Radiochemical Determination of the Metabolic Activity of Collagen in Mature Dentin

Borggreven, J.M.P.M.; Hoppenbrouwers, P.M.M.; Görissen, Ralf

doi:10.1177/00220345790580111401

Cited by 14 publications

(5 citation statements)

References 8 publications

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“…Deoxypyridinoline (dPyr), is a minor cross-link and found predominantly in bone and dentin (Robins 1990). Due to slow turn over of dentin, dPyr is considered to be bone specific (Borggreven 1979). Urinary excretion of hydroxyl proline is also one of the commonly used indices of bone resorption.…”

Section: Discussionmentioning

confidence: 99%

The beneficial effect of OST-6 (OsteoCare), a herbomineral formulation, in experimental osteoporosis

Mitra¹,

Venkataranganna²,

Udupa³

et al. 2001

Phytomedicine

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Section: Discussionmentioning

confidence: 99%

The beneficial effect of OST-6 (OsteoCare), a herbomineral formulation, in experimental osteoporosis

Mitra¹,

Venkataranganna²,

Udupa³

et al. 2001

Phytomedicine

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“…Once laid down, dentin collagen is thought not to turn over metabolically, either during growth or in the adult tooth [17][18][19]. Slow thickening of root dentin goes on throughout adulthood, by appositional growth on pulpal surfaces [ 16].…”

Section: Discussionmentioning

confidence: 99%

Collagen crosslinks in human dentin: Increasing content of hydroxypyridinium residues with age

Walters

Eyre

1983

Calcif Tissue Int

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The hydroxypyridinium crosslinks of collagen are believed to derive from reducible, divalent crosslinks. To study this concept further, both types of crosslink were quantified as a function of age in dentin, a tissue thought to have minimal collagen turnover. Human (5, 15, 28 and 56 years) and bovine (fetal and adult) root dentin was analyzed by a procedure that measures both hydroxypyridinium and reducible crosslinks on the amino acid analyzer. In human dentin, hydroxypyridinium crosslinks increased with age and became the predominant crosslinks as the two reducible residues, dehydrodihydroxylysinonorleucine and dehydrohydroxylysinonorleucine, diminished. Similarly in adult bovine dentin, hydroxypyridinium residues were sixfold more concentrated than in fetal bovine dentin. Borohydride treatment of tissue did not influence the measured content of hydroxypyridinium residues. The analyses also ruled out natural reduction as a stabilizing reaction for the divalent, reducible crosslinks. Though hydroxypyridinium residues became the major aldehyde-mediated crosslinks of adult dentin collagen, significant levels of reducible crosslinks remain throughout the tooth's adult life.

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“…The NCPs include phosphorylated proteins such as SIBLINGs (small integrin-binding ligand N-linked glycoproteins), SLRPs (small leucine-rich proteoglycans), amelogenin and proteolipids, and some nonphosphorylated proteins like osteocalcin, MGP (Matrix-Gla Protein), osteonectin, proteins from the blood serum (albumin and fetuin), SLRPs, growth factors, enzymes, polyamines, and calcium binding proteins . Of particular interest is that collagen in dentine has been shown to be metabolically stable during life, and its turnover is so low that it is assumed negligible; ,, for this reason, dentine is the tissue of choice in forensics when there is the need to estimate the age of remains using techniques such as radiocarbon dating and aspartic acid (Asp) racemization analysis. ,,− …”

Section: Introductionmentioning

confidence: 99%

Exploring Biological and Geological Age-related Changes through Variations in Intra- and Intertooth Proteomes of Ancient Dentine

Procopio

Buckley

2018

J. Proteome Res.

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Proteomic analyses are becoming more widely used in archeology not only due to the greater preservation of proteins in ancient specimens than DNA but also because they can offer different information, particularly relating to compositional preservation and potentially a means to estimate biological and geological age. However, it remains unclear to what extent different burial environments impact these aspects of proteome decay. Teeth have to date been much less studied than bone but are ideal to explore how proteins decay with time due to the negligible turnover that occurs in dentine relative to bone. We investigated the proteome variability and deamidation levels of different sections of molar teeth from archeological bovine mandibles as well as their mandibular bone. We obtained a greater yield of proteins from the crown of the teeth but did not find differences between the different molars analyzed within each mandible. We also obtained the best variety of protein from a well-preserved mandible that was not the youngest one in terms of chronological age, showing the influence of the preservation conditions on the final proteomic outcome. Intriguingly, we also noticed an increase in abundance levels of fetuin-A in biologically younger mandibles as reported previously, but the opposite trend in tooth dentine. Interestingly, we observed higher glutamine deamidation levels in teeth from the geologically oldest mandible despite it being the biologically youngest specimen, showing that the archeological age strongly impacts on the level of deamidations observed, much more so than biological aging. This indicates that the glutamine deamidation ratio of selected peptides may act as a good predictor of the relative geochronological age of archeological specimens.

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Radiochemical Determination of the Metabolic Activity of Collagen in Mature Dentin

Cited by 14 publications

References 8 publications

The beneficial effect of OST-6 (OsteoCare), a herbomineral formulation, in experimental osteoporosis

The beneficial effect of OST-6 (OsteoCare), a herbomineral formulation, in experimental osteoporosis

Collagen crosslinks in human dentin: Increasing content of hydroxypyridinium residues with age

Exploring Biological and Geological Age-related Changes through Variations in Intra- and Intertooth Proteomes of Ancient Dentine

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