Abstract. This work is based on the describing a new approach in estimation of Concentration Factor (CF) for marine organisms radionuclides uptake. We considered the dependence of CF from the water salinity (determining the K + and Ca 2+ ions). The radionuclide uptake by the marine biota of Dnieper-Bug Estuary using the numerical compartment-model POSEIDON-R with latest modifications was applied. The levels of radionuclides in the different marine organisms, representing the various types of marine organisms in the different trophic levels of the foodweb, were calculated. The uptake model takes into account both the uptake of radionuclides via food consumption, by describing the predator-prey relations, and the direct uptake of radionuclides from the water via the gills.