Radioelectric Asymmetric Conveyed Fields and Human Adipose-Derived Stem Cells Obtained with a Nonenzymatic Method and Device: A Novel Approach to Multipotency

Abstract

Human adipose-derived stem cells (hASCs) have been recently proposed as a suitable tool for regenerative therapies for their simple isolation procedure and high proliferative capability in culture. Although hASCs can be committed into different lineages in vitro, the differentiation is a low-yield and often incomplete process. We have recently developed a novel nonenzymatic method and device, named Lipogems, to obtain a fat tissue derivative highly enriched in pericytes/mesenchymal stem cells by mild mechanical forces from human lipoaspirates. When compared to enzymatically dissociated cells, Lipogems-derived hASCs exhibited enhanced transcription of vasculogenic genes in response to provasculogenic molecules, suggesting that these cells may be amenable for further optimization of their multipotency. Here we exposed Lipogems-derived hASCs to a radioelectric asymmetric conveyer (REAC), an innovative device asymmetrically conveying radioelectric fields, affording both enhanced differentiating profiles in mouse embryonic stem cells and efficient direct multilineage reprogramming in human skin fibroblasts. We show that specific REAC exposure remarkably enhanced the transcription of prodynorphin, GATA-4, Nkx-2.5, VEGF, HGF, vWF, neurogenin-1, and myoD, indicating the commitment toward cardiac, vascular, neuronal, and skeletal muscle lineages, as inferred by the overexpression of a program of targeted marker proteins. REAC exposure also finely tuned the expression of stemness-related genes, including NANOG, SOX-2, and OCT-4. Noteworthy, the REAC-induced responses were fashioned at a significantly higher extent in Lipogems-derived than in enzymatically dissociated hASCs. Therefore, REAC-mediated interplay between radioelectric asymmetrically conveyed fields and Lipogemsderived hASCs appears to involve the generation of an ideal "milieu" to optimize multipotency expression from human adult stem cells in view of potential improvement of future cell therapy efforts.

Cancer Stem Cells: Foe or Reprogrammable Cells for Efficient Cancer Therapy?

“…Similar results are obtained upon exposure to ACEF of ADhMSCs [75]. This effect is the result of a fine modulation (initial increase and subsequent transcriptional inhibition) of the expression of stemness related genes [75].…”

In vitro extracorporeal shock wave treatment enhances stemness and preserves multipotency of rat and human adipose-derived stem cells

“…Physical stimuli have been shown to have beneficial effects on isolation of hASCs [radio electric asymmetric conveyed fields [20]] and differentiation into osteoblasts [controlled frequency ultrasound [21]] or chondrocytes [combination of shear and dynamic compression [46]]. The advantageous effects of in vivo extracorporeal shock wave therapy in several fields of regenerative medicine have been known for more than a decade, whereas in vitro effects have not been studied extensively yet.…”

“…Interestingly, in this regard, physical stimuli such as controlled frequency ultrasound [20], shear stress and dynamic compression [21], but also extracorporeal shock waves [22], have been shown to have positive effects on stem cell isolation and specific differentiations, whereas only little is known concerning effects on stemness.…”

Life Rhythm as a Symphony of Oscillatory Patterns: Electromagnetic Energy and Sound Vibration Modulates gene Expression for Biological Signaling and Healing

“…Experiments from our group found similar results, using a 2.4 GHz electrode directly immersed in the cell culture medium. For these exposure conditions, we observed enhanced transcription of prodynorphin, GATA-4, Nkx-2.5, VEGF, HGF, vWF, neurogenin-1 and myoD, indicating commitment toward cardiac, vascular, neuronal and skeletal muscle lineages and alteration in expression of stemness-related genes, including Nanog, Sox-2, and Oct-4 in adipose-derived stem cells 183 . Recently, we have also observed direct reprogramming of human dermal skin fibroblasts into cardiac, neuronal and skeletal muscle lineages 184 .…”