Radioiodination of recombinant human growth hormone and its use in radioimmunoassay

Abstract

Radioimmunoassay (RIA) of human growth hormone (hGH) using lz51-1abeled tracer prepared from DNA recombinant hGH (r-hGH) and characterization of the tracer in the assay system are described. The radioiodination of r-hGH resulted in high yield of immunoreactive tracer. The immunoreactive fraction could be purified by geI-filtration on sephadex G-75. The quality of radioiodinated tracer of r-hGH has been found to be same as that of the tracer obtained from pituitary hGH (p-hGH) with respect to immunoreactivity, assay sensitivity and RIA standard curve parameters.

A chemiluminescent metalloimmunoassay based on copper‐enhanced gold nanoparticles for quantification of human growth hormone

“…5. The response characteristic of the proposed method was compared with recently reported methods for the determination of hGH (18)(19)(20)(21)(22) and the results are given in Table 1. A linear range was obtained from 0.2 to 50 ng/mL using the linear equation y = À1.06 log x + 3.04 (r 2 = 0.968), where y is the CL intensity and x is the concentration of hGH in ng/mL (Fig.…”

“…The relative standard deviation (RSD) for four measurements of 10 ng/mL hGH was 6.2%. The response characteristic of the proposed method was compared with recently reported methods for the determination of hGH (18)(19)(20)(21)(22) and the results are given in Table 1. It can be seen that the proposed method has a wider dynamic range than other reported hGH immunosensors.…”