Raman spectroscopic analysis of paddy rice infected by three pests and diseases common in Northeast Asia

Abstract

Pests and diseases seriously affect the yield and economic benefits of growing rice, and the key to inhibiting rice’s pathogenesis is to find early identification of rice infection. The characteristic Raman spectrum of healthy leaves, and leaves infected with rice blast, paddy rice bakanae and infected by Chilo suppressalis (Walker) were tested by TriVista555CRS laser Raman spectrometer (900cm−1–1700cm−1). At the same integration time, compared with healthy plants, the Raman peak of infected plants not only changed significantly, but also the signal intensity decreased. The results show that there are clear Raman peaks at the three characteristic wavenumbers of 1002.87cm−1, 1156.5cm−1 and 1522.36cm−1. Especially in the leaves of rice blast, it was found that the degree of fungal infection affects the peak width at half height of the characteristic peak. The research shows that Raman spectroscopy provides an effective method for the early detection of rice pests and diseases which may have economic benefits.

Advanced Application of Raman Spectroscopy and Surface-Enhanced Raman Spectroscopy in Plant Disease Diagnostics: A Review

“…Yue et al measured the RS spectra of intact healthy leaves and those with three diseases. 50 Healthy plants had obvious characteristic peaks at 1002.87 cm −1 (CH 3 ), 1156.5 cm −1 (C−C), and 1522.36 cm −1 (CC), while the infected leaves had a Raman shift accompanied by a decreased signal (Figure 5B). In the leaves infected with rice blast, there was a negative correlation between the diseased severity of regions and the intensities of peaks, but the difference at 1002.87 cm −1 was small (Figure 5A,B).…”

“…Paddy rice is affected by not only paddy rice blast but also by bakanae and Chilo suppressalis (Walker). Yue et al measured the RS spectra of intact healthy leaves and those with three diseases . Healthy plants had obvious characteristic peaks at 1002.87 cm –1 (CH 3 ), 1156.5 cm –1 (C–C), and 1522.36 cm –1 (CC), while the infected leaves had a Raman shift accompanied by a decreased signal (Figure B).…”

“…Raman spectra of paddy rice leaves infected with bakanae disease (D). Reproduced with permission from ref , copyright IOPscience, 2019.…”

“…RS and SERS can identify a single disease, such as citrus HLB, watermelon bacterial fruit spot disease, and rose rosettes, and can distinguish multiple diseases, i.e. citrus HLB, canker, and HLB-blight or varieties of fungal diseases in wheat and sorghum . The degree of plant disease can be distinguished, including Foc in bananas and the developed stage of bruchids within cowpeas .…”

DNA-Based Tools to Certify Authenticity of Rice Varieties—An Overview

“…These include laser-induced breakdown spectroscopy, near-infrared spectroscopy, nuclear magnetic resonance spectroscopy and Raman spectroscopy [32]. These methods were successfully employed for both rice authentication [39][40][41][42] and detection of chemical contaminants and adulterants [43][44][45][46].…”