RAF1 mediates the FSH signaling pathway as a downstream molecule to stimulate estradiol synthesis and secretion in mouse ovarian granulosa cells

Luo, Xuan; Liu, Hui; Guo, Hongzhou; Sun, Longjie; Gou, Kemian; Cui, Sheng

doi:10.21037/atm-22-393

Cited by 3 publications

(1 citation statement)

References 40 publications

(39 reference statements)

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“…V-RAF-leukemia viral oncogene 1 (RAF1) is the core member of the RAS/RAF/MEK/ERK signaling pathway and has been approved to play an essential role in reproduction and development by regulating steroid hormone synthesis ( 35 , 36 ). Through in vitro and in vivo studies, Luo et al ( 37 ) found that RAF1 could stimulate E2 synthesis and secretion through the FSH signaling pathway in mouse granulosa cells. Nonetheless, the role of RAF1 in ovarian function and follicular development of poultry has been largely understudied.…”

Section: Discussionmentioning

confidence: 99%

Identification of differentially expressed genes and signaling pathways in Gaoyou duck ovary at different physiological stages

et al. 2023

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IntroductionGaoyou duck is famous in China and abroad for its good production of double-yolk eggs. However, there has been no systematic research on the egg-laying characteristics of the Gaoyou duck, which limits the development and utilization of breed resource.MethodsTo identify the essential genes related to ovarian development, the transcriptome profiles of the ovaries of Gaoyou ducks at different physiological stages were analyzed. The transcriptome profiles of the ovaries of Gaoyou ducks at 150 d (before laying), 240 d (egg laying) and 500 d (nesting) were constructed, and the differentially expressed genes (DEGs) underwent GO (gene ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) analyses.ResultsThe 6 randomly selected DEGs were verified by real-time fluorescent quantitative PCR that their relative expression was consistent with the transcriptional expression profile. Furthermore, KEGG analysis found that 8 candidate signaling pathways were essential for ovarian development, including the MAPK signaling pathway, Progesterone-mediated oocyte maturation, Cell adhesion molecules (CAMs), NOD-like receptor signaling pathway, ECM-receptor interaction, Focal adhesion, TGF-beta signaling path-way and Phagosome. Finally, 5 key DEGs were identified to participate in ovarian development, including TGIF1, TGFBR2, RAF1, PTK2 and FGF10.DiscussionOur findings reveal the mechanisms under-lying the molecular regulation of related genes in Gaoyou duck ovarian development.

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Section: Discussionmentioning

confidence: 99%

Identification of differentially expressed genes and signaling pathways in Gaoyou duck ovary at different physiological stages

et al. 2023

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Casein kinase 1α mediates estradiol secretion via CYP19A1 expression in mouse ovarian granulosa cells

Luo,

Zhang,

Zheng

et al. 2024

BMC Biol

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Background Casein kinase 1α (CK1α), expressed in both ovarian germ and somatic cells, is involved in the initial meiosis and primordial follicle formation of mouse oocytes. Using in vitro and in vivo experiments in this study, we explored the function and mechanism of CK1α in estrogen synthesis in mice ovarian granulosa cells. Methods A CK1α knockout (cKO) mouse model, targeted specifically to ovarian granulosa cells (GCs), was employed to establish the influence of CK1α on in vivo estrogen synthesis. The influence of CK1α deficiency on GCs was determined in vivo and in vitro by immunofluorescence analysis and Western blot assay. Transcriptome profiling, differentially expressed genes and gene functional enrichment analyses, and computation protein–protein docking, were further employed to assess the CK1α pathway. Furthermore, wild-type female mice were treated with the CK1α antagonist D4476 to elucidate the CK1α's role in estrogen regulation. Results Ovarian GCs CK1α deficiency impaired fertility and superovulation of female mice; also, the average litter size and the estradiol (E2) level in the serum of cKO female mice were decreased by 57.3% and 87.4% vs. control mice, respectively. This deficiency disrupted the estrous cycle and enhanced the apoptosis in the GCs. We observed that CK1α mediated the secretion of estradiol in mouse ovarian GCs via the cytochrome P450 subfamily 19 member 1 (CYP19A1). Conclusions These findings improve the existing understanding of the regulation mechanism of female reproduction and estrogen synthesis. Trial registration Not applicable.

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Sterigmatocystin declines mouse oocyte quality by inducing ferroptosis and asymmetric division defects

Wang,

Wu,

Yang

et al. 2024

J Ovarian Res

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RAF1 mediates the FSH signaling pathway as a downstream molecule to stimulate estradiol synthesis and secretion in mouse ovarian granulosa cells

Cited by 3 publications

References 40 publications

Identification of differentially expressed genes and signaling pathways in Gaoyou duck ovary at different physiological stages

Identification of differentially expressed genes and signaling pathways in Gaoyou duck ovary at different physiological stages

Casein kinase 1α mediates estradiol secretion via CYP19A1 expression in mouse ovarian granulosa cells

Sterigmatocystin declines mouse oocyte quality by inducing ferroptosis and asymmetric division defects

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