Radioautographic Studies of the Dna and Rna Synthesizing Capacity of the Human Germinal Epithelium in Vitro

In vitro autoradiographic studies of DNA synthesis in human testis with spermatogenic arrest

“…These authors point out that the variation in intratubular glycogen could be related to the high energy required in nucleic acid synthesis in the first phases of spermatogenesis. Previously Markewitz et al (1967) had shown with in vitro autoradiographic studies after labelling with 3H-thymidine that in human spermatogenic arrest there was a marked reduction in the number of tubules with labelled cells and of labelled cells per individual tubule. In these conditions there is, therefore, a reduction in basic germ cells, namely spermatogonia and primary spermatocytes.…”

“…In this paper, we report on a study of DNA synthesis in the germ cells which was planned to confirm the differences found by Markewitz et al (1967) between normal spermatogenesis and spermatogenetic arrest, and furthermore to investigate whether there are differences in the amount of DNA synthesis in the testes with spermatidic and spermatocytic arrest.…”