Radiosensitizing effect of ferulic acid on human cervical carcinoma cells in vitro

Karthikeyan, S.; Kanimozhi, Govindhasamy; Prasad, Nagarajan Rajendra; Mahalakshmi, Rajendran

doi:10.1016/j.tiv.2011.05.007

Cited by 90 publications

(49 citation statements)

References 50 publications

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“…The formation of ROS was measured by using a nonfluorescent probe, 2,7-diacetyl dichlorofluorescein (DCFH-DA) that can penetrate into the intracellular matrix of cells, where it is oxidized by ROS to form fluorescent dichlorofluorescein (DCF) [16]. The percentage of ROS was estimated in the control, hesperidin, and rotenone-treated SK-N-SH neuroblastoma cells.…”

Section: Methodsmentioning

confidence: 99%

Neuroprotective Effects of Hesperidin, a Plant Flavanone, on Rotenone-Induced Oxidative Stress and Apoptosis in a Cellular Model for Parkinson’s Disease

Tamilselvam¹,

Braidy

Manivasagam³

et al. 2013

Oxidative Medicine and Cellular Longevity

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146

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Rotenone a widely used pesticide that inhibits mitochondrial complex I has been used to investigate the pathobiology of PD both in vitro and in vivo. Studies have shown that the neurotoxicity of rotenone may be related to its ability to generate reactive oxygen species (ROS), leading to neuronal apoptosis. The current study was carried out to investigate the neuroprotective effects of hesperidin, a citrus fruit flavanol, against rotenone-induced apoptosis in human neuroblastoma SK-N-SH cells. We assessed cell death, mitochondrial membrane potential, ROS generation, ATP levels, thiobarbituric acid reactive substances, reduced glutathione (GSH) levels, and the activity of catalase, superoxide dismutase (SOD) and glutathione peroxidase (GPx) using well established assays. Apoptosis was determined in normal, rotenone, and hesperidin treated cells, by measuring the protein expression of cytochrome c (cyt c), caspases 3 and 9, Bax, and Bcl-2 using the standard western blotting technique. The apoptosis in rotenone-induced SK-N-SH cells was accompanied by the loss of mitochondrial membrane potential, increased ROS generation, the depletion of GSH, enhanced activities of enzymatic antioxidants, upregulation of Bax, cyt c, and caspases 3 and 9, and downregulation of Bcl-2, which were attenuated in the presence of hesperidin. Our data suggests that hesperidin exerts its neuroprotective effect against rotenone due to its antioxidant, maintenance of mitochondrial function, and antiapoptotic properties in a neuroblastoma cell line.

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Section: Methodsmentioning

confidence: 99%

Neuroprotective Effects of Hesperidin, a Plant Flavanone, on Rotenone-Induced Oxidative Stress and Apoptosis in a Cellular Model for Parkinson’s Disease

Tamilselvam¹,

Braidy

Manivasagam³

et al. 2013

Oxidative Medicine and Cellular Longevity

Self Cite

146

View full text Add to dashboard Cite

show abstract

“…Since the increased level of copper was observed in many cancers, and cancer cells are usually under greater oxidative stress than normal cells, the pro-oxidant ability of ferulic acid might lead to selective cytotoxicity to cancer cells [157]. Ferulic acid (10 μg/mL) also decreased cell viability and enhanced efficacy of radiotherapy in two cervical cancer cell lines (HeLa and ME-180), possibly through promotion of ROS [159]. Another study on prostate cancer found that the effects of ferulic acid varied with cell types.…”

Section: Experimental Studiesmentioning

confidence: 99%

Natural Polyphenols for Prevention and Treatment of Cancer

et al. 2016

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There is much epidemiological evidence that a diet rich in fruits and vegetables could lower the risk of certain cancers. The effect has been attributed, in part, to natural polyphenols. Besides, numerous studies have demonstrated that natural polyphenols could be used for the prevention and treatment of cancer. Potential mechanisms included antioxidant, anti-inflammation as well as the modulation of multiple molecular events involved in carcinogenesis. The current review summarized the anticancer efficacy of major polyphenol classes (flavonoids, phenolic acids, lignans and stilbenes) and discussed the potential mechanisms of action, which were based on epidemiological, in vitro, in vivo and clinical studies within the past five years.

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“…Vanillic acid, a vegetable phenolic compound, is a strong antioxidant (Erdem et al, 2012). Moreover, ferulic acid enhances radiation effects by increasing lipid peroxidative markers in two cervical cancer cell lines, namely HeLa and ME-180, in vitro (Karthikeyan et al, 2011;Serafim et al, 2011). An in vivo model has shown that resveratrol has not only anti-inflammatory, anti-oxidant, anti-mutation, anti-proliferation and anticell cycle activities but also induces apoptosis and has synergistic effects with chemotherapeutic drugs (Frampton et al, 2010;Hahnvajanawong et al, 2011).…”

Section: 449 Cytotoxic Effects Of Phytophenolics From Caesalpinia MImentioning

confidence: 99%

“…Plant phytochemicals have been reported to prevent a number of diseases, including cancer, cardiovascular disease, infection and inflammation. In vitro studies have revealed that some phytochemicals, such as gallic acid (Gali et al, 1991;Ohno et al, 2001;Kim 2007;You et al, 2010;Lu et al, 2010), caffeine (Kawano et al, 2012), vanillic acid (Erdem et al, 2012), and ferulic acid (Karthikeyan et al, 2011;Serafim et al, 2011), are cytotoxic to cell lines. An in vivo model has shown that resveratrol not only has anti-inflammatory, anti-oxidative, anti-mutation, anti-proliferative, and anti-cell cycle properties but also induces apoptosis and has synergistic effects with chemotherapeutic drugs (Frampton et al, 2010;Hahnvajanawong et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Cytotoxic Effects of Phytophenolics from Caesalpinia mimosoides Lamk on Cervical Carcinoma Cell Lines through an Apoptotic Pathway

Palasap¹,

Limpaiboon²,

Boonsiri³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Radiosensitizing effect of ferulic acid on human cervical carcinoma cells in vitro

Cited by 90 publications

References 50 publications

Neuroprotective Effects of Hesperidin, a Plant Flavanone, on Rotenone-Induced Oxidative Stress and Apoptosis in a Cellular Model for Parkinson’s Disease

Neuroprotective Effects of Hesperidin, a Plant Flavanone, on Rotenone-Induced Oxidative Stress and Apoptosis in a Cellular Model for Parkinson’s Disease

Natural Polyphenols for Prevention and Treatment of Cancer

Cytotoxic Effects of Phytophenolics from Caesalpinia mimosoides Lamk on Cervical Carcinoma Cell Lines through an Apoptotic Pathway

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