Raman spectroscopy and imaging of β-carotene in live corpus luteum cells

Arıkan, Şenay; Sands, H. S.; Rodway, R. G.; Batchelder, D. N.

doi:10.1016/s0378-4320(02)00020-9

Cited by 31 publications

(29 citation statements)

References 23 publications

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“…the spectra of different types of subcellar structures revealed that A. Kaczor and m. Pilarczyk β-carotene is present in all organelles of corpus luteum. As expected, the highest concentration of β-carotene was found in lipid fractions due to the fact that carotenoids are soluble in the lipid droplets in the cell, while nuclei and mitochondria, i. e. hydrophilic cellular compartments, are regions of the lower concentration of carotenoids [41].…”

Section: Carotenoids In Higher Plant and Single Animal Cellssupporting

confidence: 73%

“…It was previously demonstrated that the bovine corpus luteum has the highest concentration of β-carotene relative to other tissues [41]. the spectra of different types of subcellar structures revealed that A. Kaczor and m. Pilarczyk β-carotene is present in all organelles of corpus luteum.…”

Section: Carotenoids In Higher Plant and Single Animal Cellsmentioning

confidence: 94%

“…Raman spectroscopy was used to investigate of this carotenoid both in plant [39,40] and animal cells of several different systems e.g. in bovine corpus luteum cells [41,42] and oocytes of frogs [43]. this section is a brief overview of these purely experimental works.…”

Section: Carotenoids In Higher Plant and Single Animal Cellsmentioning

confidence: 99%

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Structural and Spatial Analysis of Carotenoids in a Single Cell Monitored by Raman Spectroscopy

Kaczor

Pilarczyk

2013

Challenges and Advances in Computational Chemistry and Physics

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Carotenoids are particularly convenient subjects to study by means of Raman spectroscopy due to very high Raman scattering cross-section associated with their chain vibrations. Raman studies of carotenoids in single cells have a variety of applications starting from monitoring of growth and morphogenesis of unicellular algae and ending with differentiation of cancerous versus non-cancerous tissues in humans. Examples illustrating the potential of Raman spectroscopy to investigate carotenoids structure and distribution with a particular impact on studies using chemometric and computational methods as a tool to analyze experimental data are reviewed in this chapter.

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Section: Carotenoids In Higher Plant and Single Animal Cellssupporting

confidence: 73%

Section: Carotenoids In Higher Plant and Single Animal Cellsmentioning

confidence: 94%

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Structural and Spatial Analysis of Carotenoids in a Single Cell Monitored by Raman Spectroscopy

Kaczor

Pilarczyk

2013

Challenges and Advances in Computational Chemistry and Physics

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“…In bovine luteal cells b-carotene could be detected throughout the volume of the cell, but was predominantly localized in lipid-rich cell components such as the microsomal fraction [20]. It is known that cytoplasm contains less free water than the nucleus, and this might lead to increased accumulation of hydrophobic compounds, like carotenoids, in the cytoplasm.…”

Section: Discussionmentioning

confidence: 99%

Visualization of astaxanthin localization in HT29 human colon adenocarcinoma cells by combined confocal resonance Raman and fluorescence microspectroscopy

Briviba¹,

Bornemann

Lemmer

2006

Molecular Nutrition Food Res

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Astaxanthin, a carotenoid found in plants and seafood, exhibits antiproliferative, antioxidant and anticarcinogenic properties. We show that astaxanthin delivered with tetrahydrofuran is effectively taken up by cultured colon adenocarcinoma cells and is localized mostly in the cytoplasm as detected by confocal resonance Raman and broad-band fluorescence microspectroscopy image analysis. Cells incubated with beta-carotene at the same concentration as astaxanthin (10 microM) showed about a 50-fold lower cellular amount of beta-carotene, as detected by HPLC. No detectable Raman signal of beta-carotene was found in cells, but a weak broad-band fluorescence signal of beta-carotene was observed. beta-Carotene, like astaxanthin, was localized mostly in the cytoplasm. The heterogeneity of astaxanthin and beta-carotene cellular distribution in cells of intestinal origin suggests that the possible defense against reactive molecules by carotenoids in these cells may also be heterogeneous.

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“…Raman images have even been acquired to study sub-cellular chemistry. Arikan et al [2002] have used Raman imaging as a tool to monitor beta-carotene in live corpus luteum cells, while Freeman et al [1998] have investigated the sub-cellular localization of zinc phthalocyanines, photosensitizing agents used in photodynamic therapy. Surface-enhanced Raman spectroscopy in conjunction with imaging has been used to study the chemical composition of live cells [Kneipp et al, 2002].…”

mentioning

confidence: 99%

Model-based biological Raman spectral imaging

et al. 2002

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Raman spectral imaging is a powerful tool for determining chemical information in a biological specimen. The challenge is to condense the large amount of spectral information into an easily visualized form with high information content. Researchers have applied a range of techniques, from peak-height ratios to sophisticated models, to produce interpretable Raman images. The purpose of this article is to review some of the more common imaging approaches, in particular principal components analysis, multivariate curve resolution, and Euclidean distance, as well as to present a new technique, morphological modeling. How to best extract meaningful chemical information using each imaging approach will be discussed and examples of images produced with each will be shown.

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Raman spectroscopy and imaging of β-carotene in live corpus luteum cells

Cited by 31 publications

References 23 publications

Structural and Spatial Analysis of Carotenoids in a Single Cell Monitored by Raman Spectroscopy

Structural and Spatial Analysis of Carotenoids in a Single Cell Monitored by Raman Spectroscopy

Visualization of astaxanthin localization in HT29 human colon adenocarcinoma cells by combined confocal resonance Raman and fluorescence microspectroscopy

Model-based biological Raman spectral imaging

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