Radioimmunoassay of alpha rat atrial natriuretic peptide

Anderson, J. V.; Christofides, Nicos; Viñas, Paula; Wharton, John; Varndell, Ian M.; Polak, J.M.; Bloom, S.R.

doi:10.1016/0143-4179(86)90092-2

Cited by 29 publications

(11 citation statements)

References 23 publications

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“…In addition, the cross-reactivity of the antiserum with the complete pro-ANP molecule (although this could not be determined precisely because synthetic pro-ANP was not available) was demonstrated by the parallelism obtained between serial dilutions of human and of rat right atrial extracts and the standard curve of synthetic CDN 1-16. This specific cross-reactivity is further substantiated by the highly significant correlation found in human right atrial extracts between cardiodilatin-IR concentrations, measured by the antiserum to the N-terminal of pro-ANP, and ANP-IR concentrations, measured by an established radioimmunoassay [21] with an antiserum to the C-terminal of pro-ANP. Therefore, this newly developed radioimmunoassay offers an alternative to the ANP radioimmunoassay for the estimation of pro-ANP concentrations in human atrial tissues.…”

Section: Discussionmentioning

confidence: 78%

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The presence and molecular forms of cardiodilatin immunoreactivity in the human and rat right atrium

Meleagros

Ghatei

Anderson

et al. 1988

Clinica Chimica Acta

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A sensitive and specific radioimmunoassay has been developed for cardiodilatin; the N-terrninal peptide sequence of the atrial natriuretic peptide (ANP) prohorrnone. Cardiodilatin-immunoreactivity (-IR) concentrations in the human right atrial appendage we re found to correlate with ANP-IR concentrations, deterrnined by an established radioimmunoassay, (cardiodilatin-IR = 13.2 ± 1.2 nmoljg, ANP-IR = 19.8 ± 2.0 nmoljg, r = 0.80, p < 0.001). Characterisation of the cardiodilatin-IR in the human and rat right atrium by gel permeation and fast protein liquid chromatography revealed only two cardiodilatin-IR molecular forms. The larger more hydrophobic form, the majority of the cardiodÜatin-IR, contained in addition ANP-IR and therefore represents the prohorrnone. The smaller, less hydrophobic form, lacked ANP-IR and thus represents the cleaved N-terrninal peptide sequence of the prohorrnone. These findings indicate that the prohorrnone is the major molecular form in the human and rat atrium. Furthermore, they demonstrl:lte that a single large N-terrninal peptide, cardiodilatin, derived from the prohorrnone, may exist as a distinct molecular form in the atrium of these species.

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Section: Discussionmentioning

confidence: 78%

“…ANP-immunoreactivity was measured by a weil established radioimmunoassay developed in our laboratory [21].…”

Section: Assay Conditionsmentioning

confidence: 99%

The presence and molecular forms of cardiodilatin immunoreactivity in the human and rat right atrium

Meleagros

Ghatei

Anderson

et al. 1988

Clinica Chimica Acta

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“…Immunostaining of 4-gm-thick tissue sections was done using the peroxidase-antiperoxidase method of Sternberger (27) and counterstained with hematoxylin. Primary rabbit antisera were raised against synthetic a human ANP (Peninsula Laboratories, San Carlos, CA), as previously described (19), and used at dilutions of 1:800-1600.…”

Section: Methodsmentioning

confidence: 99%

“…However, in nonmammalian species ANP-like immunoreactivity occurs in the ventricles as well as the atria (18), and the results of mRNA blot analysis (6,16) and radioimmunoassay (15,19) together indicate at least some expression of the ANP gene in the ventricles of the rat, although normally at a lower level than in the atria. While the presence of atrial natriuretic peptides in ventricular cells might result from their synthesis at these sites, the possible uptake of circulating ANP cannot be excluded (15).…”

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confidence: 99%

“…The nucleotide sequence of the genomic DNA encoding prepro-ANP has also been determined (6)(7)(8)(9)(10)(11) and in man the gene is known to consist of 2 kilobase pairs, comprising three exons and two introns, located on the distal short arm of chromosome 1 (12). The ANP gene is expressed mainly in atrial tissues that contain both ANP transcripts (6,(13)(14)(15)(16) and ANP immunoreactivity, the latter being localized to secretory granules in the atrial myocytes (17)(18)(19).…”

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confidence: 99%

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Localization of atrial natriuretic peptide mRNA and immunoreactivity in the rat heart and human atrial appendage.

Hamid

Wharton

Terenghi

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

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131

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The localization of mRNA encoding preproatrial natriuretic peptide was investigated in tissue sections and cultures of rat heart and in sections of human right atrial appendage using the technique of in situ hybridization with 32P-and 35S-labeled RNA probes. Rat atrial natriuretic peptide (ANP) transcripts were demonstrated in numerous atrial myocytes and, to a lesser extent, in ventricular myocytes in both tissue sections and newborn rat heart cultures. These rmdings are consistent with those obtained by RNA blot analysis of rat heart total RNA, indicating that a single prepro-ANP transcript of ""900 nucleotides was present in the ventricles as well as the atria. Using a 35S-labeled RNA probe for human ANP mRNA, ANP transcripts were also localized to the majority of myocytes in the human right atrial appendage. Only background levels of autoradiographic labeling were obtained when RNA probes identical to the coding sequence of rat or human ANP mRNA were used. A close correlation was found between the distribution of ANP immunoreactivity and prepro-ANP mRNA in these preparations. These results provide unequivocal evidence for the expression of the ANP gene in the rat ventricles, as well as the atria, because myocytes in these tissues have been established as the sites of both ANP localization and precursor biosynthesis. The combined use of cardiac cultures and in situ hybridization may be of value in future studies investigating the regulation of ANP synthesis in cardiac myocytes.Cloning and sequence analysis of the complementary DNA (cDNA) encoding rat and human atrial natriuretic peptide (ANP) mRNA has revealed the structure of the prepro-ANP molecule, which consists of 151 and 152 amino acids in the human and rat, respectively (1-5). The nucleotide sequence of the genomic DNA encoding prepro-ANP has also been determined (6-11) and in man the gene is known to consist of 2 kilobase pairs, comprising three exons and two introns, located on the distal short arm of chromosome 1 (12). The ANP gene is expressed mainly in atrial tissues that contain both ANP transcripts (6, 13-16) and ANP immunoreactivity, the latter being localized to secretory granules in the atrial myocytes (17-19).However, in nonmammalian species ANP-like immunoreactivity occurs in the ventricles as well as the atria (18), and the results of mRNA blot analysis (6, 16) and radioimmunoassay (15, 19) together indicate at least some expression of the ANP gene in the ventricles of the rat, although normally at a lower level than in the atria. While the presence of atrial natriuretic peptides in ventricular cells might result from their synthesis at these sites, the possible uptake of circulating ANP cannot be excluded (15). Furthermore, immunocytochemical techniques alone are not very useful for examining the regulation of prepro-ANP production because it is not possible to distinguish between changes in synthesis and secretion. In situ hybridization provides a method for demonstrating gene expression at the cellular level and may be of value ...

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