Radioimmunoassay to Detect Antitransglutaminase Autoantibodies Is the Most Sensitive and Specific Screening Method for Celiac Disease

Bonamico, Margherita; Tiberti, Claudio; Picarelli, Antonio; Mariani, P; Rossi, Dario; Cipolletta, E; Greco, Marco; Tola, Marco Di; Sabbatella, L.; Carabba, Barbara; Magliocca, Fabio Massimo; Strisciuglio, Pietro; Mario, U. Di

doi:10.1111/j.1572-0241.2001.03754.x

Cited by 87 publications

(56 citation statements)

References 36 publications

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“…This was probably a result of the presence of conformational domains in the tTG protein 37,38 that seem to be better preserved in fluidphase rather than in solid-phase procedures. 27 Previously, several attempts were made to detect salivary CD-specific antibodies, in particular AGA and EMA. 28-34 AGA detection had generally low sensitivity, and when used to screen children with CD, also a limited specificity.…”

Section: Discussionmentioning

confidence: 99%

“…tTG-Ab presence was detected by a previously published RIA method, where a receiver operator characteristic (ROC) analysis was used to identify the optimal threshold value for sensitivity and specificity. 27 Ab levels were expressed as an Ab index calculated as follows: (sample counts per minute [cpm] -negative standard sample cpm)/(positive standard control cpm -negative standard control cpm). Serum samples were considered tTG-Ab-positive if the Ab index was above 0.050.…”

Section: Ema Methodsmentioning

confidence: 99%

“…27 Briefly, [ 35 S]-methionine tTG was incubated at 48C with 30 lL of saliva sample diluted in buffer solution. Twenty-five microliters of goat anti-human IgA-Agarose (Sigma, St. Louis, Mo) were added subsequently to separate free from Ab-bound labeled products.…”

Section: Saliva Ttg-ab Riamentioning

confidence: 99%

“…Using previously ROC-plot-established thresholds, corresponding to the 99th percentile of a control population, 27 39 of 39 (100%) Group 1 patients with CD had serum tTGAbs (Fig 2, left side) with a mean Ab index of 0.783 ± 0.356; 35 of 39 (89.7%) Group 1 patients with CD had high tTG-Ab titers. tTG-Abs were not detected in Group 2 and Group 3 subject sera.…”

Section: Ttgabs In Serummentioning

confidence: 99%

“…23,24 Several methods have been proposed for the detection of humoral anti-tTG immunoreactivity in CD. 20,[25][26][27] The most sensitive assays to detect tTG-Abs were those using human recombinant tTG in a fluid-phase radioimmunoassay (RIA) format. 26,27 Attempts have been made in assaying CD-related Ab in saliva, [28][29][30][31][32][33][34][35] which are easily obtained by noninvasive techniques, bypassing blood sample collection.…”

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confidence: 99%

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Tissue transglutaminase autoantibody detection in human saliva: a powerful method for celiac disease screening

