Raman spectroscopy of dried synovial fluid droplets as a rapid diagnostic for knee joint damage

Esmonde-White, Karen A.; Mandair, Gurjit S.; Raaii, Farhang; Roessler, Blake J.; Morris, Michael D.

doi:10.1117/12.770360

Cited by 11 publications

(8 citation statements)

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“…Previous studies of DDRS to examine solutions of proteins or biofluids found drop deposition spectra of proteins were similar to solution state or solid state spectra. (41,43,44) As expected, SF volume increased with OA damage, but the differences in SF volume were not statistically significant.…”

Section: Drop Deposition/raman Spectroscopy Of Dried Sf Dropssupporting

confidence: 60%

“…(38,39) A combined drop deposition/Raman technique for tears and synovial fluid showed subtle differences in the chemical composition at various locations in a drop. (40,41) Raman signal of poorly scattering components can be improved because impurities that fluoresce, and otherwise dominate the Raman spectra, are segregated in the drop center during the drying process. (42) Multiple studies that compared solution and dried drop Raman spectra of proteins found that solution-state conformation of proteins is preserved during the deposition process.…”

Section: Introductionmentioning

confidence: 99%

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Raman spectroscopy of synovial fluid as a tool for diagnosing osteoarthritis

et al. 2009

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For many years, viscosity has been the primary method used by researchers in rheumatology to assess the physiochemical properties of synovial fluid in both normal and osteoarthritic patients. However, progress has been limited by the lack of methods that provide multiple layers of information, use small sample volumes, and are rapid. In this blinded study, Raman spectroscopy was used to assess the biochemical composition of synovial fluid collected from forty patients with clinical evidence of knee osteoarthritis at the time of elective surgical treatment. Severity of knee osteoarthritis was assessed by a radiologist using Kellgren/Lawrence (K/L) scores from knee joint x-rays, while light microscopy and Raman spectroscopy were used to examine synovial fluid aspirates (2–10 µL), deposited on fused silica slides. We show that Raman bands used to describe protein secondary structure and content can be used to detect changes in synovial fluid from osteoarthritic patients. Several Raman band intensity ratios increased significantly in spectra collected from synovial fluid in patients with radiological evidence of osteoarthritis damage. These ratios can be used to provide a “yes/no” damage assessment. Additionally, two ratios increased with K/L score and showed moderate correlative trends. These studies provide evidence that Raman spectroscopy would be a suitable candidate in the evaluation of joint damage in knee osteoarthritis patients.

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Section: Drop Deposition/raman Spectroscopy Of Dried Sf Dropssupporting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Raman spectroscopy of synovial fluid as a tool for diagnosing osteoarthritis

et al. 2009

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“…Some other applications have come up too; patterns on dried droplets may be used for quality analysis of food grains [275], as well as alcoholic drinks [276], fast identification of fluid and substrate chemistry based on automatic pattern recognition of stains [183], assessment of quality of products [275], and Raman spectroscopy [180,[277][278][279][280][281][282][283][284][285][286].…”

Section: Biomedical Applicationmentioning

confidence: 99%

Droplet Drying Patterns on Solid Substrates: From Hydrophilic to Superhydrophobic Contact to Levitating Drops

Tarafdar

Tarasevich

Choudhury

et al. 2018

Advances in Condensed Matter Physics

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This review is devoted to the simple process of drying a multicomponent droplet of a complex fluid which may contain salt or other inclusions. These processes provide a fascinating subject for study. The explanation of the rich variety of patterns formed is not only an academic challenge but also a problem of practical importance, as applications are growing in medical diagnosis and improvement of coating/printing technology. The fundamental scientific problem is the study of the mechanism of microand nanoparticle self-organization in open systems. The specific fundamental problems to be solved, related to this system, are the investigation of the mass transfer processes, the formation and evolution of phase fronts, and the identification of mechanisms of pattern formation. The drops of liquid containing dissolved substances and suspended particles are assumed to be drying on a horizontal solid insoluble smooth substrate. The chemical composition and macroscopic properties of the complex fluid, the concentration and nature of the salt, the surface energy of the substrate, and the interaction between the fluid and substrate which determines the wetting all affect the final morphology of the dried film. The range of our study encompasses the fully wetting case with zero contact angle between the fluid and substrate to the case where the drop is levitated in space, so there is no contact with a substrate and angle of contact can be considered as 180 ∘ .

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“…One possible explanation is that the more soluble and the light-weight protein species may precipitate in the droplet center. 18 This suggests that spectra collected from the droplet edge were composed primarily of protein and amino acids macromolecules Raman bands. This may provide sufficient data for evaluating the physiochemical composition of ECM, although the coarse separation does not prevent the proteins precipitating in the center region.…”

Section: Methodsmentioning

confidence: 99%

Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy

Huang

Sun²,

Wang

et al. 2013

J. Biomed. Opt

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Abstract. In this study, a rapid and simple method which combines drop coating deposition and Raman spectroscopy (DCDR) was developed to characterize the dry embryo culture media (ECM) droplet. We demonstrated that Raman spectra obtained from the droplet edge presented useful and characteristic signatures for protein and amino acids assessment. Using a different analytical method, scanning electron microscopy coupled with energy dispersive X-ray analysis, we further confirmed that Na, K, and Cl were mainly detected in the central area of the dry ECM droplet while sulphur, an indicative of the presence of macromolecules such as proteins, was mainly found at the periphery of the droplet. In addition, to reduce sample preparation time, different temperatures for drying the droplets were tested. The results showed that drying temperature at 50°C can effectively reduce the sample preparation time to 6 min (as compared to 50 min for drying at room temperature, ∼25°C) without inducing thermal damage to the proteins. This work demonstrated that DCDR has potential for rapid and reliable metabolomic profiling of ECM in clinical applications. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Raman spectroscopy of dried synovial fluid droplets as a rapid diagnostic for knee joint damage

Cited by 11 publications

References 0 publications

Raman spectroscopy of synovial fluid as a tool for diagnosing osteoarthritis

Raman spectroscopy of synovial fluid as a tool for diagnosing osteoarthritis

Droplet Drying Patterns on Solid Substrates: From Hydrophilic to Superhydrophobic Contact to Levitating Drops

Rapid and nondestructive method for evaluation of embryo culture media using drop coating deposition Raman spectroscopy

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