Raman Spectra of Poly(adenylic acid)

Aylward, Nigel; Koenig, Jack L.

doi:10.1021/ma60017a023

Cited by 34 publications

(13 citation statements)

References 5 publications

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“…Above the melting point of the duplex the line at 1513 cm" 1 increases in relative intensity. This was first observed by Aylward & Koenig (1970) using visible excitation and on the whole there is a resemblance between the normal and RR spectra of the adenine moiety (Blazej & Peticolas, 1977). Frequency doubled dye lasers show promise for probing nucleic acid electronic transitions in the ultraviolet (Blazej & Peticolas, 1977).…”

Section: Proteins and Nucleic Acids Absorbing In The Ultraviolet Amentioning

confidence: 93%

Resonance Raman spectroscopy in biochemistry and biology

Carey

1978

Quart. Rev. Biophys.

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Resonance Raman (RR) spectroscopy provides detailed information on the vibrational and electronic properties of biochemical and biological chromophores. The analysis of RR spectra, using for example model compounds or a group frequency approach, enables us to form an accurate structural picture of the chromophore in its natural biological site. Moreover, the insight gained into the electronic states of a biological chromophore can be crucial to our understanding of its function. Thus the RR technique represents a powerful means of eliciting precise structural and electronic data from a coloured species and of focusing upon key aspects of its function. It has even been possible to obtain RR spectra from some natural chromophores in vivo, giving spectra detailed and informative enough to please a spectroscopist from a system complex enough to satisfy a biologist.

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Section: Proteins and Nucleic Acids Absorbing In The Ultraviolet Amentioning

confidence: 93%

Resonance Raman spectroscopy in biochemistry and biology

Carey

1978

Quart. Rev. Biophys.

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“…Recently, work in several laboratories has shown the existence of a Raman band at about 810-814 cm-' that is always present in ribonucleic acid structures, when these structures are in an ordered or partially ordered form (4)(5)(6). This band is plainly evident in the Raman spectrum of yeast transfer RNA shown in Fig.…”

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confidence: 90%

Determination of the Backbone Structure of Nucleic Acids and Nucleic Acid Oligomers by Laser Raman Scattering

Erfurth

Kiser

Peticolas

1972

Proc. Natl. Acad. Sci. U.S.A.

243

109

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Raman spectra of fibers of DNA that have been prepared in the A, B, and C forms are presented and compared with Raman spectra of DNA and RNA in dilute solution. It is shown that the phosphate vibrations in the region 750-850 cm-' are very sensitive to the specific conformation of the phosphate group in the backbone chain and are virtually independent of all other factors. Thus, a very simple method for the determination of the specific conformation of the backbone chain of nucleic acids, at least so far as the sugar-phosphate chain is concerned, appears available. The method is applied to short oligomers and dimers of ribonucleosides. It is found that at low temperatures, at pH 7, the phosphate group goes into the geometry of the A conformation when the stacking forces between the bases are sufficiently strong.The most reliable method for the determination of the structure of nucleic acids and polynucleotide helical chains appears to be that of x-ray diffraction (1, 2). However, this method is only applicable to nucleic acids in highly concentrated fibrous form. In general, it is not applicable to dilute nucleic acid solutions, although meaningful progress in the interpretation of x-ray scattering from fairly concentrated solutions has recently been reported (3). Thus, it would seem helpful to have available a method that could be used to obtain structural information, both on fibers and on dilute solutions, where these materials naturally occur. In this paper, we wish to report the observation of several Raman bands that arise from the vibration of the sugarphosphate backbone, in both ribonucleic acids (RNA) and deoxyribonucleic acids (DNA) whose frequencies and intensities are directly related to whether or not the material is in the A, B, or C form, as designated by the x-ray crystallographers (1, 2), and are virtually independent of all other parameters, such as the base composition, the presence or absence of the 2'-hydroxyl, etc. Furthermore, these bands can be observed in single-chain structures and oligomers, so that the geometry of the phosphate group in these substances can, under favorable circumstances, be determined.Recently, work in several laboratories has shown the existence of a Raman band at about 810-814 cm-' that is always present in ribonucleic acid structures, when these structures are in an ordered or partially ordered form (4-6). This band is plainly evident in the Raman spectrum of yeast transfer RNA shown in Fig. 1, and has also been observed in ribosomal RNA (5). Upon raising the temperature of the solution, so that the secondary structure vanishes, this band at 814 cm-' inevitably vanishes (4, 5). Since this band is completely independent of base composition and is present in all ordered ribo-structures, it may be due to the sugarphosphate diestersymmetric (4, 5) stretch or antisymmetric (6) stretch. The band at 814 cm-' is highly polarized, so that the former assignment seems somewhat more reasonable. Recent work in this laboratory (4) has shown that in aqueous solution, deox...

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“…A weak Raman line appears at 1465 cm-' in the Raman spectra of all nucleosides and nucleotides which apparently arises from the sugar residue. Some striking differences are found in the Raman spectra of AMP-5' and AMP-3' which can be attributed to the sensitivity of the vibrational patterns to the point of substitution of the sugar (27), as shown in Figs. 35 and 36.…”

Section: Nucleosides and Nucleotidesmentioning

confidence: 97%