Raman spectral analysis of the brainstem and responses of neuroglia and cytokines in whole-body gamma-irradiated rats after administration of aminothiol-based radioprotector GL2011

Abstract

The search for an effective and non-toxic radioprotector is ongoing. We tested a novel, natural aminothiol-based radioprotector, GL2011, that was applied 30 min, 3 h or 6 h after the exposure of male albino Wistar rats to a 6.7 Gy sublethal dose of gamma radiation. The molecular signatures of radioprotection were investigated with Raman microspectroscopy of brainstem tissue samples. Morphological changes and activation of astrocytes and microglia were assessed by immunohistochemistry. Global markers of neuroinflammation were followed by ELISA to monitor blood plasma levels of proinflammatory (IL-6 and TNF-?) and antiinflammatory (IL-10) cytokines. A thirty-day follow-up determined that survival of unprotected animals was 44%. A survival increase was observed after radioprotection (75%, irrespective of the time of application). Raman spectra revealed a slightly deleterious effect of radiation on nucleic acids in surviving animals that was mitigated with the radioprotector, as GL2011 preserved the morphology of both astrocytes and microglia, with reduced microglial infiltration. Cytokine assessment revealed an immunomodulatory effect of the novel radioprotector. The overall results point out the positive effects of a single dose of GL2011 applied at different times. The molecular and cellular changes in the brainstem indicate that the radioprotector applied after radiation conferred better protection, which underlines its translation to cure radiation accidents.

Assessment of cellular and molecular changes in the rat brain after gamma radiation and radioprotection by anisomycin

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Effect of Fasciola Hepatica Invasion on Cow Productivity

“…Modern science continues to search for approaches to improve health (Karpov et al, 2021;Zavalishina et al, 2022) and increase the viability of the mammalian organism (Zavalishina, 2018b;Kočović et al, 2021). For this purpose, direct observations are carried out (Zavalishina, 2018c;Zavalishina et al, 2021), and various schemes of experimental work are set up (Vorobyeva et al, 2018;Kulikov et al, 2020).…”