Raman spectroscopic evidence for octacalcium phosphate and other transient mineral species deposited during intramembranous mineralization

Crane, Nicole J.; Popescu, Victoria; Morris, Michael D.; Steenhuis, Pieter; Ignelzi, Michael A.

doi:10.1016/j.bone.2006.02.059

Cited by 349 publications

(329 citation statements)

References 41 publications

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“…56 Our experiments also demonstrate that the lifetime of the metastable ACP and OCP phases in Considering the relevance of these results to biomineralization processes, and bone formation in particular, much less is known about the mechanism of HAP formation in vivo than in bulk solution, due to the inevitable problems associated with studying real biomineralization processes. After many decades of debate, it has finally been accepted that bone tissues can form via an ACP precursor phase, where this was determined by studying the forming cranial suture of a mouse 57 and the continuously forming bony fin rays of zebrafish. 58,59 That OCP can act as an intermediate between ACP and HAP during bone formation has also been widely discussed, but remains controversial.…”

Section: Discussionmentioning

confidence: 99%

Confinement Increases the Lifetimes of Hydroxyapatite Precursors

2014

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The mineral component of bone is a carbonated, non-stoichiometric hydroxyapatite (calcium phosphate) that forms in nanometer confinement within collagen fibrils, the principal organic constituent of bone. We here employ a model system to study the effect of confinement on hydroxyapatite precipitation from solution under physiological conditions. In common with earlier studies of calcium carbonate and calcium sulfate precipitation, we find that confinement significantly prolongs the lifetime of metastable phases, here amorphous calcium phosphate (ACP) and octacalcium phosphate (OCP). The effect occurs at surprisingly large separations of up to 1 m, and at 0.2 m the lifetime of ACP is extended by at least an order of magnitude. The soluble additive poly(aspartic acid), which in bulk stabilizes ACP, appears to act synergistically with confinement to give a greatly enhanced stability of ACP. The reason for the extended lifetime appears to be different from that found with CaCO 3 and CaSO 4 , and underscores both the variety of mechanisms whereby 2 confinement affects the growth and transformation of solid phases, and the necessity to study a wide range of crystalline systems to build a full understanding of confinement effects. We suggest that in the case of ACP and OCP the extended lifetime of these metastable phases is chiefly due to a slower transport of ions between a dissolving metastable phase, and the more stable, growing phase. These results highlight the potential importance of confinement on biomineralisation processes.3

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Section: Discussionmentioning

confidence: 99%

Confinement Increases the Lifetimes of Hydroxyapatite Precursors

2014

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“…Octacalcium phosphate (OCP: Ca 8 H 2 (PO 4 ) 6 ·5H 2 O) has been advocated as a precursor of biological apatite in bones,5, 6 and demonstrated superior bone formation to other bone substitutes 7. An OCP and collagen composite (OCP/Col) has been developed to overcome the limitations of OCP in moldability and handling performance 8.…”

Section: Introductionmentioning

confidence: 99%

Single‐dose local administration of teriparatide with a octacalcium phosphate collagen composite enhances bone regeneration in a rodent critical‐sized calvarial defect

et al. 2017

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Octacalcium phosphate and collagen composite (OCP/Col) achieves stable bone regeneration without cell transplantation in preclinical studies. Recently, a sponsor‐initiated clinical trial was conducted to commercialize the material. The present study investigated bone regeneration by OCP/Col with the single local administration of teriparatide (parathyroid hormone 1‐34; TPTD). OCP/Col was prepared by mixing sieved granules of OCP and atelocollagen for medical use and a disk was molded. After the creation of a rodent critical‐sized calvarial defect, OCP/Col or OCP/Col with dripped TPTD solution (1.0 or 0.1 µg; OCP/Col/TPTDd1.0 or OCP/Col/TPTDd0.1) was implanted into the defect. Six defects in each group were fixed 12 weeks after implantation. Radiographic examinations indicated that radiopaque figures in defects treated with OCP/Col with TPTD (OCP/Col/TPTDd) occupied a wider range than those treated with OCP/Col. Histological results demonstrated that most of the defect in OCP/Col/TPTDd was filled with newly formed bone. A histomorphometrical examination indicated that the percentage of newly formed bone was significantly higher in the defects of OCP/Col/TPTDd 1.0 (53.6 ± 4.3%) and OCP/Col/TPTDd 0.1 (52.2 ± 7.4%) than in those of OCP/Col (40.1 ± 8.4%), whereas no significant differences were observed between OCP/Col/TPTDd1.0 and OCP/Col/TPTDd0.1. These results suggest that OCP/Col with the single local administration of TPTD enhances bone regeneration in a rodent calvarial critical‐sized bone defect. © 2017 The Authors Journal of Biomedical Materials Research Part B: Applied Biomaterials Published by Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1851–1857, 2018.

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“…Whether or not vertebrate mineralization proceeds in this manner, has been the subject of a longstanding debate (7)(8)(9)(10)(11)(12)(13)(14)(15). Mature bone mineral is a highly substituted crystalline phase of calcium phosphate called carbonated hydroxyapatite (16).…”

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confidence: 99%

Amorphous calcium phosphate is a major component of the forming fin bones of zebrafish: Indications for an amorphous precursor phase

Mahamid

Sharir

Addadi

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

501

449

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A fundamental question in biomineralization is the nature of the first-formed mineral phase. In vertebrate bone formation, this issue has been the subject of a long-standing controversy. We address this key issue using the continuously growing fin bony rays of the Tuebingen long-fin zebrafish as a model for bone mineralization. Employing high-resolution scanning and transmission electron microscopy imaging, electron diffraction, and elemental analysis, we demonstrate the presence of an abundant amorphous calcium phosphate phase in the newly formed fin bones. The extracted amorphous mineral particles crystallize with time, and mineral crystallinity increases during bone maturation. Based on these findings, we propose that this amorphous calcium phosphate phase may be a precursor phase that later transforms into the mature crystalline mineral.biomineralization ͉ fish fin

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Raman spectroscopic evidence for octacalcium phosphate and other transient mineral species deposited during intramembranous mineralization

Cited by 349 publications

References 41 publications

Confinement Increases the Lifetimes of Hydroxyapatite Precursors

Confinement Increases the Lifetimes of Hydroxyapatite Precursors

Single‐dose local administration of teriparatide with a octacalcium phosphate collagen composite enhances bone regeneration in a rodent critical‐sized calvarial defect

Amorphous calcium phosphate is a major component of the forming fin bones of zebrafish: Indications for an amorphous precursor phase

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