Proliferation and apoptosis of spermatogonia in postpuberal boar (Sus domesticus) testes with spontaneous unilateral and bilateral abdominal cryptorchidism

Bernal-Mañas, C M; Morales, Eva; Pastor, Luis Miguel; Pinart, E.; Bonet, Sergi; Rosa, Pedro de la; Briz, M.; Zuasti, Adelina; Ferrer, Carmen María Salvador; Canteras, Manuel

doi:10.1016/j.acthis.2005.07.002

Cited by 20 publications

(17 citation statements)

References 34 publications

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“…TBT intake of male adult rats in two-generation toxicity study induced mild testicular histological changes which were vacuolization of seminiferous epithelium, spermatid retention in the epithelium, delayed spermiation, and germ cell degeneration (Omura et al, 2001). As indicated, homeostasis of the seminiferous epithelium depends on cell death and on the proliferative activity of the epithelium, and any imbalance of the two processes may result in histological changes (Bernal-Manas et al, 2005). Nuclear protein proliferating cell nuclear antigen (PCNA) is essential for multiple cell-cycle pathways, such as the initiation of DNA duplication (Schlatt and Weinbauer, 1994).…”

Section: Discussionmentioning

confidence: 98%

Reduction of spermatogenesis in mice after tributyltin administration

et al. 2008

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Section: Discussionmentioning

confidence: 98%

Reduction of spermatogenesis in mice after tributyltin administration

et al. 2008

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“…In adult rat testis, heat shock (43°C for 15 min) caused germinal epithelium damage, abnormal Sertoli cell and germ cell death [11], [131], [132] that mainly occurred to meiotic spermatocytes and early spermatids [11]. The intrinsic mitochondria-dependent pathway proved critical for heat-induced germ cell apoptosis, in which Bcl-2 family members played important functions [77], [81], [133], [134], [135]. Bax induced the cyt C release from the mitochondria to initiate the activation of Caspase9, which eventually activated downstream effector caspases (caspase3, 6 and 7) leading to cell death [81], [136].…”

Section: Discussionmentioning

confidence: 99%

The Apoptotic Function Analysis of p53, Apaf1, Caspase3 and Caspase7 during the Spermatogenesis of the Chinese Fire-Bellied Newt Cynops orientalis

Wang

et al. 2012

PLoS ONE

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BackgroundSpontaneous and stress-induced germ cell apoptosis during spermatogenesis of multicellular organisms have been investigated broadly in mammals. Spermatogenetic process in urodele amphibians was essentially like that in mammals in spite of morphological differences; however, the mechanism of germ cell apoptosis in urodele amphibians remains unknown. The Chinese fire-belly newt, Cynops orientalis, was an excellent organism for studying germ cell apoptosis due to its sensitiveness to temperature, strong endurance of starvation, and sensitive skin to heavy metal exposure.Methodology/Principal FindingsTUNEL result showed that spontaneous germ cell apoptosis took place in normal newt, and severe stress-induced apoptosis occurred to spermatids and sperm in response to heat shock (40°C 2 h), cold exposure(4°C 12 h), cadmium exposure(Cd 36 h), and starvation stress. Quantitative reverse transcription polymerase chain reactions (qRT-PCR) showed that gene expression of Caspase3 or Caspase7 was obviously elevated after stress treatment. Apaf1 was not altered at its gene expression level, and p53 was significantly decreased after various stress treatment. Caspase assay demonstrated that Caspase-3, -8,-9 enzyme activities in newt testis were significantly elevated after heat shock (40°C 2 h), cold exposure(4°C 12 h), and cadmium exposure(Cd 36 h), while Caspase3 and Caspase8 activities were increased with Caspase9 significantly decreased after starvation treatment.Conclusions/SignificanceSevere germ cell apoptosis triggered by heat shock, cold exposure, and cadmium exposure was Caspase3 dependent, which probably involved both extrinsic and intrinsic pathways. Apaf1 may be involved in this process without elevating its gene expression. But starvation-induced germ cell apoptosis was likely mainly through extrinsic pathway. p53 was probably not responsible for stress-induced germ cell apoptosis in newt testis. The intriguing high occurrence of spermatid and sperm apoptosis probably resulted from the sperm morphology and unique reproduction policy of Chinese fire-belly newt, Cynops orientalis.

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“…; Bernal‐Mañas et al. ) and immunocytochemical techniques, which localize the main apoptotic proteins such as Fas/Fas‐L, Bax, Bak, Bcl‐xs and Bad (Beumer et al. ; Miura et al.…”

Section: Introductionmentioning

confidence: 99%

“…The histological techniques used to identify apoptotic cells in the seminiferous epithelium are in situ terminal deoxynucleotidyl transferase dNTP end labelling (TUN-EL) (Young and Nelson 2001b;Young et al 2001a;Bernal-Mañas et al 2005) and immunocytochemical techniques, which localize the main apoptotic proteins such as Fas/Fas-L, Bax, Bak, Bcl-xs and Bad (Beumer et al 2000;Miura et al 2002;Morales et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Lectin Histochemistry as a Tool to Identify Apoptotic Cells in the Seminiferous Epithelium of Syrian Hamster (Mesocricetus auratus) Subjected to Short Photoperiod

Seco-Rovira

Beltrán-Frutos

Ferrer

et al. 2013

Reprod Domestic Animals

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Lectins have been widely used to study the pattern of cellular glycoconjugates in numerous species. In the process of cellular apoptosis, it has been observed that changes occur in the membrane sugar sequences of these apoptotic cells. The aim of our work was to identify which lectins, out of an extensive battery of the same (PNA, SBA, HPA, LTA, Con-A, UEA-I, WGA, DBA, MAA, GNA, AAA, SNA), show affinity for germinal cells in apoptosis, at what stage of cell death they do so and in which germinal cell types they can be detected. For this, we studied testis sections during testicular regression in Syrian hamster (Mesocricetus auratus) subjected to short photoperiod. Several lectins showed an affinity for the glycoconjugate residues of germ cells in apoptosis: Gal β1,3-GalNAcα1, α-d-mannose, N-acetylgalactosamine and l-fucose. Furthermore, lectin specificity was observed for some specific germinal cells and in certain stages of apoptosis. It was also observed that one of these lectins (PNA) showed affinity for Sertoli cells undergoing apoptosis. Therefore, we conclude that the use of lectin histochemistry could be a very useful tool for studying apoptosis in the seminiferous epithelium because of the specificity shown towards germinal cells in pathological or experimentally induced epithelial depletion models.

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Proliferation and apoptosis of spermatogonia in postpuberal boar (Sus domesticus) testes with spontaneous unilateral and bilateral abdominal cryptorchidism

Cited by 20 publications

References 34 publications

Reduction of spermatogenesis in mice after tributyltin administration

Reduction of spermatogenesis in mice after tributyltin administration

The Apoptotic Function Analysis of p53, Apaf1, Caspase3 and Caspase7 during the Spermatogenesis of the Chinese Fire-Bellied Newt Cynops orientalis

Lectin Histochemistry as a Tool to Identify Apoptotic Cells in the Seminiferous Epithelium of Syrian Hamster (Mesocricetus auratus) Subjected to Short Photoperiod

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