Radioimmunoassay Measurement of Arginine Vasopressin in Serum: Development and Application

Skowsky, W. Ronald; Rosenbloom, A.A.; Fisher, Delbert A.

doi:10.1210/jcem-38-2-278

Cited by 336 publications

(139 citation statements)

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“…Regression coefficients of AVP change over time also differed significantly between groups (p <0.05) with a linear increase characterizing the TM group. Mean pAVP values for the rest group were within the normal range for measurements m this laboratory on healthy subjects (0,75_+0.50 ~zU/ml; [31]). pAVP levels in the single, separate samples drawn at a different Ume of day from those of the time series experiment were also w~thin the normal range in meditation subjects.…”

Section: Methodssupporting

confidence: 53%

“…Mean pAVP values for the rest group were within the normal range for measurements m this laboratory on healthy subjects (0,75_+0.50 ~zU/ml; [31]). pAVP levels in the single, separate samples drawn at a different Ume of day from those of the time series experiment were also w~thin the normal range in meditation subjects.Plasma osmolality values for both groups were within the range of values for normal ambulatory subjects (289_+5: [24,31]). Plasma osmolality did not vary s~gnificantly over time for either group, nor was there any significant difference between groups at any time point.…”

supporting

confidence: 53%

“…Plasma osmolality values for both groups were within the range of values for normal ambulatory subjects (289_+5: [24,31]). Plasma osmolality did not vary s~gnificantly over time for either group, nor was there any significant difference between groups at any time point.…”

Section: Methodsmentioning

confidence: 63%

“…Plasma arginme vasopressin (pAVP) was quantitated under doubleblind conditions using a highly specific radimmmunoassay [31]. Radioimmunoassay characterlstms Include a sensmvlty of 0 2 jzU/ml (0.6 pg/ml) and a C,V of 4.8% within assay and 9.6% between assay.…”

Section: Methodsmentioning

confidence: 99%

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Hormonal control in a state of decreased activation: Potentiation of arginine vasopressin secretion

O’Halloran

Jevning

Wilson

et al. 1985

Physiology & Behavior

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PHYSIOL BEHAV 35(4) 591-595, 1985 --Behaviorally induced stress is associated with increased arginme vasopressln (AVP) secretion. In this report we describe a phasic conditioned response of AVP secretion yielding 2.6--7.1 times normal plasma concentration of this hormone m assocmtlon w~th a physmlog~cal state of decreased activation, that associated with the mental technique of "transcendental meditation" (TM) in long-term practltmners (6-8 years of regular elicitation) Such a very large phastc response of AVP was previously unknown m the normal physmlogy of AVP. This elevation was not accompanied by elevatmn of plasma osmolality. Unstyllzed ordinary eyes closed rest in a separate group of subjects studied in the same manner was associated with normal plasma AVP concentration. Galvanic skin resistance (GSR) increased dunng both TM and rest with significantly larger increase assocmted with TM. Other measures of activation, including muscle metabolism, and the Spielberger Anxiety Inventory indicated marked relaxatmn in associatmn with TM. In previous research it has been shown that blood pressure does not change acutely dunng th~s behavmr. These observations in&cate that neither stress nor operatmn of other usual homeostatic control mechamsms are responsible for elevated for AVP in the meditators. It is speculated that the apparently unique mechanism of TM-mduced AVP secretion may be more specifically related to the behavmral effects of meditatmn. [5,7,8] and, more specifially, the acquisition and retention of adaptive behavior patterns [4,8]. Additional reported effects of AVP in humans suggest psychotherapeutic and behavior-modifying properties [9,34] as well as improvement of attentional processes [ 1]. Implicit in many of these reports is the notion that naturally occurring modification of existing, or acquisition of new behavior patterns may be facilitated by an increase of endogenous AVP activity centrally and/or peripherally. However, except for states of stress, potentiation of AVP secretion associated with behavior modification or adaptation has not been demonstrated. The purpose of the present study was to determine if long-term, regular practice of the technique of "transcendental meditation" (TM), reported to induce numerous behavioral changes [10,12,16,22,28], may be associated with altered secretion of AVP. This behavior, elicited twice daily in the morning and evening for 20--40 minute periods by the regular practitioner, is associated with major physiologic changes in long-term subjects [17][18][19]. Hormones METHODTwelve subjects, six long-term meditation practitioners (5-10 year instructors of TM) and six non-meditating individuals ("ordinary rest" group), were studied. All subjects were normal, university educated individuals and did not use tobacco, alcohol or regular medication. Their ages ranged 1Requests for reprints should be addressed to R. Jevning, Departments of Medicine and Physiology, University of Califorma, Irvine, Irvme, CA 92717. The additional point for the TM group represent...

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Section: Methodssupporting

confidence: 53%

supporting

confidence: 53%

Section: Methodsmentioning

confidence: 63%

Section: Methodsmentioning

confidence: 99%

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Hormonal control in a state of decreased activation: Potentiation of arginine vasopressin secretion

O’Halloran

Jevning

Wilson

et al. 1985

Physiology & Behavior

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“…For vasopressin the radioimmunoassay used after bentonite extraction (Skowsky et al, 1974) had a sensitivity of 0.3pgml-P. The recovery of vasopressin was 75% and intra and interassay coefficients of variation were 3 and 11%, respectively.…”

Section: Measurement Ofplasma Hormone Levelsmentioning

confidence: 99%

Cardiovascular effects of central injection of acetylcholine in anaesthetized dogs: a role for vasopressin release

Rascol

Montastruc

Gauquelin³

et al. 1990

British J Pharmacology

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1 The effect of an intracisternal injection of 20Ougkg-' of acetylcholine was studied on systolic and diastolic blood pressures, heart rate, and plasma levels of noradrenaline, adrenaline, vasopressin, plasma renin activity and atrial natriuretic factor in chloralose-anaesthetized dogs, 8 of which were normal and 7 with diabetes insipidus (deprived of vasopressin secretion by surgical lesion of the hypothalamoneurohypophysial system). 2 Acetylcholine significantly increased systolic and diastolic blood pressures in both groups of animals. However, the rise in blood pressure was significantly shorter lived in the dogs with diabetes insipidus. 3 Acetylcholine significantly increased plasma levels of noradrenaline but not adrenaline in control animals and in dogs with diabetes insipidus. Noradrenaline and adrenaline responses after acetylcholine were not different in the two groups of animals. 4 Acetylcholine induced a significant increase in vasopressin plasma levels only in control animals while in dogs with diabetes insipidus vasopressin remained at nearly undetectable levels. 5Acetylcholine significantly increased atrial natiuretic factor plasma levels only in control dogs. 6 Although plasma renin activity increased in both groups of animals after the i.c. injection of acetylcholine, this change was not significant in any group. 7 These results suggest that, in the anaesthetized dog, the central injection of acetylcholine induces a rise in blood pressure through both an increase in sympathetic outflow and a release of vasopressin.

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Hyponatremia of Hypothyroidism

Macaron¹,

Famuyiwa²

1978

Arch Intern Med

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Radioimmunoassay Measurement of Arginine Vasopressin in Serum: Development and Application

Cited by 336 publications

References 0 publications

Hormonal control in a state of decreased activation: Potentiation of arginine vasopressin secretion

Hormonal control in a state of decreased activation: Potentiation of arginine vasopressin secretion

Cardiovascular effects of central injection of acetylcholine in anaesthetized dogs: a role for vasopressin release

Hyponatremia of Hypothyroidism

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