Radiopharmaceuticals and Fluorescein Sodium Mediated Triple‐Modality Molecular Imaging Allows Precise Image‐Guided Tumor Surgery

Abstract

Radical resection is the most effective method for malignant tumor treatments. However, conventional imaging cannot fully satisfy the clinical needs of surgical navigation. This study presents a novel triple‐modality positron emission tomography (PET)–Cerenkov radiation energy transfer (CRET)–confocal laser endomicroscopy (CLE) imaging strategy for intraoperative tumor imaging and surgical navigation. Using clinical radiopharmaceuticals and fluorescein sodium (FS), this strategy can accurately detect the tumor and guide the tumor surgery. The FS emission property under Cerenkov radiation excitation is investigated using 2‐deoxy‐2‐ 18 F‐fluoroglucose and 11 C‐choline. Performances of the PET–CRET–CLE imaging and the CRET–CLE image‐guided surgery are evaluated on mouse models. The CRET signal at 8 mm depth is stronger than the Cerenkov luminescence at 1 mm depth in phantoms. In vivo experiments indicate that 0.5 mL kg −1 of 10% FS generates the strongest CRET signal, which can be observed immediately after FS injection. A surgical navigation study shows that the tumors are precisely detected and resected using intraoperative CRET–CLE. In summary, a PET–CRET–CLE triple‐modality imaging strategy is developed. This strategy can detect the tumors and precisely guide the tumor resection using clinical pharmaceuticals. This triple‐modality imaging shows high potential in surgical navigation research and clinical translation.

Perilla frutescens Leaf Extract and Fractions: Polyphenol Composition, Antioxidant, Enzymes (α-Glucosidase, Acetylcholinesterase, and Tyrosinase) Inhibitory, Anticancer, and Antidiabetic Activities

“…Recent studies reported that diabetes could contribute to the development of cancer via inflammation, and thus has been identified as a risk factor for cancer and cancer-related mortality [ 36 , 37 ]. Malignant tumors such as hepatocellular carcinoma and breast cancer are important causes of human death, and a significant link between them and diabetes has been evidenced [ 38 , 39 , 40 , 41 ].…”

Cutting‐edge advancements of nanomaterials for medi‐translatable noninvasive theranostic modalities

“…[28][29][30] Ultrasonography (US) Ref. 17 Photodynamic therapy (PDT) Ref. [31][32][33][34][35][36][37][38][39] Magneto-acoustic imaging (MAI) Ref.…”

“…15 ) with PBCA, an FDA-approved biomaterial, which exhibited prolonged life-time and excellent stability due to the polymeric shell preventing gas core from shrinkage. 17 With the in situ one-pot polymerization, a narrowly distributed and monodispersed population of MBs could be afforded, and provide a real-time visualization for discriminating versatile processes of molecular imaging, MB disintegration, and drug release from MBs as drug payloads. Thus, it may be used for image-guided surgery and achieve high efficiency of synergistic treatments combining ultrasound and these polymeric MBs.…”

Advanced Nanotechnology Leading the Way to Multimodal Imaging‐Guided Precision Surgical Therapy

“…Cheng's team reported an α v β 3 integrin-targeted PET/NIR II nanoprobe ( 68 Ga-SCH2) for tumor delineation and targeting IGS. [236][237][238][239][240][241][242][243] compared with FGS using ICG, in subcutaneous breast cancer 4T1 tumor, orthotopic, and orthotopic-ectopic hepatocellular carcinoma tumor models. [235] CL imaging (CLI) has been successfully used for LN mapping and IGS in animal models.…”

“…[235] CL imaging (CLI) has been successfully used for LN mapping and IGS in animal models. [236] CRET can convert the ultraviolet/blue-weighted light from CL to longer wavelength. [239] Recently, the same group developed a kind of triple-modal molecular imaging combining PET, Cerenkov radiation energy transfer (CRET), and confocal laser endomicroscopy (CLE), called PET-CRET-CLE imaging.…”