Radiographic analysis to test maize seeds for the presence of <i>Sitophilus zeamais</i> (Coleoptera: Curculionidae)

Silva, Fabiano França da; Carvalho, María Laene Moreira de; Carvalho, G. A.; Andrad, D. B. de; Souza, Vander Fillipe de; Marques, Eduardo Resende Girardi

doi:10.15258/sst.2019.47.3.02

Cited by 6 publications

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“…During this period, foreign scientists also advanced in research on seed radiography. Different works in the field of comprehensive analysis of the quality of grain of cereals and seeds of industrial crops were developed [25][26][27][28]. The method of X-ray diffraction of seeds also began to be used to solve various problems of biology and seed science [29][30].…”

Section: Resultsmentioning

confidence: 99%

History and prospects of the application of X-ray in seed production and seed studies

Мусаев

Потрахов

Beletskiy³

2020

E3S Web Conf.

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A retrospective analysis of the history of the application of the method of radiography in the course of studies of the structure of seeds for the purposes of seed production and seed studies was carried out. It is shown that the beginning of X-ray studies of seeds is directly related to the development in the late 70s of the last century in the Special Design Bureau for X-ray Instruments, Leningrad Association of Electronic Instrumentation “Svetlana” of a specialized X-ray apparatus based on the X-ray emitter REIS with a voltage of 25 kV. The methodological part of the research was provided by the leading specialized enterprises of our country, now they are FSBSI API, FSBI Research Institute for Storage Problems of the Federal Reserve FSBSI FSVC. In the early 2000s, ETU “LETI”, represented by a small enterprise of the Technopark LETI, CJSC “ELTECH-Med”, actively joined the research. LETI is associated with the transition to digital receivers of X-ray images, which made it possible to fundamentally increase the efficiency of research by employees of CJSC “ELTECH-Med” together with specialists from Agrophysical Research Institute, FSBI Research Institute for Storage Problems of the Federal Reserve, FSBSI FSVC developed a number of technical means for obtaining digital 2 and 3 dimensional X-ray images of seeds.

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Section: Resultsmentioning

confidence: 99%

History and prospects of the application of X-ray in seed production and seed studies

Мусаев

Потрахов

Beletskiy³

2020

E3S Web Conf.

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“…In the traditional "infested seed examination" methodology, the analyst inspects 200 seeds individually, using sharp tools and observing signs of insect infestation on the external surface and damages to the internal structures of the seed. It is a time-consuming methodology, which leads to the analyst's visual fatigue, ergonomic problems, possible risks to the analyst's health due to the use of sharp blades, and compromises the quality of the analyses (Carvalho et al, 2019;França-Silva et al, 2019;Gomes-Junior, 2019).…”

Section: Core Ideasmentioning

confidence: 99%

Determination of Sitotroga cerealella infestation in wheat seeds by radiographic and multispectral images

et al. 2020

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Radiographic and multispectral image analysis have potential to be efficient, objective methods for assessing seed quality and internal insect infestation. The aim of this study was to verify the efficiency of radiographic and multispectral analysis in detecting signs and damage caused by Angoumois grain moth [Sitotroga cerealella (Olivier)] and its different developmental stages in wheat (Triticum aestivum L.) seeds. The experiment was conducted in a completely randomized design with six replications of 50 seeds. The samples were subjected to laboratory-induced infestation and after 5 and 10 d, radiographic and multispectral analysis were conducted. Afterwards, the seeds were immersed in water for 24 h and then sectioned with a cutting blade. The number of seeds with signs of eggs or oviposition, larvae, pupae, adult insects and insect galleries was quantified. The generalized linear models (GLM) methodology was used and the Tukey test (p < .05) was used to compare the means. In general, the radiographic (with or without contrast) and multispectral methods are viable tools to evaluate insect-infested and uninfested wheat seeds. Multispectral analysis was efficient only in identifying eggs on the seed surface and does not detect the presence of larvae and pupae inside the seeds.

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“…Зарубежные ученые за данный период также продвинулись в исследованиях по рентгенографии семян. Развитие нашли работы в области комплексного анализа качества зерна злаковых и семян технических культур [21][22][23][24]. Метод рентгенографии семян также стал применяться для решения различных задач биологии и семеноведения [25,26].…”

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History and Prospects for the Application of X-Ray Diffraction Analysis in Seed Breeding and Seed Study

Мусаев

Beletskiy

2021

Izv. vysš. učebn. zaved. Ross., Radioèlektron.

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Introduction. X-ray analysis has been applied for visualizing the internal structure of various objects for over 100 years. However, this method began to be used for assessing the quality of plant seeds only in the early 1980s. The main impediment was a lack of specialized instruments, particularly X-ray sources, that could provide informative images. Advancements in the field of microfocus radiography allowed significant results to be achieved, including the preparation of the National Standard GOST R 596032021 "Agricultural Seeds. Methods of digital radiography".Aim. An analytical review of Russian research studies in the field of X-ray diffraction analysis of plant seeds.Materials and methods. Key stages in the development of microfocus X-ray diffraction analysis of seeds and individual parts of plants for agricultural and other purposes are considered. The design of instruments, including digital ones, created for the implementation of the method are described.Results. In order to obtain informative X-ray diffraction images of plant seeds, which objects are generally characterized by small sizes and small density, the focal spot of the X-ray tube should not exceed several tens of microns under the voltage of not higher than several tens of kilovolts. As a system for visualizing a latent X-ray image, it is preferable to use image receivers based on a screen with a photostimulated phosphor or flat-panel solid-state X-ray detectors. These instruments have been successfully used to identify and describe the radiographic signs of a normal seed and nine main types of defects for 600 plant species.Conclusion. In comparison with the conventional contact radiography, microfocus radiography produces X-ray images of seeds with a projection magnification of the image up to several tens of times. Such images permit highly detailed visualization of the structure of seeds that differ slightly in density.

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Radiographic analysis to test maize seeds for the presence of Sitophilus zeamais (Coleoptera: Curculionidae)

Cited by 6 publications

References 0 publications

History and prospects of the application of X-ray in seed production and seed studies

History and prospects of the application of X-ray in seed production and seed studies

Determination of Sitotroga cerealella infestation in wheat seeds by radiographic and multispectral images

History and Prospects for the Application of X-Ray Diffraction Analysis in Seed Breeding and Seed Study

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