Raman spectroscopy as a potential tool for label free therapeutic drug monitoring in human serum: the case of busulfan and methotrexate

Parachalil, Drishya Rajan; Commerford, Deirdre; Bonnier, Franck; Chourpa, Igor; McIntyre, Jennifer; Byrne, Hugh J.

doi:10.1039/c9an00801b

Cited by 23 publications

(15 citation statements)

References 55 publications

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“…Typically, the set of observations is randomly divided into approximately equal size, e.g. 50% of the spectral data randomly selected as test set, while the remaining 50% is used as the training set [101]. The cross-validation process is then repeated multiple times (the folds), such that all observations are used for both training and testing, and each observation is used for testing exactly once.…”

Section: Ftirmentioning

confidence: 99%

“…Considering the more general applications of the technique to a broader range of drugs or analytes, it is notable that both the LOD and LOQ for a given set up are determined by the STD of the measurement of the control (serum or filtered serum), and the Raman scattering efficiency of the analyte. To further illustrate this point, the LOD of vitamin B12, cholesterol, urea and glucose from liquid samples for this measurement protocol were also determined using the method previously reported by Parachalil et al [101] (Table 1). Figure 4 displays a plot of LOD versus the maximum intensity per unit acquisition time, per unit concentration.…”

Section: Ftirmentioning

confidence: 99%

“…Similarly, higher prediction accuracy (RMSECV=1.14 mg/dL) was attained when PLSR analysis was performed on a reduced range for urea from patient samples over the concentration range 2.52-78.99 mg/dL, compared to the full range (RMSECV=1.73 mg/dL). This standardised, optimised methodology was also applied to determine the Limit of Detection (LOD) and Limit of Quantification (LOQ) for therapeutic drug monitoring (TDM) in human serum, using the examples of Busulfan, a cell cycle non-specific alkylating antineoplastic agent, and, Methotrexate, a chemotherapeutic agent and immune system suppressant[101].…”

mentioning

confidence: 99%

“…LOD of glucose[44], busulfan[101], methotrexate[101], cholesterol, urea[69] and vitamin B12 calculated from the PLSR prediction plot of these analytes, compared to the maximum Raman intensity of maximum peak per unit acquisition time, per unit concentration Vibrational spectroscopic techniques have attracted a lot of attention as analytical methods of choice for analysing biological samples. The promise of the techniques is based on the abilities to objectively fingerprint the biochemical profile underlying early onset of disease in cells, tissues or bodilyfluids[3,6,9].…”

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confidence: 99%

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Potential of Raman spectroscopy for the analysis of plasma/serum in the liquid state: recent advances

2020

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There is compelling evidence in the literature to support the application of Raman spectroscopy for analysis of bodily fluids in their native liquid state. Naturally, the strategies described in the literature for Raman spectroscopic analysis of liquid samples have advantages and disadvantages. Herein, recent advances in the analysis of plasma/serum in the liquid state are reviewed. The potential advantages of Raman analysis in the liquid form over the commonly employed infrared absorption analysis in the dried droplet form are initially highlighted.Improvements in measurement protocols based on inverted microscopic geometries, clinically adaptable substrates, data preprocessing and analysis, and applications for routine monitoring of patient health as well as therapeutic administration are reviewed. These advances suggest that clinical translation of Raman spectroscopy for rapid biochemical analysis can be a reality.In the future, this method will prove to be highly beneficial to clinicians for rapid screening and monitoring of analytes and drugs in the biological fluids, and to the patients themselves, enabling early treatment, before the disease becomes symptomatic, allowing early recovery.

