Radiosensitivity of Developing Reproductive Cells in Female Cochliomyia Hominivorax

The Modification of Reproduction in Insects Treated With Alkylating Agents. I. Inhibition of Ovarian Growth and Egg Production and Hatchability

“…The next morning, 17 hours later, the females were given the opportunity to lay eggs and the rate of oviposition and percentage of hatch were determined as previously described (LaChance and Leverich, 1962)…”

Oogenesis and Radiosensitivity in Cochliomyia Hominivorax (Diptera: Calliphoridae)

“…9B and C). The oocyte has grown noticeably and is beginning to elongate ; the oocyte nucleus is located in the ooplasm very near the nurse cells and is in prophase I of the first meiotic division (LaChance and Leverich, 1962). The second egg chamber has also enlarged and contains nurse cells undergoing endomitotic replications.…”

“…9D). The disappearance of the nurse cells from the first egg chamber is correlated with the passage of the oocyte nucleus from prophase I to metaphase I of the first meiotic division (LaChance and Leverich, 1962).…”

“…Some aspects of the development of the female reproductive system in the screwworm fly, Cochliomyia hominivorax (Cqrl. ), have been reported, in connection with work on radiation-induced dominant lethal mutations in developing oocytes (LaChance and Leverich, 1962). The present studies are concerned with the inhibition of ovarian growth by radiation, and with the morphological and cytological changes that lead to sterility characterized by infecundity.…”

Induction of Dominant Lethal Mutations in Insect Oocytes and Sperm by Gamma Rays and an Alkylating Agent: Dose-Response and Joint Action Studies

“…By treating one female, hundreds of oocytes, all in the same stage of development, are simultanzously exposed to the treatment. Gravid females deposit 200 to 250 eggs in a sagle mass.Three-day-old females reared at 80°F contain oocytes with the nucleus in early prophase of the first meiotic division, 4-day-old females contain almost fully mature eggs with the nucleus in metaphas2 I, and 5-day-old females contain a large number of fully mature eggs in which meiosis has progressed up to early anaphase I (LACHANCE and LEVERICH 1962). Further growth and changes in the egg and its nucleus are arrested in this stage until the egg is laid.…”