Raman Spectroscopy for Assessment of Hard Dental Tissues in Periodontitis Treatment

Timchenko, E. V.; Bazhutova, I. V.; Frolov, O. O.; Волова, Л. Т.; Тимченко, П. Е.

doi:10.3390/diagnostics11091595

“…[ 40 , 199 ] When Raman spectroscopy is used to analyze enamel, the Amide I, II, and III bond is more of a characteristic peak of enamel matrix protein. [ 58 , 200 , 201 , 202 ] A typical example is the markedly enhanced intensity of the Amide III in enamel after MMP20 knockdown, indicating that the absence of MMP20 expression results in enamel matrix proteins being blocked in the enamel. [ 154 ]…”

Section: Biomineralization Mechanismmentioning

confidence: 99%

Physicochemical understanding of biomineralization by molecular vibrational spectroscopy: From mechanism to nature

Liu

¹

,

Jiang

²

,

Liu

³

et al. 2023

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The process and mechanism of biomineralization and relevant physicochemical properties of mineral crystals are remarkably sophisticated multidisciplinary fields that include biology, chemistry, physics, and materials science. The components of the organic matter, structural construction of minerals, and related mechanical interaction, etc., could help to reveal the unique nature of the special mineralization process. Herein, the paper provides an overview of the biomineralization process from the perspective of molecular vibrational spectroscopy, including the physicochemical properties of biomineralized tissues, from physiological to applied mineralization. These physicochemical characteristics closely to the hierarchical mineralization process include biological crystal defects, chemical bonding, atomic doping, structural changes, and content changes in organic matter, along with the interface between biocrystals and organic matter as well as the specific mechanical effects for hardness and toughness. Based on those observations, the special physiological properties of mineralization for enamel and bone, as well as the possible mechanism of pathological mineralization and calcification such as atherosclerosis, tumor micro mineralization, and urolithiasis are also reviewed and discussed. Indeed, the clearly defined physicochemical properties of mineral crystals could pave the way for studies on the mechanisms and applications.

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“…[40,197] When Raman spectroscopy is used to analyze enamel, the Amide I, II, and III bond is more of a characteristic peak of enamel matrix protein. [58,[198][199][200] A typical example is the markedly enhanced intensity of the Amide III in enamel after MMP20 knockdown, indicating that the absence of MMP20 expression results in enamel matrix proteins being blocked in the enamel. [145] Other minerals…”

Section: Other Proteinsmentioning

confidence: 99%

“…Enamel is more susceptible to exogenous damage from bacteria, food debris, etc., causing plaque, dental caries, periodontitis, and other diseases in the teeth. [198,210] Meanwhile, the enamel is produced under the regulation of ameloblasts, which undergo programmed apoptosis after enamel maturation. If there is faulty signaling by ameloblasts in the regulation of enamel maturation, it can lead to irreversible amelogenesis imperfect.…”

Section: Physiological Processes and Disease Diagnosis Enamel Mineral...mentioning

confidence: 99%

“…The alteration of the characteristic peaks of Raman spectra caused by this phenomenon was used by the Timchenko group to identify the effect of the treatment of periodontitis. [198] Marco Antonio diagnosed dental fluorosis by improving the classification algorithm. [215] It was found that the b-type carbonate content increased with fluorosis severity.…”

Section: Physiological Processes and Disease Diagnosis Enamel Mineral...mentioning

confidence: 99%

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Physicochemical Understanding of Biomineralization by Molecular Vibrational Spectroscopy: From Mechanism to Nature

Liu¹,

Jiang²,

Liu³

et al. 2023

Preprint

0

View full text Add to dashboard Cite

The process and mechanism of biomineralization and relevant physicochemical properties of mineral crystals are remarkably sophisticated multidisciplinary fields that include biology, chemistry, physics, and materials science. The components of the organic matter, structural construction of minerals, and related mechanical interaction, etc., could help to reveal the unique nature of the special mineralization process. Herein, the paper provides an overview of the biomineralization process from the perspective of molecular vibrational spectroscopy, including the physicochemical properties of biomineralized tissues, from physiological to applied mineralization. These physicochemical characteristics closely to the hierarchical mineralization process include biological crystal defects, chemical bonding, atomic doping, structural changes, and content changes in organic matter, along with the interface between biocrystals and organic matter as well as the specific mechanical effects for hardness and toughness. Based on those observations, the special physiological properties of mineralization for enamel and bone, as well as the possible mechanism of pathological mineralization and calcification such as atherosclerosis, tumor micro mineralization, and urolithiasis are also reviewed and discussed. Indeed, the clearly defined physicochemical properties of mineral crystals could pave the way for studies on the mechanisms and applications.

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“…The basic approach to diagnosing caries with Raman spectroscopy is connected with detecting changes in chemical composition through the difference in the spectral characteristics of the hard dental tissues [ 37 , 38 ]. This method has been applied in dental investigations as a noncontact analytical technology for in vivo chemical specification of the intact and carious dentin [ 37 , 39 , 40 , 41 ].…”

Section: Introductionmentioning

confidence: 99%

Development of a Visualisation Approach for Analysing Incipient and Clinically Unrecorded Enamel Fissure Caries Using Laser-Induced Contrast Imaging, MicroRaman Spectroscopy and Biomimetic Composites: A Pilot Study

Seredin

¹

,

Goloshchapov

²

,

Кашкаров

³

et al. 2022

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This pilot study presents a practical approach to detecting and visualising the initial forms of caries that are not clinically registered. The use of a laser-induced contrast visualisation (LICV) technique was shown to provide detection of the originating caries based on the separation of emissions from sound tissue, areas with destroyed tissue and regions of bacterial invasion. Adding microRaman spectroscopy to the measuring system enables reliable detection of the transformation of the organic–mineral component in the dental tissue and the spread of bacterial microflora in the affected region. Further laboratory and clinical studies of the comprehensive use of LICV and microRaman spectroscopy enable data extension on the application of this approach for accurate determination of the boundaries in the changed dental tissue as a result of initial caries. The obtained data has the potential to develop an effective preventive medical diagnostic approach and as a result, further personalised medical treatment can be specified.

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Raman Spectroscopy for Assessment of Hard Dental Tissues in Periodontitis Treatment

Cited by 4 publications

References 15 publications

Physicochemical understanding of biomineralization by molecular vibrational spectroscopy: From mechanism to nature

Physicochemical understanding of biomineralization by molecular vibrational spectroscopy: From mechanism to nature

Physicochemical Understanding of Biomineralization by Molecular Vibrational Spectroscopy: From Mechanism to Nature

Development of a Visualisation Approach for Analysing Incipient and Clinically Unrecorded Enamel Fissure Caries Using Laser-Induced Contrast Imaging, MicroRaman Spectroscopy and Biomimetic Composites: A Pilot Study

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