Radioimmunoassay of Arginine Vasopressin in Human Plasma

Beardwell, Colin G

doi:10.1210/jcem-33-2-254

Cited by 123 publications

(69 citation statements)

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“…These changes in plasma AVP were found to correlate significantly with changes in plasma osmolalitv (Fig. 7), a relationship also observed w-ith our previous technique (1) and more recently by others using bioassay (24) as well as immunoassay (12). As indicated by the equation describing this relationship, the osmoreceptor governing AVP release had a threshold around 280 mosmol/kg and, in agreement with the original conclusions of Verney (25), was sensitive to increases in plasma osmolality as small as 1%.…”

Section: Resultssupporting

confidence: 84%

“…DISCUSSION The development of a clinically useful radioimmunoassay for plasma vasopressin has proven to be more difficult than for many of the other polypeptide hormones. Although a number of other laboratories have succeeded by a variety of methods in raising antisera to vasopressin (7)(8)(9)(10)(11)(12)(13)(14)(15), none has been sufficiently sensitive to detect the hormone at the low concentrations (10-M) normally present in plasma. Moreover, when carefully studied, several of these antisera have been found to be unusually susceptible to interference by a The effect of acute hypotension on plasma AVP in a healthy subject.…”

Section: Resultsmentioning

confidence: 99%

“…-* * variety of plasma components (1,12). Because of these * problems it previously has been necessary to extract chromatographically and concentrate plasma vasopressin before assay, a requirement that significantly limits the usefulness of the technique.…”

Section: Resultsmentioning

confidence: 99%

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Development and Clinical Application of a New Method for the Radioimmunoassay of Arginine Vasopressin in Human Plasma

Robertson¹,

Mahr²,

Athar³

et al. 1973

J. Clin. Invest.

774

299

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Section: Resultssupporting

confidence: 84%

Section: Resultsmentioning

confidence: 99%

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Development and Clinical Application of a New Method for the Radioimmunoassay of Arginine Vasopressin in Human Plasma

Robertson¹,

Mahr²,

Athar³

et al. 1973

J. Clin. Invest.

774

299

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“…This syndrome is due to inappropriate hyperfunction of antidiuretic hormone-like substances (ADH) and is termed inappropriate ADH secretion [2][3]. Subsequent studies demonstrated that in most patients with SIAD, plasma levels of arginine vasopressin (AVP) are inappropriately high [4][5][6]. Further, Robertson et al reported that various types of osmotic AVP secretion are present in SIAD [7][8][9].…”

mentioning

confidence: 99%

Osmoregulation of Vasopressin Secretion in Patients with the Syndrome of Inappropriate Antidiuresis Associated with Central Nervous System Disorders.

et al. 1999

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Abstract.To clarify the characteristics of vasopressin (AVP) secretion in patients with the syndrome of inappropriate antidiuresis (SIAD) related to central nervous system disorders, we examined the response of AVP secretion to osmotic stimulus by hypertonic saline infusion and analyzed the possible causative factors in six patients with SIAD associated with head trauma or cerebral infarction. Hyponatremia developed after head trauma in four patients and cerebral infarction in two patients.In all patients the clinical state and laboratory findings fulfilled the criteria for SIAD, which was supported by either nonsuppressible plasma AVP levels or effectiveness of treatments with water restriction, demeclocycline, nonpeptide V2 AVP antagonist or diphenylhydantoin.Although patterns of plasma AVP response to the osmotic stimulus varied, plasma AVP concentrations neither increased nor decreased to undetectable levels with a rise in plasma osmolality. In one patient, plasma AVP levels responded to increasing plasma osmolality when plasma osmolality normalized; in which the threshold and the sensitivity of osmostat were normal. In two other patients, AVP secretion responded to plasma osmolality after the treatment.The changes in AVP secretion were not due to nonosmotic stimuli for AVP release.In conclusion, this study shows that patients with SIAD and central nervous system disorders may have persistent AVP secretion with a loss of hypotonic suppression such as found in patients with adrenal insufficiency or depletional hyponatremia in central nervous system disorders, indicating that careful evaluation is necessary to determine the relationship between persistent AVP secretion and the pathogenesis of hyponatremic disorders.

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“…The labeled ADH used in these reports was of low specific activity, which required administration of supraphysiological quantities of hormone. Only limited data on ADH metabolism from radioimmunoassays have been reported to date (5)(6)(7)(8). In the present report we have used radioiodinated arginine vasopressin (AVP) to study the metabolism of ADH in man at physiological plasma concentrations.…”

Section: Introductionmentioning

confidence: 99%

Distribution, blood transport, and degradation of antidiuretic hormone in man.

Baumann¹,

Dingman²

1976

J. Clin. Invest.

159

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A BS TR A CT The distribution, blood transport, and metabolic clearance of physiological concentrations of antidiuretic hormone were studied in 10 hydrated normal subjects with radioiodinated arginine vasopressin ('25I-AVP). At 370C no binding of '"I-AVP to plasma proteins could be demonstrated, but some metabolites were associated with plasma proteins. "I-AVP was rapidly distributed into a space approximating the extracellular fluid volume. Metabolic breakdown products became demonstrable within minutes after injection. The mean metabolic clearance rate of 'I-AVP was 4.1 ml/min/kg and the mean plasma half-life 24.1 min. Renal clearance had a mean value of 80 ml/min and accounted for 27% of the total metabolic clearance. It is concluded that in man antidiuretic hormone circulates as a free (non-protein bound) peptide, diffuses readily into the extracellular fluid space, and is metabolized within minutes. A plasma half-life of 24 min is consistent with the duration of antidiuresis after hormone administration or release.

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Radioimmunoassay of Arginine Vasopressin in Human Plasma

Cited by 123 publications

References 0 publications

Development and Clinical Application of a New Method for the Radioimmunoassay of Arginine Vasopressin in Human Plasma

Development and Clinical Application of a New Method for the Radioimmunoassay of Arginine Vasopressin in Human Plasma

Osmoregulation of Vasopressin Secretion in Patients with the Syndrome of Inappropriate Antidiuresis Associated with Central Nervous System Disorders.

Distribution, blood transport, and degradation of antidiuretic hormone in man.

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