Radioimmunoassay of β-MSH in Human Plasma and Tissues*

Abe, Kaoru; Nicholson, Wendell E.; Liddle, Grant W.; Island, Donald P.; Orth, David N.

doi:10.1172/jci105653

Cited by 100 publications

(24 citation statements)

References 14 publications

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“…The secretion of several anterior pituitary hormones in man has been shown previously to be altered by glucocorticoids. That these agents regulate ACTH secretion is well known and effects on MISH secretion have also been described (17). Impairment of the increase in plasma growth hormone (GH) which follows insulin-induced hypoglycemia during glucocorticoid administration has been reported during some but not all experimental conditions (18)(19)(20)(21), whereas the rise in plasma GH provoked by arginine infusion is not impaired by short-term steroid treatment (20).…”

Section: Discussionmentioning

confidence: 99%

The effect of glucocorticoids on thyrotropin secretion

Wilber¹,

Utiger²

1969

J. Clin. Invest.

271

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A B S T R A C T The effect of large doses of glucocorticoids on thyrotropin (TSH) secretion in normal and hypothyroid humans has been studied. Plasma TSH concentrations were measured before, during, and after treatment with dexamethasone given orally for 24-48 hr. In 17 patients with primary hypothyroidism, plasma TSH levels fell significantly during treatment to a mean of 54% of control (range 23-96%). Within 48 hr after the withdrawal of dexamethasone, TSH concentrations transiently increased above pretreatment values. The mean increase was to 156% of control (range . Similar changes, but of smaller magnitude, were observed in 15 normal subjects. Administration of single oral doses of dexamethasone and oral or intravenous doses of cortisol were followed by reduction of plasma TSH levels to 18-47% of control within 8-12 hr in eight hypothyroid patients. This fall also was followed by significant TSH rises above control values before they returned to the pretreatment levels. Mineralocorticoid administration was not followed by any changes in plasma TSH concentrations in three subjects.TSH responses to steroid were also studied in rats. In hypothyroid rats given dexamethasone intravenously, plasma TSH fell to 63% of control in 30-90 min and then returned to normal or above in 3-4 hr. Dexamethasone also reduced plasma TSH concentrations in normal rats but no rebound was observed in these animals. Dexamethasone did not block the increase in plasma TSH produced by thyrotropin releasing factor (TRF) administration in vivo. Neither basal nor TRF-mediated TSH release from hemipituitaries in vitro was reduced by dexamethasone or corticosterone. These studies indicate that glucocorticoids reduce TSH secretion and suggest that this effect occurs at a suprahypophyseal level.

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Section: Discussionmentioning

confidence: 99%

The effect of glucocorticoids on thyrotropin secretion

Wilber¹,

Utiger²

1969

J. Clin. Invest.

271

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“…8-MSH was extracted from plasma or tissue and subjected to radioimmunoassay by a method described previously (1 Metyrapone and dexamethasone tests. 13 normal subjects received 0.75 g of metyrapone orally every 4 hr for 1 day.…”

Section: Methodismentioning

confidence: 99%

“…,8-Melanocyte-stimulating hormone (8-MSH) has previously been identified and quantified in the plasma of normal subjects and patients with addisonian hyperpigmenReceived for publication 25 February 1969. tation by means of radioimmunoassay (1). On the basis of these studies it was concluded that fi-MSH is probably the main pigmentary hormone in man and that the degree of addisonian hyperpigmentation correlates well with the degree of elevation of plasma fi-MSH.…”

Section: Introductionmentioning

confidence: 98%

Normal and abnormal regulation of β-MSH in man

Abe¹,

Nicholson²,

Liddle³

et al. 1969

J. Clin. Invest.

Self Cite

122

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“…Intracytoplasmic granules in our two cases resembled normal human pancreatic zymogen granules in structure and size (630 nm in mean diameter) and were somewhat smaller in size than normal human salivary zymogen granules (1,200 ules were condensing and storing sites of digestive enzymes. In normal rat and bovine pancreas, the number of zymogen granules per cell was reported to be well correlated with tissue amylase content after the stimulation by pilocarpine or other cholinergic drug.…”

Section: Discussionmentioning

confidence: 48%

Ultrastructural, histochemical, and biochemical studies of two cases with amylase, ACTH, and β-MSH producing tumor

Gomi

Kameya²,

Tsumuraya³

et al. 1976

Cancer

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Two cases of intrathoracic tumor, different in histology and accompanied by hyperamylasemia, were studied ultrastructurally, histochemically, and biochemically. The ultrastructure of the tumor cell cytqplasm showed many zymogen granules in case 1 and smaller cored granules in addition to zymogen granules in case 2 . Both tpmors contained not only a large amount of amylase, which was electrophoretically of saliva type with three components, but also significant amounts of immunoreactive ACTH and @-MSH. Starch film and immunofluorescence showed that the tumor cells stored amylase. It was concluded from these findings that the tumor cells ectopically producing amylase, which showed differentiation toward the cells with zymogen production, could differentiate toward the cells of ACTH-MSH system at the same time.

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Radioimmunoassay of β-MSH in Human Plasma and Tissues*

Cited by 100 publications

References 14 publications

The effect of glucocorticoids on thyrotropin secretion

The effect of glucocorticoids on thyrotropin secretion

Normal and abnormal regulation of β-MSH in man

Ultrastructural, histochemical, and biochemical studies of two cases with amylase, ACTH, and β-MSH producing tumor

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