Raman Spectroscopy: A Novel Experimental Approach to Evaluating Renal Tumours

Bensalah, Karim; Fleureau, Julien; Rolland, Denis; Lavastre, Olivier; Rioux‐Leclercq, Nathalie; Guilhot, François; Patard, Jean‐Jacques; Senhadji, Lotfi; Crevoisier, R. de

doi:10.1016/j.eururo.2010.06.002

Cited by 40 publications

(41 citation statements)

References 18 publications

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“…Compared to previous studies [9, 10], we first provided characteristic Raman spectrum for each type of renal tumor. These spectra could potentially serve as chemical “fingerprints” and be used for pre-operative diagnosis.…”

Section: Discussionmentioning

confidence: 99%

“…And it needs no sample pre-treatment or external labeling, and requires only a few seconds. Previous publications have presented the use of RS to detect the whole mass of renal tumor [9, 10], but no study on Raman's investigation of biopsy specimen was ever reported. In this context, we mimicked pre-operative RMB by collecting biopsy specimen from surgical removed kidney to determine whether RS is able to distinguish malignant and benign renal tumors, and serve as a complementary diagnostic tool in the future.…”

Section: Introductionmentioning

confidence: 99%

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Renal mass biopsy using Raman spectroscopy identifies malignant and benign renal tumors: potential for pre-operative diagnosis

Liu

Zhang

et al. 2017

Oncotarget

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The accuracy of renal mass biopsy to diagnose malignancy can be affected by multiple factors. Here, we investigated the feasibility of Raman spectroscopy to distinguish malignant and benign renal tumors using biopsy specimens. Samples were collected from 63 patients who received radical or partial nephrectomy, mass suspicious of cancer and distal parenchyma were obtained from resected kidney using an 18-gauge biopsy needle. Four Raman spectra were obtained for each sample, and Discriminant Analysis was applied for data analysis. A total of 383 Raman spectra were eventually gathered and each type of tumor had its characteristic spectrum. Raman could separate tumoral and normal tissues with an accuracy of 82.53%, and distinguish malignant and benign tumors with a sensitivity of 91.79% and specificity of 71.15%. It could classify low-grade and high-grade tumors with an accuracy of 86.98%. Besides, clear cell renal carcinoma was differentiated with oncocytoma and angiomyolipoma with accuracy of 100% and 89.25%, respectively. And histological subtypes of cell carcinoma were distinguished with an accuracy of 93.48%. When compared with final pathology and biopsy, Raman spectroscopy was able to correctly identify 7 of 11 “missed” biopsy diagnoses. These results suggested that Raman may serve as a promising non-invasive approach in the future for pre-operative diagnosis.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Renal mass biopsy using Raman spectroscopy identifies malignant and benign renal tumors: potential for pre-operative diagnosis

Liu

Zhang

et al. 2017

Oncotarget

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“…In kidney research, VS has been used to evaluate renal cancers, which represent almost 2% of cancers worldwide. 175 In 2010, Bensalah et al 176 applied RA-MS coupled with data supervised multivariate processing to normal renal tissues, as well as to benign and malign renal tumors of different stages and different histologic subtypes. They reported that RA-MS was able to discriminate with high accuracy (specificity and sensitivity of >80%) between (i) normal and tumor tissues, (ii) benign and malign tumors, (iii) low-grade and high-grade tumors, and 4) clear cell carcinoma, papillary tumors, and chromophobe tumors.…”

Section: Vibrational Imaging: a Powerful Emerging Toolfor Scientists mentioning

confidence: 99%

Label-free molecular imaging of the kidney

Prentice

Caprioli

Vuiblet

2017

Kidney International

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“…17 RS was also able to distinguish different subtypes of renal cell carcinoma based on its pathohistologic characteristics. 18 Crow et al 19 utilized RS to identify the androgen-dependent prostate cancer (ADPC) (LNCaP, CaP 2b) and CRPC (PC3, DU145) cells effectively. These promising results support the potential application of RS to identify CRPC in clinical practice.…”

Section: Introductionmentioning

confidence: 99%

Raman spectroscopy, a potential tool in diagnosis and prognosis of castration-resistant prostate cancer

Wang

Zeng

et al. 2013

J. Biomed. Opt

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Abstract. Purpose: We evaluated the feasibility of Raman spectroscopy (RS) in diagnosis and prognosis of castration-resistant prostate cancer (CRPC) in patients with prostate cancer (PC). Materials and methods: Raman spectra are detected from PC cell lines (LNCaP and C4-2) and tissues using a Labram HR 800 RS. Then, principal component analysis (PCA) and support vector machine (SVM) are applied for prediction. A leave-one-out cross-validation is used to train and test the SVM. Results: There are 50 qualified patients, including 33 with androgendependent prostate cancer (ADPC) and 17 with CRPC. The spectral changes at 1126, 1170, 1315 to 1338, and 1447 cm −1 between CRPC and ADPC are detected in both cells and tissues models, which are assigned to specific amino acids and DNA. PCA/SVM algorithm provided a sensitivity of 88.2% and a specificity of 87.9% for diagnosing CRPC tissues. Furthermore, 14 patients with ADPC progressed to CRPC within 12 months. These patients are separated into two groups depending on whether their cancers progressed to CRPC within 12 months. PCA/SVM could differentiate these two groups with a sensitivity of 85.7% and a specificity of 88.9%. Conclusions: RS has the potential in diagnosis and prognosis of CRPC in clinical practice. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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Raman Spectroscopy: A Novel Experimental Approach to Evaluating Renal Tumours

Cited by 40 publications

References 18 publications

Renal mass biopsy using Raman spectroscopy identifies malignant and benign renal tumors: potential for pre-operative diagnosis

Renal mass biopsy using Raman spectroscopy identifies malignant and benign renal tumors: potential for pre-operative diagnosis

Label-free molecular imaging of the kidney

Raman spectroscopy, a potential tool in diagnosis and prognosis of castration-resistant prostate cancer

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