Radioprotective Effect of Local Administration of Lysine-Vasopressin and Triglycyl-Lysine-Vasopressin on the Rectal Mucosa in Rats

Bjelkengren, Göran; Aronsen, K. F.; Augustsson, Nils E.; Borgström, S; Lindström, C; Nylander, G

doi:10.3109/02841869509094012

Cited by 3 publications

(3 citation statements)

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“…The absorbed dose rate was 3.2 Gy/minute and consequently the irradiation time for each animal was approximately 6 minutes for 19 Gy (n = 18) and 12 minutes for 38 Gy (n = 18). An absorbed dose of 19 Gy delivered to the intestine is known to cause consistent structural, cellular, and molecular changes [ 15 ]. The sham operated mice underwent the same surgical procedure except irradiation.…”

Section: Methodsmentioning

confidence: 99%

Local radiotherapy of exposed murine small bowel: Apoptosis and inflammation

et al. 2008

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BackgroundPreoperative radiotherapy of the pelvic abdomen presents with complications mostly affecting the small bowel. The aim of this study was to define the features of early radiation-induced injury on small bowel.Methods54 mice were divided into two groups (36 irradiated and 18 sham irradiated). Animals were placed on a special frame and (in the radiated group) the exteriorized segment of ileum was subjected to a single absorbed dose of 19 or 38 Gy radiation using 6 MV high energy photons. Specimens were collected for histology, immunohistochemistry (IHC) and ELISA analysis after 2, 24 and 48 hours. Venous blood was collected for systemic leucocyte count in a Burker chamber.ResultsHistology demonstrated progressive infiltration of inflammatory cells with cryptitis and increased apoptosis. MIP-2 (macrophage inflammatory protein) concentration was significantly increased in irradiated animals up to 48 hours. No significant differences were observed in IL-10 (interleukin) and TNF-α (tumour necrosis factor) levels. IHC with CD45 showed a significant increase at 2 hours of infiltrating leucocytes and lymphocytes after irradiation followed by progressive decrease with time. Caspase-3 expression increased significantly in a dose dependent trend in both irradiated groups up to 48 hours.ConclusionAcute small bowel injury caused by local irradiation is characterised by increased apoptosis of crypt epithelial cells and by lymphocyte infiltration of the underlying tissue. The severity of histological changes tends to be dose dependent and may affect the course of tissue damage.

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Section: Methodsmentioning

confidence: 99%

Local radiotherapy of exposed murine small bowel: Apoptosis and inflammation

et al. 2008

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“…Using an asymmetrically half blocked 6 MV beam and extra lead shielding (Figure 1), the treatment field perfectly fitted the exteriorised intestine while the remaining body was kept outside the radiation beam. An absorbed dose of 19 Gy was delivered to the intestine as this dose causes consistent structural, cellular, and molecular changes [11]. The absorbed dose rate was 3.2 Gy/minute and consequently the irradiation time for each animal was approximately 6 minutes.…”

Section: Methodsmentioning

confidence: 99%

Radiation enteropathy and leucocyte-endothelial cell reactions in a refined small bowel model

et al. 2004

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Background: Leucocyte recruitment and inflammation are key features of high dose radiation-induced tissue injury. The inflammatory response in the gut may be more pronounced following radiotherapy due to its high bacterial load in comparison to the response in other organs. We designed a model to enable us to study the effects of radiation on leucocyte-endothelium interactions and on intestinal microflora in the murine ileum. This model enables us to study specifically the local effects of radiation therapy.

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“…Using an asymmetrically half blocked 6 MV beam and extra lead shielding, the treatment field perfectly fitted the exteriorized intestine while the remaining body was kept outside the radiation beam. An absorbed dose of 19 or 38 Gy was delivered to the intestine as this dose causes consistent structural, cellular, and molecular changes [16]. The absorbed dose rate was 3.2 Gy/min, and consequently, the irradiation time for each animal was approximately 6 and 8 min for 19 and 38 Gy groups, respectively.…”

Section: Radiationmentioning

confidence: 99%