Raman spectroscopy, a potential tool in diagnosis and prognosis of castration-resistant prostate cancer

Wang, Lei; He, Dalin; Zeng, Jin; Guan, Zhenfeng; Dang, Qiang; Wang, Xinyang; Wang, Jun; Huang, Liangwei; Cao, Peilong; Zhang, Guanjun; Hsieh, Jer‐Tsong

doi:10.1117/1.jbo.18.8.087001

Cited by 35 publications

(33 citation statements)

References 34 publications

(40 reference statements)

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“…The complete Raman frequency assignment of spectra recorded is presented in Table 1. [29][30][31][32][33][34][35][36] As a consequence of the induction of oxidative DNA fragmentation, shown in Fig. 2(B), we found major differences in the corresponding Raman spectral profiles, specifically, alterations within the 600-1700 cm À1 spectral range, which includes that band associated with the DNA phosphate backbone.…”

Section: Resultsmentioning

confidence: 89%

In vitro confocal Raman micro‐spectroscopic approach to screen the mode of action of Chinese herbs on oxidative damaged human sperm

Zhao

Yang

Chen

et al. 2016

J Raman Spectroscopy

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This paper presented a rapid and reliable approach to screen the antioxidant active ingredients on human sperm from Chinese herbs by means of confocal Raman micro-spectroscopy. For the first time with the proposed approach, we performed the studies of rationalizing the active ingredients from several Chinese herbs. In this study, the FeSO 4 /H 2 O 2 -induced oxidative damaged sperm after co-culture with compounds or active fraction extracted from some Chinese herbs commonly used in the treatment of male infertility were observed by using confocal Raman micro-spectroscopy. The Raman spectral fingerprints of the damaged sperm were very different from the normal ones, and the further statistical analysis were successfully used to support the results, these differences were shorten after co-culture with the compounds or active fraction we had chosen, and the results were almost consistent with the traditional clinical trial results and other published data, all which showed that these compounds or active fraction were the chemogenomics of the Chinese herbs on anti-oxidative damaged sperm. In brief, we demonstrated that by means of confocal Raman micro-spectroscopy, in vitro is a rapid, reliable and large-scale approach to screen the antioxidant active ingredients on human sperm from Chinese herbs.

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Section: Resultsmentioning

confidence: 89%

In vitro confocal Raman micro‐spectroscopic approach to screen the mode of action of Chinese herbs on oxidative damaged human sperm

Zhao

Yang

Chen

et al. 2016

J Raman Spectroscopy

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“…Peaks at 685, 730, 790, 1252, 1305, 1337, 1376, 1421, 1486 and 1662 cm -1 belonged to the characteristic Raman spectra of bases. And peaks at 950, 993 and 1445 cm -1 contributed to the desoxyribose and phosphodiester groups, together with the stretching vibration banding at 1052 and 1612 cm -1 [35][36][37][38][39][40][41][42] . …”

Section: Resultsmentioning

confidence: 99%

Antioxidative protective effect of icariin on the FeSO4/H2O2-damaged human sperm based on confocal raman micro-spectroscopy

Huang

Xiao

et al. 2014

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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Oxidative stress is implicated in male infertility and significantly higher reactive oxygen species are detected in 25% of infertile males. Although different agents of various alternative medicines, including traditional Chinese medicine, have been tried with varying success, evidence remains limited on whether and how much herbs or supplements might help increase the anti-oxidant ability of the sperm. This study examined the anti-oxidative effects of icariin, a flavonoid isolated from Herba Epimedii, on the human sperm. We prepared the FeSO4/H2O2-damaged human sperms, which were co-cultured with icariin in vitro, and then observed the changes of the sperm by employing Raman micro-spectroscopy. The results showed that Raman mapping with a 514 nm excitation laser allowed clear differentiation of the nucleus, neck, and, in particular, the mitochondria-rich middle piece of a human sperm cell. The effect of icariin on different organelles of the sperm was quantified by localized spectral Raman signatures obtained within milli-seconds, and icariin could keep the "Raman fingerprint" of the human sperm the same as the control groups, suggesting that icariin could protect the human sperm from being damaged by FeSO4/H2O2. Icariin may serve as a tonifying and replenishing agent of herbal origin for enhancing reproductive functions.

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“…There are many studies that have shown that PCA and Raman spectroscopy are a powerful technique in several diseases as a diagnostic tool . PCA was used to find the main principal components that maximize the variance of the most important features of Raman spectra of diabetics and healthy patients.…”

Section: Resultsmentioning

confidence: 99%

Raman spectroscopy and PCA-SVM as a non-invasive diagnostic tool to identify and classify qualitatively glycated hemoglobin levels in vivo

et al. 2016

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In this study we identify and classify high and low levels of glycated hemoglobin (HbA1c) in healthy volunteers (HV) and diabetic patients (DP). Overall, 86 subjects were evaluated. The Raman spectrum was measured in three anatomical regions of the body: index fingertip, right ear lobe, and forehead. The measurements were performed to compare the difference between the HV and DP (22 well controlled diabetic patients (WCDP) (HbA1c <6.5%), and 49 not controlled diabetic patients (NCDP) (HbA1c ≥6.5%)). Multivariable methods such as principal components analysis (PCA) combined with support vector machine (SVM) were used to develop effective diagnostic algorithms for classification among these groups. The forehead of HV versus WCDP showed the highest sensitivity (100%) and specificity (100%). Sensitivity (100%) and specificity (60%), were highest in the forehead of WCDP, versus NCDP. In HV versus NCDP, the fingertip had the highest sensitivity (100%) and specificity (80%). The efficacy of the diagnostic algorithm by receiver operating characteristic (ROC) curve was confirmed. Overall, our study demonstrated that the combination of Raman spectroscopy and PCA-SVM are feasible non-invasive diagnostic tool in diabetes to classify qualitatively high and low levels of HbA1c in vivo.

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Raman spectroscopy, a potential tool in diagnosis and prognosis of castration-resistant prostate cancer

Cited by 35 publications

References 34 publications

In vitro confocal Raman micro‐spectroscopic approach to screen the mode of action of Chinese herbs on oxidative damaged human sperm

In vitro confocal Raman micro‐spectroscopic approach to screen the mode of action of Chinese herbs on oxidative damaged human sperm

Antioxidative protective effect of icariin on the FeSO4/H2O2-damaged human sperm based on confocal raman micro-spectroscopy

Raman spectroscopy and PCA-SVM as a non-invasive diagnostic tool to identify and classify qualitatively glycated hemoglobin levels in vivo

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