Radiolysis of model polypeptides in the solid state: Radiation sensitivity and side chain structure

Hill, David J. T.; Garrett, R W; Ho, Sook-Ying; O’Donnell, James H.; O'Sullivan, Paul W.; Pomery, Peter J.

doi:10.1016/0146-5724(81)90267-3

Cited by 17 publications

(4 citation statements)

References 12 publications

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“…In carefully controlled studies, Hill et al (1981), irradiated samples in vacuo, and analyzed the volatile radioproducts by EPR and by gas chromatography without exposure to oxygen. Homopolymers of alanine, glycine, valine, phenylalanine or tyrosine were irradiated in a 60 Co source at 25 °C or -196 °C.…”

Section: Radiation Damage and The Primary Structure Of Proteinsmentioning

confidence: 99%

Damage to proteins due to the direct action of ionizing radiation

Kempner

1993

Quart. Rev. Biophys.

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Section: Radiation Damage and The Primary Structure Of Proteinsmentioning

confidence: 99%

Damage to proteins due to the direct action of ionizing radiation

Kempner

1993

Quart. Rev. Biophys.

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“…Radiolysis of model polypeptides using the homopolymer containing amino acids (Gly, Ala, Val, Phe, and Tyr) in the solid state has been reported . The predominant radiolysis product of polypeptides is the α-carbon radical (C α ) (except for polytytosine) formed by H-abstraction from carbon on the main chain of the polypeptide.…”

Section: Results and Discussionmentioning

confidence: 99%

“…Radiolysis of model polypeptides using the homopolymer containing amino acids (Gly, Ala, Val, Phe, and Tyr) in the solid state has been reported. 62 The predominant radiolysis product of polypeptides is the α-carbon radical (C α ) (except for polytytosine) formed by H-abstraction from carbon on the main chain of the polypeptide. The C α radical is the long-lived radical species, which was observed by direct electron spin resonance (ESR) measurements of the irradiated polypeptide solids.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Strengthened Silk-Fibroin/Poly(ethylene oxide) Nonwoven Nanofibers: A Dual Green Process Using Pure Water for Electrospinning and Electron Beam-Assisted Cross-Linking

Wongkrongsak

Pangon

Pongsak

et al. 2022

ACS Sustainable Chem. Eng.

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A combination process of using pure water for electrospinning and electron beam (EB)-assisted cross-linking is proposed as a dual green route to obtain strengthened silk fibroin (SF)-based nonwoven nanofibers toward value-added healthcare or biomedical products. The first challenge is to optimize the electrospinning process using SF/poly(ethylene oxide) (PEO) in a pure water solution for the fabrication of nonwoven nanofibers. Another technological challenge is to stabilize the dissoluble SF/ PEO nonwoven nanofibers through a cross-linking network created by a free radical reaction using EB irradiation. Herein, we optimized SF/PEO combination and the electrospinning parameters for the fabrication of SF-PEO nanofibers. Under mild conditions using an aqueous solution of SF-PEO and tunable electrospinning parameters, elongated, cylindrical-shaped, and smooth SF-PEO nanofibers with a diameter of 169 ± 5 nm were obtained. The stability of the SF-PEO nanofibers could be tuned by EB irradiation with the absorbed doses in the range of 10−100 kGy. Aiming at well-defined characteristics of nanofibers, the morphology, gel fraction, kinetics of swelling behavior, chemical and packing structure, thermal and mechanical properties, cell cytotoxicity, and proliferation were characterized and relatively clarified with the influencing parameters. It is important to note that EB irradiation with a reliable sterilization dose of 25 kGy was an optimum dose for cross-linking. Upon cross-linking the SF-PEO nanofibers, the dissolution of the nanofibers reduced, they became more stable, and their mechanical properties significantly improved with an increase in ductility by ∼22-fold. The cross-linked SF-PEO nanofibers promoted cell proliferation and proved nontoxic to skin fibroblast cells (L929), and they are thus appropriate for healthcare and biomedical applications. This study shows the potential of using 3G approaches, i.e., green renewable polymers, green electrospinning, and a green EB irradiation process, for sustainable chemistry for biomedical engineering applications.

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“…The great majority of amino-acids (histidine, lysine, proline, tyrosine, fects observed by other techniques such as microelectrophoresis or electronic microscopy [21]. Thus the fundamental studies [22][23][24][25] must be reoriented to find what would be the life time of the ESR signals, product per product: the presence of an EPR signal will be a proof of irradiation but its absence will not be a proof of no irradiation, at least for some amino acids.…”

Section: Esr Study Of Gamma Induced Radicals In Drugs and Excipientsmentioning

confidence: 99%

Electron paramagnetic resonance of radicals induced in drugs and excipients by radiation or mechanical treatments

Raffi¹,

Gelly²,

Barral³

et al. 2002

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Radiation as well as mechanical treatments induced in drugs and excipients radicals, which can be studied by electron paramagnetic resonance. A special attention is pointed about the use of electron paramagnetic resonance (EPR) to bring the proof whether or not a drug has been irradiated or not. We also discuss of other methods (thermoluminescence (TL), gas phase chromatography (GPC)) which can be used to bring the same proof in case of irradiated drugs, excipients and cosmetic products.

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Radiolysis of model polypeptides in the solid state: Radiation sensitivity and side chain structure

Cited by 17 publications

References 12 publications

Damage to proteins due to the direct action of ionizing radiation

Damage to proteins due to the direct action of ionizing radiation

Strengthened Silk-Fibroin/Poly(ethylene oxide) Nonwoven Nanofibers: A Dual Green Process Using Pure Water for Electrospinning and Electron Beam-Assisted Cross-Linking

Electron paramagnetic resonance of radicals induced in drugs and excipients by radiation or mechanical treatments

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