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Section: Ftirmentioning

confidence: 99%

Section: Ftirmentioning

confidence: 99%

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confidence: 99%

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Potential of Raman spectroscopy for the analysis of plasma/serum in the liquid state: recent advances

2020

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“…To reduce the subsequent false identification, there is a need to use a sensitive and robust technique coupled with HPLC [ 3 , 4 , 5 ]. Out of other vibrational spectroscopies, surface enhanced Raman spectroscopy (SERS) has been demonstrated in recent years for the therapeutic drug monitoring (TDM) of various drugs as well as their metabolites in biological samples [ 6 ]. SERS has many advantages such as high sensitivity, non-destructive nature, capacity to provide molecular fingerprint identification of the analyte, the capacity to measure analytes in biological fluids [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Gold-Deposited Nickel Foam as Recyclable Plasmonic Sensor for Therapeutic Drug Monitoring in Blood by Surface-Enhanced Raman Spectroscopy

et al. 2020

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A sensitive and recyclable plasmonic nickel foam sensor has been developed for surface-enhanced Raman spectroscopy (SERS). A simple electrochemical method was used to deposit flower-shaped gold nanostructures onto nickel foam substrate. The high packing of the gold nanoflowers onto the nickel foam led to a high enhancement factor (EF) of 1.6 × 1011. The new SERS sensor was utilized for the direct determination of the broad-spectrum β-lactam carbapenem antibiotic meropenem in human blood plasma down to one pM. The sensor was also used in High Performance Liquid Chromatography (HPLC)-SERS assembly to provide fingerprint identification of meropenem in human blood plasma. Moreover, the SERS measurements were reproducible in aqueous solution and human blood plasma (RSD = 5.5%) and (RSD = 2.86%), respectively at 200 µg/mL (n = 3), and successfully recycled using a simple method, and hence, used for the repeated determination of the drug by SERS. Therefore, the new sensor has a strong potential to be applied for the therapeutic drug monitoring of meropenem at points of care and intensive care units.

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Bioinspired Polyoxomolybdate Nanoclusters Peroxidase‐Mimicking Nanozyme for Dual‐Mode Detection of Methotrexate in Real Samples

Azarian,

Dehghan,

Amini

2024

Applied Organom Chemis

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Methotrexate (MTX) is a chemotherapy agent and immune system suppressant that is widely used to treat a variety of malignant and nonmalignant diseases. Due to some drawbacks of the existing methods for the determination of MTX in aquatic and real samples, we prepared a new, simple, and selective dual‐mode fluorometric and electrochemical approach based on peroxidase‐mimic [Mo36] polyoxomolybdate nanoclusters (POMo) for sensing MTX. A turn‐on–off fluorometric approach for the detection of MTX was developed, which was based on the inhibition of the emission intensity of the terephthalic acid (TA)‐H2O2 system upon the addition of MTX to the reaction solution. The electrocatalytic performance of the obtained POMo was studied after making a carbon paste electrode (CPE) utilizing cyclic voltammetry and amperometry analysis. Under the optimum condition, good linearity was observed between the quenched emission intensity of the system and MTX dosage in the range of 0.5–250 μM with a limit of detection (LOD) of 0.91 μM. The electrochemical method illustrated a much better response with an extensive linear range of 0.01–475 μM and a lower LOD (1.61 nM). Analytical recoveries and RSD% values were, respectively, in the range of 87.72%–110% and 0.33%–1.32% for the fluorometric system and 96%–106% and 0.77%–1.02% for the electrochemical platform. Finally, the selectivity of the sensor was evaluated against various common interfering species, and the results indicated satisfactory selectivity of the POMo dual‐mode system towards MTX.

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Raman spectroscopy as a potential tool for label free therapeutic drug monitoring in human serum: the case of busulfan and methotrexate

Abstract: A methodology is proposed, based on Raman spectroscopy coupled with multivariate analysis, to determine the Limit of Detection (LOD) and Limit of Quantification (LOQ) for therapeutic drug monitoring in human serum, using the examples of Busulfan and Methotrexate.

Cited by 23 publications

References 55 publications

Potential of Raman spectroscopy for the analysis of plasma/serum in the liquid state: recent advances

Potential of Raman spectroscopy for the analysis of plasma/serum in the liquid state: recent advances

Gold-Deposited Nickel Foam as Recyclable Plasmonic Sensor for Therapeutic Drug Monitoring in Blood by Surface-Enhanced Raman Spectroscopy

Bioinspired Polyoxomolybdate Nanoclusters Peroxidase‐Mimicking Nanozyme for Dual‐Mode Detection of Methotrexate in Real Samples

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