Raman spectroscopy of biological molecules: A review

Koenig, Jack L.

doi:10.1002/pol.1972.230060102

Cited by 71 publications

(53 citation statements)

References 48 publications

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“…This technique is mostly noninvasive and nondestructive at moderate photon energies and works in vitro and in vivo under a wide range of environmental conditions. It offers detailed information about the conformation, composition and intermolecular interactions of macromolecules such as DNA and proteins [19][20][21]. The first report in the literature of a single-cell Raman analysis was in fact conducted on individual living salmon sperm cells that were investigated within the cavity of a highpower laser system [22].…”

Section: Biophotonicsmentioning

confidence: 99%

Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cells

Huser

Orme

Hollars

et al. 2009

Journal of Biophotonics

110

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Healthy human males produce sperm cells of which about 25–40% have abnormal head shapes. Increases in the percentage of sperm exhibiting aberrant sperm head morphologies have been correlated with male infertility, and biochemical studies of pooled sperm have suggested that sperm with abnormal shape may contain DNA that has not been properly repackaged by protamine during spermatid development. We have used micro‐Raman spectroscopy to obtain Raman spectra from individual human sperm cells and examined how differences in the Raman spectra of sperm chromatin correlate with cell shape. We show that Raman spectra of individual sperm cells contain vibrational marker modes that can be used to assess the efficiency of DNA‐packaging for each cell. Raman spectra obtained from sperm cells with normal shape provide evidence that DNA in these sperm is very efficiently packaged. We find, however, that the relative protein content per cell and DNA packaging efficiencies are distributed over a relatively wide range for sperm cells with both normal and abnormal shape. These findings indicate that single cell Raman spectroscopy should be a valuable tool in assessing the quality of sperm cells for in‐vitro fertilization. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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Section: Biophotonicsmentioning

confidence: 99%

Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cells

Huser

Orme

Hollars

et al. 2009

Journal of Biophotonics

110

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“…Such data are introduced here only to emphasize the need for caution in analyzing intensity information. The doublet near 1460 cm-', due to methyl group deformations, is observed in valine and poly-L-valine (9,10). It is used as an internal reference.…”

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confidence: 99%

“…The appearance of two distinctly resolved peaks in the amide I region of valinomycin suggests the existence of two kinds of amide carbonyl groups (presumably hydrogen bonded and free). It has long been known that the infrared amide I vibration frequencies are shifted downward by hydrogen bonding to the C=O groups (12), and such effects have also been observed in the Raman spectra of several proteins (9). Both hydrogen bonded and free amide groups are found in the model The split doublet at 1742 and 1767 [Courtesy of Duax et (10).…”

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confidence: 99%

Raman Spectroscopic Evidence for Two Conformations of Uncomplexed Valinomycin in the Solid State

Rothschild

Asher

Anastassakis

et al. 1973

Science

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“…by comparing a series of model compounds. For example, it was found that hydrogen bonding consistently lowered the frequency of the amide C=O stretch vibration (Richards & Thompson, 1947;Koenig, 1972). This is an additional reason for attributing the narrowing of ester C=O peaks unaccompanied by a frequency shift (Fig.…”

Section: Discussionmentioning

confidence: 99%

Raman spectroscopic study of the valinomycin-KSCN complex

Asher

Rothschild

Stanley

1974

Journal of Molecular Biology

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This paper reports the first Raman spectroscopic study of the potassium complex of the cation-specific antibiotic valinomyoin.Complete Raman spectra (140 to 3000 cm-l) of crystalline valinomycin-KSCN and its Ccl,, CHCl, and CaHSOH solutions are presented and used to probe the structure of the complex in these environments. In all oases a single, narrow peak is observed in the ester C=O stretch region (1750 to 1775 cm-l) which contrasts strongly with the broad bands observed in solutions of uncomplexed valinomyoin. This is consistent with the presence of a single conformation in which all six ester C= 0 groups co-ordinate an enclosed potassium ion. We find that although the ester C=O stretch frequencies of the complex are similar in the solid state and in non-polar solution (~1770 cm-l) they are considerably different in the presence of polar solvents (~1756 cm-l); this may indicate that the complexed potassium ion is still free to interact with nearby solvent ions (and possibly its counterion) through gaps in the hydrophobic "shield"provided by the hydrocarbon residues of valinomycin.In contrast the amide C=O frequencies of the complex (~1660 cm-l) are solvent-independent.These groups are apparently strongly hydrogen-bonded to provide a rather rigid, compact framework for the complex conformation.

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Raman spectroscopy of biological molecules: A review

Cited by 71 publications

References 48 publications

Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cells

Raman spectroscopy of DNA packaging in individual human sperm cells distinguishes normal from abnormal cells

Raman Spectroscopic Evidence for Two Conformations of Uncomplexed Valinomycin in the Solid State

Raman spectroscopic study of the valinomycin-KSCN complex

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