Radiolabelling by tritium and [<sup>125</sup>I]iodine of an angiotensin II related Peptide

Pham, Paul; Ramombordes, C.; Perret, C.; Ronco, P.; Budisavljevic, Milos N.; Verroust, P; Beaucourt, J. P.

doi:10.1002/jlcr.2580290509

Cited by 3 publications

(2 citation statements)

References 6 publications

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“…Iodination Radio-iodination ( I) of proANP(l-30) was performed by the chloramine method according to a procedure previously used by Pham et al (1991). The peptide (18 µg (0-2 M, pH 7-5), was added to a vial containing 2 mCi, 74 MBq Na125I in 20 µ NaOH solution.…”

Section: Subjectsmentioning

confidence: 99%

Plasma levels and molecular forms of proatrial natriuretic peptides in healthy subjects and in patients with congestive heart failure

Azizi

Maistre²,

Kalotka³

et al. 1996

Journal of Endocrinology

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A specific and sensitive radioimmunoassay (RIA) for the N-terminal fragment of proatrial natriuretic peptide (NproANP) was developed. Antiserum raised in rabbits against a mixture enriched with prohormone was 100% cross-reactive with human proANP(1-30). Plasma concentrations of proANP(1-30) and ANP immunoreactivities (ir-) were simultaneously measured in healthy subjects and patients with congestive heart failure (CHF; 26 dilated cardiomyopathy and 5 ischemic heart disease). High plasma levels of both ir-proANP(1-30) and ir-ANP were detected in CHF patients. Circulating ir-ANP levels were elevated in New York Heart Association functional Classes II and III patients but not in Class I patients. However, plasma levels of ir-proANP(1-30) were higher in asymptomatic patients than in healthy subjects, and markedly increased in patients of Classes II and III. Analysis of ir-proANP(1-30) by gel filtration chromatography or reverse-phase high pressure liquid chromatography revealed a 10 kDa peptide circulating as a distinct entity. These findings indicate that: (i) the most probable form of NproANP in human plasma is a 10 kDa peptide and (ii) in CHF patients the rise in plasma ir-proANP(1-30) levels is more pronounced than the variation in plasma ir-ANP. Thus, NproANP plasma levels may prove to be a more sensitive marker of left ventricular dysfunction than ANP.

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Section: Subjectsmentioning

confidence: 99%

Plasma levels and molecular forms of proatrial natriuretic peptides in healthy subjects and in patients with congestive heart failure

Azizi

Maistre²,

Kalotka³

et al. 1996

Journal of Endocrinology

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“…2 In order to achieve the peptide labelling, there are a number of other radioisotopes that can be used, such as tritium and iodine-125. 3 Labelling with iodine-125 normally involves incorporation of iodine-125 into tyrosine or histidine residues within the peptide or protein. 4 However, the benefit of using 14 C for ADME programmes is that the label is placed within the core of the drug.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of carbon‐14–labelled peptides

Dell'Isola

Brown

Jones

et al. 2019

Labelled Comp Radiopharmac

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Carbon‐14 (14C)–labelled active pharmaceutical ingredients (APIs) and investigational medicinal products (IMPs) are required for phase 0/I to phase III mass balance and micro‐dosing clinical trials. In some cases, this may involve the synthesis of 14C‐labelled peptides, and the analysis can be performed by accelerated mass spectrometry (AMS). The 14C‐peptide is typically prepared by the solid‐phase peptide synthesis (SPPS) approach using custom‐made glassware for the key coupling steps. Further modification of the purified 14C‐peptide can then be performed.

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Radiohalogen incorporation into organic systems

Bolton

2002

Labelled Comp Radiopharmac

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SummaryThis Review reports the more recent applications of radiohalogen labelling in organic compounds. Modern synthetic methods place more emphasis upon substitution at specific sites. Electrophilic substitution, especially at aromatic carbon atoms, involves displacement of halogen or of metals rather than the earlier popular halogen-deprotiation processes. Nucleophilic displacement, at either aliphatic or aromatic sites, has also become widespread since it may be made more selective by the inclusion of appropriate activating groups or suitable displaced groups. Microwave and thermally induced methods are both reported with a critical assessment of the value of each process.

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Radiolabelling by tritium and [¹²⁵I]iodine of an angiotensin II related Peptide

Abstract: The labelling of this peptide with 1251 was performed by two ways :

Cited by 3 publications

References 6 publications

Plasma levels and molecular forms of proatrial natriuretic peptides in healthy subjects and in patients with congestive heart failure

Plasma levels and molecular forms of proatrial natriuretic peptides in healthy subjects and in patients with congestive heart failure

Synthesis of carbon‐14–labelled peptides

Radiohalogen incorporation into organic systems

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Radiolabelling by tritium and [125I]iodine of an angiotensin II related Peptide

Abstract: The labelling of this peptide with 1251 was performed by two ways :

Cited by 3 publications

References 6 publications

Plasma levels and molecular forms of proatrial natriuretic peptides in healthy subjects and in patients with congestive heart failure

Plasma levels and molecular forms of proatrial natriuretic peptides in healthy subjects and in patients with congestive heart failure

Synthesis of carbon‐14–labelled peptides

Radiohalogen incorporation into organic systems

Contact Info

Product

Resources

About

Radiolabelling by tritium and [¹²⁵I]iodine of an angiotensin II related Peptide