Raman spectroscopy for glutathione measurements in Mycobacterium tuberculosis strains with different antibiotic resistance

Abstract

The Mycobacterium tuberculosis (MbT) drug resistance remains a serious problem to global public health and usually leads to a lethal outcome in patients with such strain MbT. Precise and rapid identification of a specific MbT strain is vital for the appointment of anti‐tuberculosis therapy. We continue investigation of deactivated MbT strains of the Beijing family by Raman spectroscopy method and focus on glutathione (GSH) as a potential biomarker for strains identification. Strains under the study have different drug sensitivity: sensitive, multi, and extensively drug resistant. Samples were investigated by Raman spectrometry with He‐Ne (633 nm) laser excitation source in the “fingerprint” region. As a result, GSH vibrational bands for potential strain discrimination were determined. These differences can be described for drug‐sensitive and drug‐resistant strains taken of pulmonary and extra‐pulmonary forms of tuberculosis. We suppose that the obtained results can be useful for new diagnostic tools development for identification of different MbT strains in clinical medicine and research practice.

Accessing Properties of Molecular Compounds Involved in Cellular Metabolic Processes with Electron Paramagnetic Resonance, Raman Spectroscopy, and Differential Scanning Calorimetry

“…This supports the importance of characterising the specificity of the lipid-based constituents of the cell envelope by different methods, including those which are considered in the present review. As another promising target, glutathione was revealed [133] in the region of moderate wave numbers (1000-1500 cm −1 ). Figure 4 highlights one of the spectral lines belonging to the glutathione spectrum, which is a rather stable representative of this molecule under various conformations and external conditions [134,135] and, simultaneously, clearly exhibit the regular amplitude growth when M. tuberculosis has extra drug resistivity.…”

The Cascade Hilbert-Zero Decomposition: A Novel Method for Peaks Resolution and Its Application to Raman Spectra

“…found by the proposed method within three iterations. The signal was normalized to unity instead of 100 used in the work [41] for the sake of convenience. Here, we used the numerical factor 6 for comparison of the interval of local fitting and the obtained dispersion of the Gaussian functions (see the description of step (III) above) for rejecting suspiciously too wide peaks.…”

Spectral and time-resolved photoluminescence of human platelets doped with platinum nanoparticles

“…due to their unique properties [ 18 – 21 ]. Localized surface plasmon resonances (LSPR), induced near the metal NPs is considered to be a powerful tool in plasmon-enhanced steady-state [ 22 ] and time-resolved [ 23 , 24 ] spectroscopy techniques applied for organic and inorganic media investigation. Plasmonic materials such as silver, gold and copper are well studied and these metals are mostly used for platelet investigations in the visible and near infrared (IR) range [ 25 , 26 ].…”