Caproicibacterium amylolyticum gen. nov., sp. nov., a novel member of the family Oscillospiraceae isolated from pit clay used for making Chinese strong aroma-type liquor

Abstract

An anaerobic, Gram-stain-positive, rod-shaped, motile and spore-forming bacterium, designated strain LBM18003T, was isolated from pit clay used for making Chinese strong aroma-type liquor. Growth occurred at 20–40 °C (optimum, 30–37 °C), pH 4.5–9.5 (optimum, pH 6.5–7.0) and in the presence of 0.0–1.0 % (w/v) sodium chloride (optimum, 0 %). The predominant fatty acids were C16:0, C14:0, C14:0 DMA and C16:0 3-OH, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, two unidentified phospholipids and nine unidentified glycolipids. Phylogenetic analysis revealed that strain LBM18003T is a novel member of the family Oscillospiraceae . The 16S rRNA gene sequence similarities of strain LBM18003T to its two most closely related species were less than 94.5 % for distinguishing genera, i.e. closely related to Caproiciproducens galactitolivorans JCM 30532T (94.1 %) and Caproicibacter fermentans DSM 107079T (93.2 %). The genome size of strain LBM18003T was 2 996 201 bp and its DNA G+C content was 48.48 mol%. Strain LBM18003T exhibited 67.8 and 68.1% pairwise-determined whole-genome average nucleotide identity values to Caproiciproducens galactitolivorans JCM 30532T and Caproicibacter fermentans DSM 107079T, respectively; and showed 62.2 and 61.0 % the average amino acid identity values to Caproiciproducens galactitolivorans JCM 30532T and Caproicibacter fermentans DSM 107079T, respectively; and demonstrated 46.1 and 41.5 % conserved genes to Caproiciproducens galactitolivorans JCM 30532T and Caproicibacter fermentans DSM 107079T, respectively. The comparisons of 16S rRNA gene and genome sequences confirmed that strain LBM18003T represented a novel genus of the family Oscillospiraceae . Based on morphological, physiological, biochemical, chemotaxonomic, genotypic and phylogenetic results, strain LBM18003T represents a novel species of a novel genus of the family Oscillospiraceae , for which the name Caproicibacterium amylolyticum gen. nov., sp. nov. is proposed. The type strain is LBM18003T (=GDMCC 1.1626T=JCM 33783T).

Caproicibacterium lactatifermentans sp. nov., isolated from pit clay used for the production of Chinese strong aroma-type liquor

“… *Data was obtained from the publication by Gu et al . [3]. †Data was obtained from the publication by Flaiz et al .…”

“…For cellular fatty acid analysis, modified mCGM (GYCGM) was used to culture strain LBM19010 T . The detailed medium composition of GYCGM was described by Gu et al [3]. The cellular fatty acid compositions were analysed by using the Sherlock Microbial Identification System (midi) with the bhibla database according to the manufacturer's instructions [39].…”

“…S3). The polar lipid pattern of strain LBM19010 T was very similar to that of strain LBM18003 T within the genus Caproicibacterium [3].…”

“…LBM19010' and 'Caproicibacterium sp. JNU-WLY1368' were assigned to the family Oscillospiraceae [3][4][5][6][7][8][9]. Caproicibacterium amylolyticum, a caproate-producing bacterium isolated from pit clay of Chinese strong aroma-type liquor fermentation system, was recently proposed to the novel genus Caproicibacterium, belonging to the family Oscillospiraceae in the class Clostridia.…”

“…Comparison of the phenotypic and genomic characteristics of strain LBM19010 T , strain JNU-WLY1368 and their closely related type strains Strains: 1, LBM19010 T ; 2, JNU-WLY1368; 3, Caproicibacterium amylolyticum LBM18003 T[3]; 4, Caproiciproducens galactitolivorans JCM 30532 T [4]; 5, Caproicibacter fermentans DSM 107079 T [5]; 6, Clostridium jeddahense DSM 27834 T [40]; 7, [Clostridium] leptum DSM 753 T [41]. +, Positive; w, weak positive; −, negative; A, acetate; B, butyrate; C, caproate; E, ethanol; L, lactate; na, data not available.…”