Raineya orbicola gen. nov., sp. nov. a slightly thermophilic bacterium of the phylum Bacteroidetes and the description of Raineyaceae fam. nov.

Abstract

Raineya orbicola gen. nov., sp. nov. a slightly thermophilic bacterium of the phylum Bacteroidetes and the description of Raineyaceae fam. nov. Alexandre Lobo-da-Cunha, 4,5 Conceição Egas 1,2 and Milton S. da Costa 1, * AbstractAn isolate, designated SPSPC-11 T , with an optimum growth temperature of about 50 C and an optimum pH for growth between 7.5 and 8.0, was recovered from a hot spring in central Portugal. Based on phylogenetic analysis of its 16S rRNA sequence, the new organism is most closely related to the species of the genus Thermonema but with a pairwise sequence similarity of <85 %. The isolate was orange-pigmented, formed non-motile long filaments and rod-shaped cells that stain Gram-negative. The organism was strictly aerobic, oxidase-positive and catalase-positive. The major fatty acids were iso-C 15:0, iso-C 15 : 0 2-OH and iso-C 17 : 0 3-OH. The major polar lipids were one aminophospholipid, two aminolipids and three unidentified lipids. Menaquinone 7 was the major respiratory quinone. The DNA G+C content of strain SPSPC-11 T was 37.6 mol% (draft genome sequence). The high quality draft genome sequence corroborated many of the phenotypic characteristics of strain SPSPC-11 T . Based on genotypic, phylogenetic, physiological and biochemical characterization we describe a new species of a novel genus represented by strain SPSPC-11 T (=CECT 9012 T =LMG 29233 T ) for which we propose the name Raineya orbicola gen. nov., sp. nov. We also describe the family Raineyaceae to accommodate this new genus and species.The vast majority of the species of the phylum Bacteroidetes have optimum growth temperatures that range from about 25 C and 45 C, while slightly thermophilic or thermophilic species are very rare. Some organisms, such as Pseudozobellia thermophila We recently isolated one strain of a slightly thermophilic organism with an optimum growth temperature of around 50 C and a maximum growth temperature of 60 C. Phylogenetic analysis of the 16S rRNA gene sequence showed that this organism represents a distinct lineage within the phylum Bacteroidetes. Based on phylogenetic, physiological and biochemical parameters, we are of the opinion that strain SPSPC-11 T represents a novel genus and species, for which we propose the name Raineya orbicola gen. nov., sp. nov. We also propose that this organism represents a new Abbreviations: CAPSO, 3-(Cyclohexylamino)-2-hydroxy-1-propanesulfonic acid; CECT, Colección Española de Cultivos Tipo; COG, clusters of orthologous groups; CRISPR, clustered regularly interspaced short palindromic repeats; G+C, guanine plus cytosine; HEPES, 4-(2-hydroxyethyl)-1-piperazinethanesulfonic acid; LMG, Laboratorium voor Microbiologie Universiteit Gent; MIGS, minimum information genome sequence; SEM, scanning electron microscopy; TAPS, N-[Tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid; TEM, transmission electron microscopy. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence of strain T are KY990922, MF125287 and MF125288, respectively. The draft genome acc...

New marine-derived indolymethyl pyrazinoquinazoline alkaloids with promising antimicrobial profiles

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