Radioimmunoassay, complement fixation and counter-immunoelectrophoresis in the laboratory diagnosis of hepatitis B

Kouroupis, G. M.; Leers, W. D.

doi:10.1007/bf01249354

Cited by 6 publications

(6 citation statements)

References 10 publications

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“…However, it lacks a certain degree of specificity by showing false positive results. This fault could be corrected satisfactorily by absorbing the sera with anti-HB-Ab and retesting (7). Modification of the Ausria-I failed to solve the problem of specificity satisfactorily in our hands (9).…”

Section: Discussionmentioning

confidence: 73%

“…A variety of tests are generally available for the laboratory diagnosis of hepatitis B antigen (HB-Ag), such as the agar gel diffusion, counterimmunoelectrophoresis (CIEP), complement fixation test (CFT), immunoelectromicroscopy, reversed passive hemagglutination (RPH), and direct radioimmunoassays (RIA) (3)(4)(5)(6)(7).…”

mentioning

confidence: 99%

“…We have excluded the agar gel diffusion because of its lack of sensitivity (4). The sensitivity of the CFT has been established previously (4,7). It has not gained widespread use because of its technical complications, although it is more sensitive than the CIEP.…”

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confidence: 99%

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Comparison of the reversed passive hemagglutination with radioimmunoassay methods for hepatitis B antigen

Leers¹,

Kouroupis²

1975

J Clin Microbiol

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Radioimmunoassay for the detection of hepatitis B antigen has been accepted in many diagnostic laboratories now. The question of nonspecific positives has always been a matter of controversy. Two improved radioimmunoassay tests, namely Ausria II-125 by Abbott Laboratories and a radioimmunoassay method by Connaught Laboratories Limited (Hebria), were compared with the original Ausria 125I. Included in the comparison was the reversed passive hemagglutination test (Auscell, Abbott). Five hundred sera of clinical patients were tested. Fifty-five or 11% were found to have hepatitis B antigen. Three tests, Ausria 125I, Ausria II-125, and Hebria showed the same number of positive sera, whereas Auscell missed one. However, Ausria 125I indicated two additional false positives. Dilution experiments, however, indicated that Ausria II-125 and Hebria were the most sensitive tests, with the reversed passive hemaglutination showing the least sensitivity. Therefore, the new Ausria II-125 and the Hebria radioimmunoassay tests are preferable in view of their sensitivity and specificity.

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Section: Discussionmentioning

confidence: 73%

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confidence: 99%

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Comparison of the reversed passive hemagglutination with radioimmunoassay methods for hepatitis B antigen

Leers¹,

Kouroupis²

1975

J Clin Microbiol

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“…Der Ausria 11 125-Test wurde in der üblichen Prozedur mit Inkubationszeiten von zwei Stunden und einer Stunde im Wasserbad bei 45 oe und Zählung der Impulse für eine Minute ausgeführt. Zur Verfügung standen Kits der Lot-Nummer 71.959.BX 1. 4.…”

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Der Nachweis von HBs-Antigen mit dem Hepanostika-Test, einem Enzymimmunoassay

Lange¹,

Köhler²,

Apodaca³

et al. 1977

Dtsch med Wochenschr

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Sensitivity, specificity and practicability of the Hepanostika test, a newly developed enzyme immunoassay by organon Teknika for the demonstration of HBs-antigen, was compared with results of the Aursria II 125 test. Smallest demonstrable concentration of the antigen in the Hepanostika test eas 3.125 ng/ml with antigens of subgroup ad and 2.08 ng/ml in those of subgroup ay, while it was 1.04 ng/ml for both subgroups with the Austria test. Among 2930 blood donors 85 antigen carriers were discovered by both tests, while with the Hepanostison test, a passive haemagglutination test from Oranon, only nine were discovered. In serial blood samples of patients with various disease there was only a slight difference between the two tests. The Hepanostika test had 1.6--3.88% nonspecific reactions, compared with 0.5-0.8% with the Ausria II 125 test. Both tests thus gave much more specific results than passive haemagglutination. The Hepanostika test is thus a satisfactory alternative to the Ausria test. Because of its high sensitivity it can be recommended for the diagnosis of HBs antigen among blood donors and in clinical laboratories.

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“…CEP, although a quick test, is far less sensitive than RIA. Various other tests have been described, such as Ouchterlony double diffusion, red cell agglutination, reverse passive hemagglutination, latex agglutination, complement fixation, and immunoelectron microscopy (2,4,5,10,11), to name a few. Recently, a rapid hemagglutination test has been described (Wellcome Laboratories, England) which uses turkey erythrocytes coated with horse antibody to hepatitis B antigen purified by affinity column chromatography.…”

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Evaluation of turkey erythrocyte hemagglutination assay for the detection of hepatitis B antigen

Ramírez¹,

Tiku²,

Ogra³

1975

J Clin Microbiol

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A recently described hemagglutination test for hepatitis B antigen (HB.Ag) using turkey erythrocytes coated with horse antibody to HB.Ag purified by affinity column chromatography was evaluated on a comparative basis with 100 HB,Ag-positive and-negative serum samples. The turkey erythrocyte hemagglutination test (TEHA) was found to be less sensitive than radioimmunoassay (RIA) but gave far better results than counterimmunoelectrophoresis. Quantitative titration of HB.Ag in serial dilutions of the samples appeared to be more reliably performed by TEHA than by RIA. TEHA is a simple and sensitive technique for the detection of HB.Ag and may offer several practical advantages over RIA. Considerable evidence has accumulated to suggest a strong association between hepatitis B antigen (HB.Ag) and serum (type B, MS2,

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Radioimmunoassay, complement fixation and counter-immunoelectrophoresis in the laboratory diagnosis of hepatitis B

Cited by 6 publications

References 10 publications

Comparison of the reversed passive hemagglutination with radioimmunoassay methods for hepatitis B antigen

Comparison of the reversed passive hemagglutination with radioimmunoassay methods for hepatitis B antigen

Der Nachweis von HBs-Antigen mit dem Hepanostika-Test, einem Enzymimmunoassay

Evaluation of turkey erythrocyte hemagglutination assay for the detection of hepatitis B antigen

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