A Comparative study of Germ Cell Kinetics in the Testes of Children with Unilateral Cryptorchidism: A Preliminary Report

Markewitz, Moshe; Lattimer, John K.; Veenema, Ralph J.

doi:10.1016/s0015-0282(16)37867-0

Cited by 14 publications

(1 citation statement)

References 8 publications

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“…In this study, the immunohistochemical localization of PCNA was found in the spermatogonia in both cryptorchid and contrascrotal testes, and the number of PCNA-positive germ cells was decreased in cryptorchid testes. A decrease in spermatogonium proliferation activity was observed in spontaneous unilateral cryptorchid boys [ 34 ], dogs [ 16 ], and boars [ 15 ], as well as in an experimentally induced cryptorchid rabbit model [ 8 ]. Although the proliferation activity decreased, the low proliferative activity suggested that the number of spermatogonia increased via proliferation in the cryptorchid testes.…”

Section: Discussionmentioning

confidence: 99%

Effect of Cryptorchidism on the Histomorphometry, Proliferation, Apoptosis, and Autophagy in Boar Testes

Fan

Liu

Yue

et al. 2021

Animals

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Spontaneous unilateral cryptorchid boars have one testis in the abdomen or inguinal canal, causing its temperature to be at or near the body temperature, which impairs spermatogenesis, although the histomorphometry and molecular mechanisms underlying this process remain unclear. The aim of the present study was to determine the histomorphometry, proliferation, apoptosis, and autophagy alterations in spermatogonia and Sertoli cells in unilateral cryptorchid, scrotal (contrascrotal), and preweaning piglet (preweaning) testes. Histomorphometrical analysis of cryptorchid testes showed that the seminiferous tubules contained only Sertoli cells and a few spermatogonia, but did not contain post-meiotic germ cells. The number of spermatogonia markedly decreased, and the number of Sertoli cells did not change remarkably in cryptorchid testes. TUNEL assay results showed that apoptosis signals were predominantly observed in spermatogonia. In cryptorchid and contrascrotal testes, proliferating cell nuclear antigen (PCNA) and LC3 were located in spermatogonia. The number of PCNA-positive, TUNEL-positive, and LC3-positive germ cells was low, and the protein and mRNA levels of PCNA and LC3 were significantly decreased in cryptorchid testes. Taken together, the number of Sertoli cells did not change remarkably, whereas the number of germ cells decreased in the cryptorchid testes, compared with that in the contrascrotal testes. Insufficient proliferation, excessive apoptosis, and autophagy were involved in the regulation of the decrease in spermatogonia in cryptorchid boar testes.

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