Bonamico

Ferri

Nenna

et al. 2004

The Journal of Pediatrics

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Objective To test the possibility of detecting tissue transglutaminase autoantibodies (tTG-Abs) in saliva with a novel sensitive fluid-phase radioimmunoassay (RIA).Study design Paired saliva and serum samples from 39 patients with celiac disease (CD), at the first biopsy (Group 1: 28 females, mean age 11.5 ± 11.1 years); 32 controls with a normal duodenal mucosa (Group 2: 18 females, mean age 8.1 ± 3.6 years); and 32 healthy volunteers (Group 3: 21 females, mean age 31.7 ± 9.8 years) were studied for tTG-Ab presence. Limit of positivity for salivary assay was calculated according to the 99th percentiles of Group 2 control children and was expressed as an autoantibody (Ab) index.Results Salivary tTG-Abs were found in 97.4% of the patients with CD and in 100% of the corresponding serum samples. All Group 3 subjects were negative with both saliva and serum assays. A correlation between saliva and serum tTG-Ab titers was found (r = 0.826, P = .0014).Conclusions This study demonstrates that it is possible to detect salivary tTG-Abs in CD with a non-invasive, simple to perform, reproducible and sensitive method. (J Pediatr 2004;144:632-6) C eliac disease (CD) may appear in a classic presentation, with gastrointestinal complaints and growth failure or with extraintestinal manifestations and ''atypical forms'' 1 characterized by anemia and short stature, 2,3 or with a silent form, more frequent in first-degree relatives of patients with CD. 4 The prevalence of CD is increased in subjects with elevated aminotransferase levels, 5,6 autoimmune diseases, 7,8 and chromosomal aberrations. 9,10 Complications of long-standing CD may include osteopathy, 11 endocrinopathy, 12 infertility, low birth weight infants, 13,14 cancer, 15 and dilated cardiomyopathy and other forms of heart failure. 16 CD has a high prevalence in the general population (0.55% in Italy), often in an asymptomatic form. 17 To reduce the risks associated with prolonged gluten exposure, CD should be diagnosed early, on the basis of the result of one or more intestinal biopsies. 18 Patients with CD receiving a gluten-containing diet have increased levels of anti-gliadin (AGA), anti-endomysium (EMA), 19 and anti-tissue transglutaminase autoantibodies (anti-tTG-Ab). 20 Serum AGA have proved to be effective in the identification of children at risk for CD, but less so in adult screening. 21 EMA are more specific than AGA but are observer-dependent, and sometimes they are not found in children with CD younger than aged 2 years. 19 The recent identification of tTG as the main autoantigen recognized by EMA 22 has lead to the development of various studies that suggest an important role for this enzyme in the etiopathogenesis of CD. 23,24 Several methods have been proposed for the detection of humoral anti-tTG immunoreactivity in CD. 20,[25][26][27] The most sensitive assays to detect tTG-Abs were those using human recombinant tTG in a fluid-phase radioimmunoassay (RIA) format. 26,27 Attempts have been made in assaying CD-related Ab in saliva, [28][29][30][31][32][...

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Section: Discussionmentioning

confidence: 99%

Section: Ema Methodsmentioning

confidence: 99%

Section: Saliva Ttg-ab Riamentioning

confidence: 99%

Section: Ttgabs In Serummentioning

confidence: 99%

mentioning

confidence: 99%

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Tissue transglutaminase autoantibody detection in human saliva: a powerful method for celiac disease screening

Bonamico

Ferri

Nenna

et al. 2004

The Journal of Pediatrics

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Diagnosing Celiac Disease

Baudon¹,

Johanet²,

Absalon³

et al. 2004

Arch Pediatr Adolesc Med

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Risk of Celiac Disease Autoimmunity and Timing of Gluten Introduction in the Diet of Infants at Increased Risk of Disease

Norris

2005

JAMA

385

336

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ELIAC DISEASE, ALSO CALLED gluten-sensitive enteropathy, is characterized by chronic inflammation in the small intestine, resulting in villous atrophy and flattening of the mucosa, induced by prolamins (gluten) present in wheat, barley, or rye. 1,2 The classic form of celiac disease typically presents in early childhood with abdominal pain and diarrhea, malabsorption, and nutrient deficiencies. Most patients with celiac disease carry the human leukocyte antigen HLA-DRB1*03 allele (usually associated with HLA-DQ2) or HLA-DRB1*04 (associated with HLA-DQ8). 1,3 These alleles also confer increased risk for type 1 diabetes; thus, individuals with type 1 diabetes and their first-degree relatives have increased risk of celiac disease. 4 However, few genetically susceptible individuals develop ce-See also p 2410 and Patient Page.

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Radioimmunoassay to Detect Antitransglutaminase Autoantibodies Is the Most Sensitive and Specific Screening Method for Celiac Disease

Cited by 87 publications

References 36 publications

Tissue transglutaminase autoantibody detection in human saliva: a powerful method for celiac disease screening

Tissue transglutaminase autoantibody detection in human saliva: a powerful method for celiac disease screening

Diagnosing Celiac Disease

Risk of Celiac Disease Autoimmunity and Timing of Gluten Introduction in the Diet of Infants at Increased Risk of Disease

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