Ralstonia solanacearum: A new threat to potato production in Serbia

Abstract

SuMMARYA survey of ware potatoes (a total of 1127 samples) from localities in Serbia during two consecutive years resulted in detection and identification of R. solanacearum in 17 tuber samples. The monitoring detected the causal agent of bacterial wilt and brown rot of potato in three districts of Vojvodina province. In 2011, the infection by R. solanacearum was confirmed in 7 samples of ware potato tubers (varieties -Saturna, Pirol, Hermes, Panda) in West Bačka and South Bačka Districts. In 2012, the infection by R. solanacearum was confirmed in 10 potato tuber samples (Lady Claire, Desiree, Panda, Red Fantasy and Vineta varieties) from two districts: South Bačka and Central Banat. Bacterial strains obtained from positive samples were identified as R. solanacearum biovar 2 using PCR/RFLP analysis, pathogenicity test on tomato transplants, and nutritional, enzymatic and biovar determination tests. To our best knowledge, these are the only findings of R. solanacearum infection in ware potatoes in Serbia. R. solanacearum was not detected in tomato or any other host plant tested in this study. Furthermore, the bacterium was not found in any of the water samples tested, including those originating from areas in which the bacterium was found in ware potato samples.

Biological control of major pathogenic bacteria of potato by Bacillus amyloliquefaciens strains SS-12.6 and SS-38.4

“…Bacterial phytopathogens such as R. solanacearum, P. carotovorum, P. brasiliense, P. punjabense, P. versatile, and D. dianthicola were isolated from potato plants and tubers in Serbia during the past decade (Milijašević-Marčić et al, 2013;Marković et al, 2021a;Marković et al, 2021b;Loc et al, 2022;Marković et al, 2022). Due to their wide host range, spreading potential, and environmental persistence, controlling these pathogens is difficult and mainly relies on preventive measures and monitoring (Charkowski et al, 2020;Ho et al, 2020).…”

Ralstonia solanacearum as a potato pathogen in Serbia: Characterization of strains and influence on peroxidase activity in tubers

“…Considering that contaminated seed (Elphinstone, 2005; EPPO, 2018) and drainage water used for irrigation (Elsas et al, 2001) present the main sources of inoculum for R . solanacearum introduction and further spread, we can speculate that in 2011 when the bacterium was introduced in the Bačka region of Vojvodina, it was established in this area for the first time (Milijašević‐Marčić et al, 2013). Subsequently, the bacterium could be further spread by drainage water to surface canals and by irrigation to reach neighbouring potato fields in this region.…”

“…In Serbia, R . solanacearum was noticed for the first time in Vojvodina (2011) on four potato cultivars (cvs Hermes, Pirol, Saturna, and Panda) and subsequently on five cultivars in 2012 (Red Fantasy, Vineta, Lady Claire, Désirée, and Panda) (Milijašević‐Marčić et al, 2013). The present study aimed to determine R .…”

Detection and Molecular Characterization of some Egyptian Isolates of Ralstonia solanacearum by Nested-PCR and PCR-RFLP Analyses

“…Amplified PCR products were analyzed by RFLP and digested with 3 units of Fast Digest restriction enzymes (Thermo Scientific™), HaeII for digestion of PCR product of primers RS600/RS61 and AvaI, PvuII for digestion of PCR product of primers Rs20/Ra201, in a total volume 30 µL containing 10 µL of PCR product and 2.5 µL enzyme buffer. Reaction mixtures were incubated at 37°C for 30 min (Poussier et al, 1999;Milijasevic-Marcic et al, 2013). The restriction fragments were separated by electrophoresis in 2.5% agarose gel containing ethidium bromide and visualized under UV-transilluminator.…”