Radioautographic Studies in Vitro of DNA and RNA Synthesis in Experimentally Produced Cryptorchidism in the Fischer Rat

Markewitz, Moshe; Fingerhut, B; Veenema, Ralph J.

doi:10.1016/s0015-0282(16)35950-7

Cited by 11 publications

(3 citation statements)

References 11 publications

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“…Flow cytometry was used, but is expensive and involves disruption of the testicular germ cells from tissue context [39]. Tritiatedthymidine autoradiography was also used, but is a complicated maneuver [23]. The bromodeoxyuridine (BrdUrd) method was also practiced but soon was criticized for its complexity and because it involves preadministration of carcinogenic agents that obviate its clinical application…”

Section: Discussionmentioning

confidence: 99%

Impact of Aging and Diabetes Mellitus on the Expression of the Proliferating Cell Nuclear Antigen in Rat Testicular Tissue

Salama¹,

Tsuji²,

Tamura³

et al. 1998

Archives of Andrology

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Section: Discussionmentioning

confidence: 99%

Impact of Aging and Diabetes Mellitus on the Expression of the Proliferating Cell Nuclear Antigen in Rat Testicular Tissue

Salama¹,

Tsuji²,

Tamura³

et al. 1998

Archives of Andrology

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“…The study of cell kinetics has contributed to the understanding of spermatogenesis (Clermont & Harvey, 1965). Until quite recently, the tritiated thymidine (3H-thymidine) technique has been the only method available to evaluate spermatogenic DNA synthesis (Markewitz et al, 1966;Lambert & Eriksson, 1979;Parvinen et al, 1991). However, this procedure is time consuming and requires radioactive tracers which limit experimental protocols.…”

Section: Introductionmentioning

confidence: 99%

Quantitative analysis of spermatogenic DNA synthesis in the rat using a monoclonal anti-5-bromodeoxyuridine antibody

et al. 2009

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The utility of the 5-bromodeoxyuridine (BrdUrd) labelling technique for the quantitative analysis of spermatogenic deoxyribonucleic acid (DNA) synthesis was investigated in the rat. Rat testicles were labelled by a single intraperitoneal injection of 100 mg kg-' of BrdUrd. The testicles were removed 1 h after injection, fked in Bouin's fluid and embedded in paraffin. BrdUrdlabelled cells were detected by immunohistochemical staining using a monoclonal anti-BrdUrd antibody. The number of BrdUrd-labelled tubules per total number of tubules (percent L.T.), the number of BrdUrd-labelled cells per total number of tubules (tubular ratio) and the number of BrdUrd-labelled cells per number of Sertoli cells (Sertoli cell ratio in BrdUrd-labelled cells) were calculated as indices of spermatogenic DNA synthesis during each stage of the seminiferous epithelial wave. BrdUrd labelling was found exclusively in the nuclei of spermatogonia and in preleptotene spermatocytes in the seminiferous epithelium. The percent L.T. was generally greater than 50%, except in stages VI, VII and XIV, and the tubular as well as Sertoli cell ratios in BrdUrdlabelled cells was greater than 2.0 and 0.15, respectively, in stages I, 11-111, v, VIII, X, and XII.The tubular ratio and Sertoli cell ratio in BrdUrdlabelled cells along the seminiferous epithelial wave had two distinct peaks. The distribution of the tubular ratio using the BrdUrd-labelling technique correlated well with the distribution previously established by measuring tritiated thymidine uptake per tubule. Thus, the BrdUrd labelling technique, which is more efficient than the tritiated thymidine labelling technique, can be used to quantitatively evaluate spermatogenic DNA synthesis.

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“…Changes in the concentration of various nucleic acid fractions in the experimentally-induced cryptorchid rat testis have been reported (McEnery & Nelson, 1953). Recently, radio-autographic studies of DNA and RNA synthesis in experimentally-produced cryptorchid testes have been reported (Markewitz, Fingerhut & Veenema, 1966). DNA and RNA synthesis was detected in the germinal epithelium in the presence of * Present address: Department of Pharmacology, Stanford University School of Medicine, Palo Alto, California. 325 326 Mannfred A. Hollinger and Joseph R. Davis degeneration of the seminiferous tubules and cessation of spermatogenesis.…”

Section: Introductionmentioning

confidence: 99%

Nucleic Acid Labelling of Experimentally-Induced Cryptorchid Rat Testis

Hollinger¹,

Davis²

1969

Reproduction

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The in vitro incorporation of [2-14C]thymidine and [2-14C]-uridine into DNA and RNA respectively, has been studied in slices of immature, mature, cryptorchid, and contralateral-scrotal testes of the rat. The results demonstrate that DNA and RNA labelling are both enhanced in the cryptorchid testis following 30 days in the abdominal cavity. Of the four tissues studied, the immature testis possessed the highest in vitro rate of DNA and RNA labelling. In addition, uptake of [2-14C]uridine into RNA of the contralateral-scrotal testis of cryptorchid rats is elevated.

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Radioautographic Studies in Vitro of DNA and RNA Synthesis in Experimentally Produced Cryptorchidism in the Fischer Rat

Cited by 11 publications

References 11 publications

Impact of Aging and Diabetes Mellitus on the Expression of the Proliferating Cell Nuclear Antigen in Rat Testicular Tissue

Impact of Aging and Diabetes Mellitus on the Expression of the Proliferating Cell Nuclear Antigen in Rat Testicular Tissue

Quantitative analysis of spermatogenic DNA synthesis in the rat using a monoclonal anti-5-bromodeoxyuridine antibody

Nucleic Acid Labelling of Experimentally-Induced Cryptorchid Rat Testis

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