was retrieved with PSI technique and the cell morphology with nanoscale resolution was achieved with AFM imaging. Based on the proposed AFM/PSI system, we achieved quantitative refractive index distributions of single red blood cell and Jurkat cell, respectively. Further, the quantitative change of refractive index distribution during Daunorubicin (DNR)-induced Jurkat cell apoptosis was presented, and then the content changes of intracellular biochemical components were achieved. Importantly, these results were consistent with Raman spectral analysis, indicating that the proposed PSI/AFM based refractive index system is likely to become a useful tool for intracellular biochemical components analysis measurement, and this will facilitate its application for revealing cell structure and pathological state from a new perspective.

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Section: Resultsmentioning

confidence: 99%

Quantitative refractive index distribution of single cell by combining phase-shifting interferometry and AFM imaging

Zhang

Zhong

Tang

et al. 2017

Sci Rep

Self Cite

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“…More than 100 different compounds have been isolated from Tripterygium wilfordii , and research aimed at understanding their actions is underway. One such study suggests that leukemic T-lymphocyte apoptosis is induced by triptolide, a diterpenoid epoxide, and is related to cell cycle G1 phase arrest (Zhang et al, 2015). XUEBIJING (XBJ) injection is commonly-used to treat sepsis and septic shock.…”

Section: Huoxue Tongluo (Formulas and Herbs That Promote Blood Circulmentioning

confidence: 99%

Chinese Herbal Medicine and Its Regulatory Effects on Tumor Related T Cells

Hoffman

et al. 2020

Front. Pharmacol.

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Traditional Chinese medicine is an accepted and integral part of clinical cancer management alongside Western medicine in China. However, historically TCM physicians were unaware of the chemical constituents of their formulations, and the specific biological targets in the body. Through HPLC, flow cytometry, and other processes, researchers now have a much clearer picture of how herbal medicine works in conjunction with the immune system in cancer therapy. Among them, the regulation of tumor-related T cells plays the most important role in modulating tumor immunity by traditional Chinese medicine. Encouraging results have been well-documented, including an increase in T cell production along with their associated cytokines, enhanced regulation of Tregs and important T cell ratios, the formation and function of Tregs in tumor microenvironments, and the promotion of the number and function of normal T Cells to reduce conventional cancer therapy side effects. Chinese herbal medicine represents a rich field of research from which to draw further inspiration for future studies. While promising agents have already been identified, the vast majority of Chinese herbal mechanisms remain undiscovered. In this review, we summarize the effects and mechanisms of specific Chinese herbs and herbal decoctions on tumor related T cells.

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“…Picos utilizados Atribuição na leucemia Plouvier, Huong, 1984 Entre 240 e 300 cm-1 A análise dos espectros sugere estados de oxidação na leucemia Chan et al, 2008Chan et al, 678, 785, 1093Chan et al, , 1126Chan et al, , 1337Chan et al, , 1373Chan et al, , 1447Chan et al, , 1575Chan et al, , 1605Chan et al, , 1615 Diagnóstico de leucemia linfocítica crônica em esfregaços de sangue não corados Khetani et al, 2015Khetani et al, 1032Khetani et al, , 1318Khetani et al, , 650, 789, 1003Khetani et al, , 1032Khetani et al, , 1093Khetani et al, , 1119Khetani et al, , 1283Khetani et al, , 1318Khetani et al, , 1436 Diferenciação sensível para monitorar e detectar células de leucemia e diferenciação de células apoptóticas, vivas e necróticas Vanna et al, 2015Vanna et al, 665, 755, 1249Vanna et al, , 1173Vanna et al, , 1249Vanna et al, , 1366Vanna et al, , 1397Vanna et al, , 1540Vanna et al, , 1610 Identificação de células típicas de leucemia mieloide aguda e síndrome mielodisplásica Zhang et al, 2015Zhang et al, 728, 786, 827, 1003Zhang et al, , 1102Zhang et al, , 1257Zhang et al, , 1340Zhang et al, , 1450Zhang et al, , 1660 Espectro Raman revelou a parada do ciclo celular de apoptose de linfócitos T leucêmicos induzida por triptólidos Fazio et al, 2016 1450 cm -1 Analisadas para abordar os efeitos, incluindo necrose e apoptose, induzidos pelo estresse do envelhecimento Managò et al, 2016Managò et al, 700-800, 1120Managò et al, , 1370Managò et al, , 1577 Diagnóstico, classificação e acompanhamento de leucemia linfoblástica aguda de células B Hassoun et al, 2017…”

Section: Autor/anounclassified

Diagnóstico, classificação e monitoramento de leucemias baseado em espectroscopia Raman

Lima

Silva

Daniel

2021

RSD

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O diagnóstico, classificação e monitoramento de leucemia requerem o uso e a combinação de várias tecnologias geralmente envolvendo coloração e exame da morfologia das células em uma amostra de sangue ou detecção seletiva de antígenos específicos da membrana celular. A espectroscopia Raman é uma técnica óptica baseada no espalhamento inelástico de luz por moléculas e pode fornecer informações bioquímicas altamente específicas com mínimo ou nenhum pré-tratamento de amostra. Com base nisso o presente estudo teve como objetivo realizar uma revisão sistemática por meio do levantamento de picos e marcadores em estudos experimentais e clínicos acerca do emprego da espectroscopia Raman no diagnóstico e a classificação da leucemia. Com a análise dos estudos selecionados foi possível evidenciar um grande progresso nas pesquisas sobre a aplicabilidade da espectroscopia Raman no diagnóstico, em especial sobre a sua especificidade e sensibilidade, para garantir a diferenciação entre os quatro principais subtipos de leucemias: leucemia linfoide crônica (LLC), leucemia linfoide aguda (LLA), leucemia mieloide crônica (LMC) e leucemia mieloide aguda (LMA).

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Raman spectrum reveals the cell cycle arrest of Triptolide-induced leukemic T-lymphocytes apoptosis

Cited by 17 publications

References 34 publications

Quantitative refractive index distribution of single cell by combining phase-shifting interferometry and AFM imaging

Quantitative refractive index distribution of single cell by combining phase-shifting interferometry and AFM imaging

Chinese Herbal Medicine and Its Regulatory Effects on Tumor Related T Cells

Diagnóstico, classificação e monitoramento de leucemias baseado em espectroscopia Raman

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