RAFT of sulfobetaine for modifying poly(glycidyl methacrylate) microspheres to reduce nonspecific protein adsorption

Abstract

The minimization of nonspecific protein adsorption is a crucial step in the development of bioseparation processes, immunoassays, and affinity diagnostics. Among the numerous biomaterials, polyzwitterions are known to effectively suppress protein and cell adhesion. This article describes the formation of monodisperse polymer microspheres coated with polysulfobetaine with the aim to limit nonspecific adsorption of bovine serum albumin (BSA) as a model protein. In this process, 2-lm poly(glycidyl methacrylate) (PGMA) microspheres were prepared by dispersion polymerization. To render the microspheres hydrophilic and biocompatible, [3-(methacryloylamino)propyl]dimethyl(3-sulfopropyl)ammonium hydroxide (MPDSAH) was grafted from the surface by reversible addition-fragmentation chain transfer (RAFT) polymerization. Elemental analysis of the modified microspheres revealed up to 20 wt % of poly{[3-(methacryloylamino)propyl]dimethyl(3-sulfopropyl)ammonimum hydroxide} (PMPDSAH). The microspheres were characterized in terms of particle size, morphology, and zeta potential. The amount of BSA nonspecifically adsorbed on the PMPDSAH-modified microspheres decreased to half of that captured on the unmodified PGMA microspheres.

Thiol‐epoxy “click” chemistry: Application in preparation and postpolymerization modification of polymers

“…Recently, this strategy is applied to prepare composite magnetic nanoparticles . The idea of using epoxide‐functionalized microparticles to anchor functionalities onto the particle surface is also used by Horak and coworkers in a unique manner . They anchored a reversible addition fragmentation chain transfer (RAFT) agent onto a poly(glycidyl methacrylate) particle through a thiol‐epoxy reaction.…”

Raft polymerization ofN,N-dimethylacrylamide from magnetic poly(2-hydroxyethyl methacrylate) microspheres to suppress nonspecific protein adsorption

“…). Compared with the sulfobetaine‐modified poly(glycidyl methacrylate) particles, the magnetic PHEMA‐PDMA microspheres reduced nonspecific protein adsorption much more.…”