Radiographic assessment of photodynamic therapy as an adjunctive treatment on induced periodontitis in immunosuppressed rats

Abstract

ObjectiveThe aim of this study was to assess radiographically the effect of photodynamic therapy (PDT) as an adjunctive treatment to scaling and root planing (SRP) on induced periodontitis in dexamethasone-induced immunosuppressed rats.Material and MethodsThe animals were divided into 2 groups: ND group (n=60): saline treatment; D group (n=60): dexamethasone treatment. In both ND and D groups, periodontal disease was induced by the placement of a ligature in the left first mandibular molar. After 7 days, ligature was removed and all animals received SRP, being divided according to the following treatments: SRP: saline and PDT: phenothiazinium dye (TBO) plus laser irradiation. Ten animals per treatment were killed at 7, 15 and 30 days. The distance between the cementoenamel junction and the height of the alveolar bone crest in the mesial surface of the mandibular left first molars was determined in millimeters in each radiograph. The radiographic values were analyzed statistically by ANOVA and Tukey's test at a p value <0.05.ResultsIntragroup radiographic assessment (ND and D groups) showed that there was statistically significant less bone loss in the animals treated with PDT in all experimental periods compared to those submitted to SRP. Intergroup radiographic analysis (ND and D groups) demonstrated that there was greater bone loss in the ND group treated with SRP compared to the D group treated with PDT at 7 and 30 days.ConclusionPDT was an effective adjunctive treatment to SRP on induced periodontitis in dexamethasone-induced immunosuppressed rats.

Melatonin prevents radiation‐induced oxidative stress and periodontal tissue breakdown in irradiated rats with experimental periodontitis

“…The right and left lower‐first molars of the rats in Ped, Ped‐Rt, Ped‐Mel and Ped‐Rt‐Mel groups were ligatured under anesthesia [10 mg/kg of xylazine (Rompun; Bayer, İstanbul, Turkey) and 40 mg/kg of ketamine (Ketalar; Pfizer, Istanbul, Turkey)] with 3‐0 sterile silk sutures, and the ligatures were kept in place for 2 wk, according to previous studies .…”

Periodontitis and Photodynamic Treatment: Review

“…Fernandes ve ark., immünosüpresif ilaç verdikleri deney hayvanlarında radyografik olarak belirlenen periodontal yıkımın "scaling and root planing (SRP)"e ilave olarak FDT ile tedavi edilen grupta yalnızca SRP yapılan gruba göre daha az olduğunu bildirmişlerdir. 41 Fernandes ve ark. bir diğer benzer çalışmalarında FDT'nin periodontitte destekleyici tedavi olarak kullanılabileceğini rapor etmişlerdir.…”

Influência de raízes tratadas quimicamente e com laser sobre a proliferação de fibroblastos gengivais humanos e granulação óssea

“…Sobre as células de granulação óssea os resultados não foram melhores estatisticamente para este grupo, mas também se pode afirmar que não houve morte celular, o que sugere que também não haja citotoxidade deste corante para as células ósseas. Fernandes et al (2010) observaram radiograficamente que a perda óssea em bolsas induzidas em ratos imunossuprimidos tratados com PDT, foi significativamente menor do que nos tratados somente com RAR.…”

“…A Terapia Fotodinâmica é uma modalidade de tratamento que se baseia na ativação, por uma fonte de luz, de agentes fotossensibilizadores exógenos ou corantes (FERNANDES et al, 2010;SALMERON et al, 2013). Consiste no uso de um corante (azul de toluidina) associado a um laser em baixa intensidade com comprimento de onda correspondente ao corante, como por exemplo, o laser de InGaAsP que emite espectro de luz vermelha visível com comprimentos de onda que variam de 600nm a 680nm e potência entre 10 a 50mW (WALSH, 1997;GENOVESE, 2000).…”