Der Nachweis von HBs-Antigen mit dem Hepanostika-Test, einem Enzymimmunoassay

Abstract

Sensitivity, specificity and practicability of the Hepanostika test, a newly developed enzyme immunoassay by organon Teknika for the demonstration of HBs-antigen, was compared with results of the Aursria II 125 test. Smallest demonstrable concentration of the antigen in the Hepanostika test eas 3.125 ng/ml with antigens of subgroup ad and 2.08 ng/ml in those of subgroup ay, while it was 1.04 ng/ml for both subgroups with the Austria test. Among 2930 blood donors 85 antigen carriers were discovered by both tests, while with the Hepanostison test, a passive haemagglutination test from Oranon, only nine were discovered. In serial blood samples of patients with various disease there was only a slight difference between the two tests. The Hepanostika test had 1.6--3.88% nonspecific reactions, compared with 0.5-0.8% with the Ausria II 125 test. Both tests thus gave much more specific results than passive haemagglutination. The Hepanostika test is thus a satisfactory alternative to the Ausria test. Because of its high sensitivity it can be recommended for the diagnosis of HBs antigen among blood donors and in clinical laboratories.

Enzyme immunoassays with special reference to ELISA techniques.

“…This was commercialised as Hepanostika (Organon Teknika), which several independent groups have found to be at least as sensitive as currently available RIA kits (Vandervelde et al, 1977;Lange et al, 1977;Ukkonen et al, 1977). Specificity and confirmatory controls are essential, and the simple blocking procedure has provided these in a convenient form.…”